Genetic Engineering

Kreuze, Jan; Valkonen, Jari P. T.; Ghislain, Marc

doi:10.1007/978-1-4020-9475-0_5

Cited by 12 publications

(14 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Progress in breeding weevil resistant cultivars has been slow because the heritability of the trait (Kreuze et al, 2009) is extremely low. Consequently, varieties with immunity or a high level of resistance are not yet available (Collins et al, 1991) despite the progress in finding weevil resistant components in some varieties (Stevenson et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Field Efficacy of Neem Seed Oil and Diazinon in the Management of Sweetpotato Weevil, Cylas puncticollis (Boh.) in South-eastern Nigeria

Ehisianya

Ukeh²,

Isah³

et al. 2013

JPS

View full text Add to dashboard Cite

The efficacy of the pre-and post-planting application of neem seed oil (NSO) or/and diazinon and time of harvest for the control of sweetpotato weevil, Cylas puncticollis (Boh.) was evaluated during the 2009 and 2010 cropping seasons. Forty-eight plots of 3 x 4 m 2 each were demarcated and 240 sweetpotato (var. TIS2532.op.1.13) vine cuttings were dipped into 0, 30 and 50 mls NSO and 30 mls of diazinon (60 EC) mixed in 2 and 10 litre soapy water, respectively and kept for 30 mins before planting. Similar treatment was also basally applied post-planting at 1MAP. In 2009, results showed that treatments (NSO or diazinon or their combinations) did not significantly influence percentage plant stand at harvest in both years, but lower percentage plant stands were obtained at 6 MAP (47.72%) than at 5MAP (56.87%). Significantly higher mean total weights of 34.44 t/ha was obtained in 2009 and 8.99 t/ha in 2010 respectively. Similarly, yield ranged from as high as 53.69 t/ha at 5MAP in 2009 to as low as 5.51t/ha at 6MAP in 2010. Significantly lower attributes of C. puncticollis as a pest were obtained in 2009 than in 2010. Treatments significantly suppressed root damage from 68.59% (Pr0Pt0) to 44.38% (Pr3Pt3) at 5 to 6MAP. Time of application was significant as 50 mls of NSO applied pre-and post-planting gave highest (35.30%) control of the pest at harvest.

show abstract

Section: Introductionmentioning

confidence: 99%

Field Efficacy of Neem Seed Oil and Diazinon in the Management of Sweetpotato Weevil, Cylas puncticollis (Boh.) in South-eastern Nigeria

Ehisianya

Ukeh²,

Isah³

et al. 2013

JPS

View full text Add to dashboard Cite

show abstract

“…These results, along with the fact that the Cry7Aa protoxin is processed to the 65-kDa fragment with C. puncticollis gut fluid, suggest that the 70-kDa fragment is further processed in vivo to the 65-kDa fragment. These results are highly relevant to the Bt sweet potato currently under development, because the transgene expresses the toxic fragment starting at position 59 upstream of the proteolytic cleavage site yielding the 65-kDa fragment (18). Thus, knowledge about the processing of the Cry proteins (at the N and C termini) may assist in the design of constructs for plant expression.…”

Section: Discussionmentioning

confidence: 97%

“…The beetle-specific Cry3Bb, Cry3Ca, and Cry7Aa proteins have been described as being active for the control of C. puncticollis (5), although little is known about the interaction of these proteins in the midgut of this insect. Recently, the cry3Ca and cry7Aa genes have been used independently to transform several sweet potato varieties with the aim of controlling sweet potato weevils (11,18). In addition, a modified Cry3Bb1 protein is expressed in transgenic corn hybrids, which are commercially available (YieldGard), to control corn rootworm larvae.…”

mentioning

confidence: 99%

Shared Binding Sites for the Bacillus thuringiensis Proteins Cry3Bb, Cry3Ca, and Cry7Aa in the African Sweet Potato Pest Cylas puncticollis (Brentidae)

