One Anastomosis Gastric Bypass Reconstitutes the Appropriate Profile of Serum Amino Acids in Patients with Morbid Obesity

Abstract

Bariatric surgery leads to metabolic benefits in patients with obesity, but their mechanisms are not well understood. The appropriate composition of serum amino acids (AA) is important for sufficient supply of these components into various tissues and organs. Obesity leads to alterations in serum AA concentrations. The aim of this study was to examine the effect of one anastomosis gastric bypass (OAGB), a promising type of bariatric surgery, on serum AA concentrations, which were assayed by LC-MS in serum of 46 bariatric patients prior to and 6–9 months after OAGB, as well as in 30 lean control subjects. The results were analyzed by principle components analysis and metabolic pathway analysis. PCA analysis showed that OAGB led to normalization of serum AA concentrations of patients with obesity to a pattern similar to the control subjects, and the concentrations of essential AA remained decreased after OAGB. Changes of individual AA and their associated metabolic pathways were also presented. OAGB caused normalization of the AA profile, which may contribute to improvement of glucose homeostasis and reduction of cardiovascular risk. Considering decreased essential AA concentrations after OAGB, increased intake of high protein food should be recommended to the patients after this type of bariatric surgery.

The Effects of One-Anastomosis Gastric Bypass on Fatty Acids in the Serum of Patients with Morbid Obesity

“…Despite improvements in the composition of some pivotal bioactive FAs, the deregulation of FA profiles associated with obesity seems to persist in patients following OAGB treatment for 6-9 months after the surgery, as evidenced by the grouping of pre-and post-OAGB groups (Figure 1). This observation was dissimilar to the trend to normalization that we previously reported for serum amino acids [9]. We did, however, observe significant changes in some individual FAs or groups of FAs.…”

“…Abbreviations: BMI, body mass index; CRP, C-reactive protein; HbA1C, glycated haemoglobin; HDL, high-density lipoprotein cholesterol; HOMA, homeostatic model assessment of insulin resistance; LC, lean controls; LDL, low-density lipoprotein cholesterol; TG, triacylglycerols; TC, total cholesterol. These data were also presented in our previous study performed on the same group of subjects[9]…”

“…saturated FAs (SFAs) [10], every change in their levels may have an important effect on the metabolic outcome of a bariatric surgery. Recently, in our work, we showed that OAGB reconstitutes the appropriate serum amino acid profiles to the levels measured in nonobese, healthy people [9]. The primary aim of this study is to verify if OAGB also reconstitutes the appropriate serum fatty acid profiles to the levels similar to lean, healthy people.…”

“…T2DM, arterial hypertension, joint disease, or sleep apnoea [7]. An improvement in dyslipidaemia following bariatric treatment is also confirmed in the studies on OAGB [8,9]. However, limiting the investigation of lipid changes to cholesterol and lipoprotein profiles leaves out a multitude of data.…”

Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations

“…Finally, the decrease of the ketogenic amino acid lysine in RYGB feces and increased 3-hydroxybutyrate in RYGB plasma indicates that lysine could be transformed into 3hydroxybutyrate. In this context, it is important to note that increased 3-hydroxybutyrate has also been found in humans after one-anastomosis gastric bypass surgery [72].…”