Induction of somatic embryogenesis in recalcitrant sweetpotato (Ipomoea batatas L.) cultivars

Sefasi, A.; Kreuze, Jan; Ghislain, Marc; Manrique, S.; Kiggundu, Andrew; Ssemakula, G.; Mukasa, S.B.

doi:10.5897/ajb12.1615

Cited by 5 publications

(1 citation statement)

References 21 publications

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“…(Li et al, 2014), Gossypium spp. (Ghaemi, Majid, Fallahian, & Bezdi, 2011), Ipomea batatas L. (Sefasi et al, 2012) dan Manihot esculenta Crantz (Ngugi et al, 2015;Mongomake, Doungous, Khatabi, & Fondong, 2015). Perbedaan respons embriogenesis somatik di antara genotipe kakao juga telah dilaporkan sebelumnya oleh Avivi et al (2010); Ajijah et al (2014); dan Ajijah et al (2016).…”

Section: Pengaruh Interaksi Genotipe Jenis Eksplan Dan Taraf Kinetiunclassified

Pengaruh Sitokinin, Jenis Eksplan, dan Genotipe terhadap Embriogenesis Somatik Kakao

Ajijah¹,

Hartati²

2016

J. Tanaman Industri dan Penyegar

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Information on the effect of cytokinins on cacao (Theobroma cacao L.) primary somatic embryogenesis and its interaction with explant types and genotypes is not yet known. This study aimed to evaluate the effect of cytokinins and its interaction with explant types and genotypes on cacao somatic embryogenesis. The study was conducted at tissue culture laboratory of IAARD, Bogor from April until December 2012 and October 2014 until February 2016. Three types of cytokinins i.e. kinetin (0.58, 1.16, and 2.32 μM), thidiazuron (0.01, 0.02, and 0.04 μM) and benzylaminopurine (0.55, 1.11, and 2.22 μM) in combination with 9 μM 2,4-D were tested for their effectiveness in inducing somatic embryogenesis from petals and staminoid explants of Cimanggu 1 genotype. Furthermore, three levels of kinetin (0.58, 1.16, and 2.32 μM) also in combination with 9 μM 2,4-D were evaluated for their influences on the somatic embryogenesis from petals and staminoid explants of three cacao genotypes i.e. Sulawesi 02, ICCRI 04 and Cimanggu 3. The result demonstrated that 2.32 μM kinetin and staminoids explant were more effective to induce cacao somatic embryogenesis of Cimanggu 1 genotype (7%, 0.23 embryos/explant). Additionally, there were interaction effects between the level of kinetin with explant types and genotype on the percentage of explants forming embryo at 12 weeks after culture. The highest percentage of somatic embryo formation was shown by ICCRI 04 genotype with the use of petals explant and a kinetin level of 1.16 μM (31.85%), but not significantly different from the level of kinetin 2.23 μM (25.55%). The formation of primary somatic embryos of cacao is largely determined by the type and level of cytokinins, type of explant, and genotype.

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Section: Pengaruh Interaksi Genotipe Jenis Eksplan Dan Taraf Kinetiunclassified

Pengaruh Sitokinin, Jenis Eksplan, dan Genotipe terhadap Embriogenesis Somatik Kakao

Ajijah¹,

Hartati²

2016

J. Tanaman Industri dan Penyegar

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Biology and biotechnological aspect of sweet potato (Ipomoea batatas L.): a commercially important tuber crop

Behera

Chauhan

Pati

et al. 2022

Planta

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Induction of Somatic Embryogenesis and Organogenesis in Zimbabwean Sweet Potato (cv Brondal)

Masekesa

Gasura

Kujeke

et al. 2021

Advances in Agriculture

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Somatic embryogenesis (SE) and organogenesis are crucial in the development of disease free plants and genetic engineering. An investigation was conducted on the ability of treatments containing a combination of 2,4-D and Kinetin to induce either SE or organogenesis from cultured sweet potato cv Brondal leaves. Ten treatments were evaluated and each treatment had an exclusive combination of 2,4-D (at 0.05, 0.1, 0.2, 0.5 or 1 mg/L) to kinetin (at either 0.1 or 0.5 mg/L). Callus initiation occurred earlier in treatments containing higher hormonal concentrations. The 2,4-D to Kinetin ratio had a highly significant ( p = 0.001 ) effect on callus growth and proliferation. Increasing 2,4-D to Kinetin ratio promoted profuse explant callusing while increasing Kinetin to 2,4-D ratio suppressed callusing but encouraged organogenesis, in particular shoot production (treatment containing 0.05 mg/L 2,4-D and 0.5 mg/L Kinetin). Embryogenic calli were formed seven weeks after leaf culture in the treatment containing 0.5 mg/L 2,4-D and 0.1 mg/L Kinetin. The embryogenic calli that developed from this treatment emerged from previously nonembryogenic calli. Plantlets produced via the SE pathway proved to be weak and unviable and died within four weeks of germination. In contrast, plantlets produced under organogenesis were strong, grew vigorously, and could be subcultured several times. This disparity may be accounted for by the fact that the cv Brondal embryos that developed under SE were not exposed to an embryo maturation staged before plantlet germination was initiated. The maturation stage would have assisted embryos to reach physiological maturity and a desired level of desiccation, both being critical elements in embryo to plantlet conversion. In this experiment, cv Brondal regeneration from leaf explants was successfully achieved via organogenesis using 0.05 mg/L 2,4-D and 0.5 mg/L Kinetin, and tentative steps towards development of SE based regeneration protocol were established using 0.5 mg/L 2,4-D and 0.1 mg/L Kinetin.

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Induction of somatic embryogenesis in recalcitrant sweetpotato (Ipomoea batatas L.) cultivars

Cited by 5 publications

References 21 publications

Pengaruh Sitokinin, Jenis Eksplan, dan Genotipe terhadap Embriogenesis Somatik Kakao

Pengaruh Sitokinin, Jenis Eksplan, dan Genotipe terhadap Embriogenesis Somatik Kakao

Biology and biotechnological aspect of sweet potato (Ipomoea batatas L.): a commercially important tuber crop

Induction of Somatic Embryogenesis and Organogenesis in Zimbabwean Sweet Potato (cv Brondal)

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