Optimisation of somatic embryogenesis in fourteen cultivars of sweet potato [Ipomoea batatas (L.) Lam.]

Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

“…Frequencies ranged from no response at all to 17% when using lateral buds as explants. Many other observations of genotype dependence for in vitro regeneration in sweetpotato have been reported (Al-Mazrooei et al 1997;Thinh et al 1997).…”

Induction of somatic embryogenesis in recalcitrant sweetpotato (Ipomoea batatas L.) cultivars

“…However other workers report that 2,4,5-T is better than 2,4-D in inducing embryogenic callus (Al-Mazrooei et al, 1997;Triqui et al, 2007). Successful regeneration of transgenic plants from five Japanese cultivars has also been reported when 4-FA was used while there was no success with 2,4-D (Anwar et al, 2010).…”

“…In general, and particularly in important African sweetpotato cultivars, an efficient regeneration system has been very difficult to establish (Luo et al, 2006). Other reports show that there is a correlation between the quality and quantity of embryogenic callus and subsequent plant regeneration in sweetpotato (Al-Mazrooei et al, 1997). Therefore it is important to first establish a system for somatic embryogenesis for the recalcitrant cultivars to facilitate regeneration and enable application of genetic engineering to improve important traits (Anwar et al, 2010;Moran et al, 1998).…”

“…Up to five popular Ugandan cultivars were investigated since previous reports consistently show that cultivar has a high influence on somatic embryogenesis (Al-Mazrooei et al, 1997). Somatic embryogenesis was selected as a method of regeneration in this study because it results in high efficiency of selection during transformation since each whole plant originates from a single transformed cell (Sihachakr et al, 1997).…”

“…Efforts to extend this into a wide range of genotypes have shown that most of them are recalcitrant or respond at low frequencies (Al-Mazrooei et al, 1997;Anwar et al, 2010;Luo et al, 2006). Moreover regeneration protocols reported to work in some laboratories have proved difficult to reproduce in other laboratories (Moran et al, 1998).…”