On the Growth and Survival of <i>Clostridium septicum</i> in Soil

García, Manuel; McKay, K. A.

doi:10.1111/j.1365-2672.1969.tb00985.x

Cited by 8 publications

(5 citation statements)

References 14 publications

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“…The degree of danger posed by C1. perfringens to animal and human health may be determined by the length of time after defaecation that the organism survives in the soil as vegetative cells or as spores (Garcia & Mackay 1969;Collee et al 1971). This merits further investigation.…”

Section: Discussionmentioning

confidence: 99%

Examination of bovine faeces for the isolation and identification of Clostridium species

Princewell

Agba

1982

Journal of Applied Bacteriology

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A total of 162 faecal samples was collected from individual cattle on ten farms from three states of Nigeria. These samples yielded 333 clostridial isolates out of which 295 (88.6%) were identified; the remaining 38 (11.4%) could not be readily identified at species level. The species most frequently isolated was Clostridium perfringens representing 63.7% of the isolates and the next highest was Cl. bifermentans with a frequency of 12.6%. The other species identified were less frequently isolated. No farm yielded all the species isolated. The farm with the highest distribution of the species of Clostridium was the Livestock Investigation Centre, Vom, Plateau State, with a frequency distribution of 62% of the species isolated. In none of the other farms was the distribution up to 50% frequency.

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Section: Discussionmentioning

confidence: 99%

Examination of bovine faeces for the isolation and identification of Clostridium species

Princewell

Agba

1982

Journal of Applied Bacteriology

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“…Clostridial spores can survive in soil for years with little reduction in viable numbers, but less is known about vegetative cells, especially from psychrotolerant strains (Girardin et al, 2005). One of the few studies found that vegetative cells of pathogenic Clostridium septicum survived for 30 days in a range of soils (Garcia & McKay, 1969). Even taking into account extremes and variation in microclimates there is potential for soil temperature, in cooler regions such as the South Island of New Zealand, to remain suitable for the survival of Cl.…”

Section: Discussionmentioning

confidence: 98%

Reduction of spoilage of chilled vacuum-packed lamb by psychrotolerant clostridia

2013

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“…tetani develop from a spore inoculum and persist for up to 9 days in the muscles of experimentally infected guinea-pigs and that virtually no heat-resistant spores seemed to be present in such lesions. The concept of the " significantly viable particle " (Collee et aZ., 1971) may also be valid in the case of natural infection, if the work of Garcia and McKay (1969) on the persistence of CZ. septicum in soil is applicable to wound infection.…”

Section: Discussionmentioning

confidence: 99%

The Recovery Of Clinically Important Anaerobes On Solid Media

Watt

1972

Journal of Medical Microbiology

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IT was shown by Collee, Rutter and Watt (1971) that the difficulties encountered in the surface culture of Clostridium oedematiens (CZ. novyi) types B and D can be overcome by the use of modified Moore's medium (Moore, 1968) or by sprinkling sterile iron filings on the surface of a seeded blood agar plate. This approach made it possible to study quantitatively whether the growth was derived from spores or from vegetative cells. It was found for type D of this exacting organism that vegetative cells can account for an appreciable part of the growth obtained when a broth culture is subcultured on solid medium. This finding may be of practical importance also in the case of other anaerobes. It raises the question whether the traditional methods give adequate recovery of anaerobic organisms for clinical purposes or whether more elaborate procedures such as those of Hungate (1950) The following organisms were subcultured in nutrient broth after isolation from clinical material and identification : two strains of Staphylococcus aureus; one strain of Streptococcus pyogenes (group A), one strain of Bacillus anthracoides; one strain of Neisseria gonorrhoeae ; one strain of Veillonella sp.; and two strains of Haernophilus influenzae.Media. Cooked-meat broth was prepared as described by Cruickshank (1968, p. 757), but the infusion broth component was made from Nutrient Broth no. 2 (Oxoid). Oxoid Nutrient Broth was also used for the subculture of some of the test organisms. "Presteamed nutrient broth " was used as a diluent in the quantitative studies; it was heated by steaming at 100°C for 30 min. to remove dissolved air, then cooled rapidly to 37°C.In the initial studies, Watt's modification of Moore's medium (cysteine-dithiothreitol human blood agar) was used (Collee et ~1 . ) . In later studies, a further modification (cysteinedithiothreitol equine blood agar was prepared by substituting 10 per cent. horse blood for 33 per cent. human blood.Blood agar plates were prepared with Oxoid Blood Agar Base no. 2 enriched with equine blood (10 per cent.) or human blood (33 per cent.).Blood. Outdated citrated human blood was obtained from the Blood Transfusion Department, Edinburgh Royal Infirmary. Defibrinated equine blood was supplied by Wellcome Laboratories.

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On the Growth and Survival of Clostridium septicum in Soil

Cited by 8 publications

References 14 publications

Examination of bovine faeces for the isolation and identification of Clostridium species

Examination of bovine faeces for the isolation and identification of Clostridium species

Reduction of spoilage of chilled vacuum-packed lamb by psychrotolerant clostridia

The Recovery Of Clinically Important Anaerobes On Solid Media

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