Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

Santa-María, Mónica C.; Pecota, Kenneth V.; Yencho, G. Craig; Allen, George C.; Sosinski, Bryon

doi:10.1007/s11240-009-9504-3

Cited by 22 publications

(13 citation statements)

References 21 publications

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“…More than 104 million tons are produced globally, 95% of which are grown in developing countries [1]. It is also an alternative source of bioenergy as a raw material for fuel production [2]. The orange-fleshed sweetpotato is rich in beta -carotene, which plays a crucial role in preventing vitamin A deficiency-related blindness and maternal mortality [3].…”

Section: Introductionmentioning

confidence: 99%

A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition

Huo

et al. 2016

BMC Genomics

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BackgroundSweetpotato, Ipomoea batatas (L.) Lam., is an important food crop widely grown in the world. However, little is known about the genome of this species because it is a highly heterozygous hexaploid. Gaining a more in-depth knowledge of sweetpotato genome is therefore necessary and imperative. In this study, the first bacterial artificial chromosome (BAC) library of sweetpotato was constructed. Clones from the BAC library were end-sequenced and analyzed to provide genome-wide information about this species.ResultsThe BAC library contained 240,384 clones with an average insert size of 101 kb and had a 7.93–10.82 × coverage of the genome, and the probability of isolating any single-copy DNA sequence from the library was more than 99%. Both ends of 8310 BAC clones randomly selected from the library were sequenced to generate 11,542 high-quality BAC-end sequences (BESs), with an accumulative length of 7,595,261 bp and an average length of 658 bp. Analysis of the BESs revealed that 12.17% of the sweetpotato genome were known repetitive DNA, including 7.37% long terminal repeat (LTR) retrotransposons, 1.15% Non-LTR retrotransposons and 1.42% Class II DNA transposons etc., 18.31% of the genome were identified as sweetpotato-unique repetitive DNA and 10.00% of the genome were predicted to be coding regions. In total, 3,846 simple sequences repeats (SSRs) were identified, with a density of one SSR per 1.93 kb, from which 288 SSRs primers were designed and tested for length polymorphism using 20 sweetpotato accessions, 173 (60.07%) of them produced polymorphic bands. Sweetpotato BESs had significant hits to the genome sequences of I. trifida and more matches to the whole-genome sequences of Solanum lycopersicum than those of Vitis vinifera, Theobroma cacao and Arabidopsis thaliana.ConclusionsThe first BAC library for sweetpotato has been successfully constructed. The high quality BESs provide first insights into sweetpotato genome composition, and have significant hits to the genome sequences of I. trifida and more matches to the whole-genome sequences of Solanum lycopersicum. These resources as a robust platform will be used in high-resolution mapping, gene cloning, assembly of genome sequences, comparative genomics and evolution for sweetpotato.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3302-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition

Huo

et al. 2016

BMC Genomics

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“…A higher number of shoots developed from protuberances on a medium containing 5.0 μM TDZ compared to a medium containing 10.0 μM TDZ (Table 1). Gosukonda et al (1995) and Santa-Maria et al (2009) also reported adventitious shoot induction from leaf explants of sweetpotato on a stage II medium, where the explants were incubated for a few days on a medium containing auxins, and were subsequently transferred to a medium supplemented with cytokinins, which resulted in the development of 1-2 shoots per explant with a significant genotype and media interaction. In this study, we obtained relatively few-times more shoots per explant compared to earlier reports Santa-Maria et al, 2009).…”

Section: Production Of Protuberances From Leaf Explantsmentioning

confidence: 96%

“…However, the lack of an efficient regeneration system is the major limiting factor which prevents the development of gene transfer technologies for commercially important cultivars of sweetpotato. There have been a number of proposals for the in vitro propagation of sweetpotato, including via adventitious shoot induction Dessai et al, 1995;Sihachakr et al, 1997;Santa-Maria et al, 2009) and somatic embryogenesis (Liu and Cantliffe, 1984;Chée et al, 1990;Desamero et al, 1994;Bieniek et al, 1995;Zheng et al, 1996;Dhir et al, 1998;He et al, 2009). Genetic transformation of sweetpotato, using various explants, has been reported, but with low efficiency (Newell et al, 1995;Gama et al, 1996;Morán et al, 1998;Okada et al, 2001;Berberich et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Kumar¹,

Ku²,

Agrawal³

et al. 2013

bta

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In this report, a novel, reproducible and efficient protocol for protuberance-mediated adventitious shoot regeneration has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron (TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants. It appeared that the frequency of protuberance development depended on the concentration of TDZ. However, this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully established in a greenhouse.

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“…For one plant species there exist several genotypes that differ in embryogenesis capacity. It is due to considerable variability of different genotypes in response to hormones . For instance, in the majority of cases frequency of flax embryo development reaches at average as few as 20%, the rest of it is root formation .…”

Section: Introductionmentioning

confidence: 99%

Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy

et al. 2019

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In the present study a new luminescent dye 3‐N‐(2‐pyrrolidinylacetamido)benzanthrone (AZR) was synthesized. Spectroscopic measurements of the novel benzanthrone 3‐aminoderivative were performed in seven organic solvents showing strong fluorescence. The capability of the prepared dye for visualization has been tested on flax, red clover and alfalfa to determinate the embryo in plant callus tissue cultures. Callus cells were stained with AZR and further analysed utilizing confocal laser scanning fluorescence microscopy. Performed experiments show high visualization effectiveness of newly synthesized fluorescent dye AZR that is efficient in fast and relatively inexpensive diagnostics of callus embryos that are problematic due to in vitro culture specificity.

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Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

Abstract: Sweetpotato (Ipomoea batatas L.

Cited by 22 publications

References 21 publications

A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition

A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy

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