Olive tree in vitro establishment under different culture media and explant collection periods

Abstract

In the species Olea europaea L. the tissue culture techniques have not been sufficiently studied by the lack of efficient establishment of protocols for various cultivars. The objective of this work was to evaluate the effect of different culture media and sampling times of explants on in vitro establishment of six cultivars of olive (Ascolano 315, Leccino, Maria da Fé, Coratina, Arbequina and Frantoio). The work was divided into two experiments conducted in the Fruit Tree Propagation Laboratory, Crop Science Department, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Brazil. Stock plants of the olive cultivars maintained in semi- hydroponic system were used. In the first experiment, nodal segments were used to establish in vitro culture medium MO and WPM, consisting of salts and vitamins. In the second experiment four seasons to obtain the explants were evaluate (autumn, winter, spring and summer). The WPM medium promoted greater establishment of olive explants. There is a higher phenolic oxidation in olive explants collected in winter. Spring is indicated for collecting explants, therefore favors the in vitro cultivar Maria da Fé, while Ascolano 315 and Arbequina cultivars have a higher rate of in vitro establishment by collecting explants over the year

Season, basal media and plant growth regulators effect in wood plant in vitro propagation: a comprehensive review

“…The pH factor of the base medium is also important because it affects both plant growth and the activity of growth regulators. The composition of the basic medium and its combination with plant hormones and nitrogen sources has a profound effect on the beginning of the explanatory response [26].…”

“…Each type is preferred for various aspects in the culture of plants. Identification of different culture media [26], plant tissue culture media and practices [35], Effect various plant growth regulator [34] and combination effect PGRs, Season and type cutting explant [9] the results have been made available.…”

“…Successful propagation of tissue cultures of woody plants has been widely reported, among others, in the species Tectona grandis [34], Santalum album [29], Acacia sinuate [18] and Aquilaria malaccensis Lam [30]. Separate screenings related to the influence of seasons [3,5,6,8,21] basic media [16,35] and growth-stimulating substances [13,14,34] have been widely reported as well as the results of comprehensive screening related to the three factors mentioned above [1,2,4,9,12,13,18,20,22,23,[25][26][27][28][29][30].…”

Rooting dynamics of Prunus minicuttings

“…Among the vegetative propagation processes, minicutting is an improvement of cutting technique and has been used in the multiplication of several plant species, such as eucalyptus (Eucalyptus benthamii) (Brondani, Baccarin, Ondas, Gonçalves, & Almeida, 2012), Australian cedar (Toona ciliata) (Silva, Barroso, Souza, Ferreira, & Carneiro, 2012), and also in several fruit trees, such as guava tree (Psidium guajava) (Marinho, Milhem, Altoé, Barroso, & Pommer, 2009;Altoé & Marinho, 2012), araçá (Psidium cattleianum) (Altoé, Marinho, Terra, & Barroso, 2011), soursop (Annona muricata) (Figueirêdo, Vilasboas, Oliveira, Sodré, & Sacramento, 2013), blueberry tree (Vaccinium spp.) (Fischer, Fachinello, Antunes, Fischer, & Giacobbo, 2013;Yamamoto et al, 2017;Koyama et al, 2018), peach tree (Prunus persica) (Timm, Schuch, Tomaz & Mayer, 2015a;Tomaz, Schuch, Peil, & Timm, 2014;Tomaz, Schuch, Peil, & Pereira, 2016;Ramm, Schuch, Fagundes, Silva, & Moreira, 2017), cherry (Ozelame, Affonso, Cappellaro, Schuch, &Tomaz, 2018, blackberry andraspberry (Sommer et al, 2016;Fagundes et al, 2016), and more recently the olive tree (Olea europaea) (Casarin, Moreira, Raasch, Timm, & Schuch, 2018;Moreira & Schuch, 2018).…”