Hernández‐Martínez

Vera-Velasco

Martínez-Solís

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

bBacillus thuringiensis Cry3Bb, Cry3Ca, and Cry7Aa have been reported to be toxic against larvae of the genus Cylas, which are important pests of sweet potato worldwide and particularly in sub-Saharan Africa. However, relatively little is known about the processing and binding interactions of these coleopteran-specific Cry proteins. The aim of the present study was to determine whether Cry3Bb, Cry3Ca, and Cry7Aa proteins have shared binding sites in Cylas puncticollis to orient the pest resistance strategy by genetic transformation. Interestingly, processing of the 129-kDa Cry7Aa protoxin using commercial trypsin or chymotrypsin rendered two fragments of about 70 kDa and 65 kDa. N-terminal sequencing of the trypsin-activated Cry7Aa fragments revealed that processing occurs at Glu 47 for the 70-kDa form or Ile 88 for the 65-kDa form. Homologous binding assays showed specific binding of the two Cry3 proteins and the 65-kDa Cry7Aa fragment to brush border membrane vesicles (BBMV) from C. puncticollis larvae. The 70-kDa fragment did not bind to BBMV. Heterologous-competition assays showed that Cry3Bb, Cry3Ca, and Cry7Aa (65-kDa fragment) competed for the same binding sites. Hence, our results suggest that pest resistance mediated by the alteration of a shared Cry receptor binding site might render all three Cry toxins ineffective.

show abstract

“…Shoot tips are a useful explant for induction of embryogenic callus in many plant species, including Ipomoea batatas (Liu et al 2001;Kreuze et al 2009;Liu 2011), and cereals such as Oryza sativa (Nhut et al 2000), Pennisetum glaucum (Jha et al 2009), and Sorghum bicolor (Seetharama et al 2000). Shoot tips are preferred over cells and calluses for cryopreservation of genetic resources, because of their high genetic stability (Sticklen and Oraby 2005;Panis and Lambardi 2006;Wang et al 2014a).…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from shoot regrowth and de novo embryo-like structures from cryopreserved shoot tips of Lilium spp.

Yin

Guo

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Plant regeneration via both shoot regrowth and de novo embryo-like structures was obtained from cryopreserved shoot tips of Lilium spp. Shoot tips (1.0-3.0 mm in length) of Lilium Oriental hybrid 'Siberia' containing two-to four-leaf primordia were excised from 4-wk-old adventitious shoots and cryopreserved by droplet vitrification. When postcultured for 6 wk on recovery medium containing 0.1 mg/l α-naphthaleneacetic acid (NAA) in combination with different kinetin (KT) concentrations (0-0.4 mg/l), three types of regenerants were produced: (1) only embryo-like structures, (2) shoot regrowth with embryo-like structures, and (3) only shoot regrowth. The highest frequencies (≥75.0%) of total embryo-like structures were obtained from cryopreserved shoot tips post-cultured on recovery medium containing 0.1 mg/l NAA and 0.1-0.2 mg/l KT. Embryo-like structures proliferated within 4 wk of culture on medium containing 1.0 mg/l NAA and 0.05 mg/l thidiazuron (TDZ). Somatic embryo-like structures matured and converted into whole plantlets after 8 wk of culture on Murashige and Skoog (MS) medium without any plant growth regulators. The highest frequencies (≥25.0%) of total shoot regrowth were produced from cryopreserved shoot tips post-cultured on recovery medium containing 0.1 mg/l NAA and 0.05-0.4 mg/l KT. With the protocols reported here, frequencies of total embryo-like structures and shoot regrowth ranged from 45.0 to 90.0% and 25.0 to 52.5%, respectively, from six lily varieties belonging to five Lilium species or hybrids, representing diverse Lilium germplasm. The results reported here have potential applications to long-term conservation of genetic resources and genetic transformation in Lilium spp.

show abstract

Genetic Engineering

Cited by 12 publications

References 69 publications

Field Efficacy of Neem Seed Oil and Diazinon in the Management of Sweetpotato Weevil, Cylas puncticollis (Boh.) in South-eastern Nigeria

Field Efficacy of Neem Seed Oil and Diazinon in the Management of Sweetpotato Weevil, Cylas puncticollis (Boh.) in South-eastern Nigeria

Shared Binding Sites for the Bacillus thuringiensis Proteins Cry3Bb, Cry3Ca, and Cry7Aa in the African Sweet Potato Pest Cylas puncticollis (Brentidae)

Plant regeneration from shoot regrowth and de novo embryo-like structures from cryopreserved shoot tips of Lilium spp.

Contact Info

Product

Resources

About