Offspring from Heterotopic Transplantation of Newborn Mice Ovaries

Abstract

This study is aimed at investigating the developmental potential of the primordial follicles from ovaries of newborn mice after cryopreservation in liquid nitrogen for long-term storage, thawing, and heterografting into the kidney capsules of ovariectomized adult female mice. After stimulation of recipient mice with pregnant mare serum gonadotropin on day-19 after heterografting, the primordial follicles of the transplanted ovaries could develop into antral follicles. When the oocyte-cumulus cell complexes were retrieved from these antral follicles, they could mature after in vitro culture for 16–17 h. After in vitro fertilization, the rates of embryos derived from these oocytes that developed into the two-cell stage and the blastocyst stage after 16–17 h and after day-4, respectively,in the culture medium were 55.40% (55/107) and 9.09% (5/55),respectively. In the ovarian transplantation groups, no pups were derived from the 410 embryos that were transferred into 10 pseudopregnant mothers at the pronuclear stage. However,of the 10 surrogate mothers in whom 570 embryos were transferred at the two-cell stage, four achieved pregnancy and gave birth to 20 live offspring. These results demonstrated that primordial follicles in newborn mice ovaries were capable of sustaining their developmental potential after freezing and thawing. Once transplanted into the kidney capsules of ovariectomized adult female mice, these primordial follicles could develop and respond to gonadotropin stimulation and reach the antral stage; further, live offspring could be derived from these follicles.

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

“…animals, including sheep [1], goats [2,3], primates [4], rodents [5], and humans [6]. Satisfactory results have been reported, especially when a 1.5 M concentration was used in slow-freezing protocols.…”

Current and Future Perspectives for Improving Ovarian Tissue Cryopreservation and Transplantation Outcomes for Cancer Patients

“…Heterotopic transplantation is considered a rarely applied method, as multiple physical and biological requirements related to OT are not met via this approach. In animal studies, offspring are reportedly obtained from heterotopic transplants placed close to the cutaneous area [ 113 , 114 ]. Additionally, oocytes and embryos have been obtained in humans as a result of subcutaneous transplantation [ 115 ].…”

Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice

“…Advances in cancer therapy have improved survival outcomes in patients with cancer. However, reduced fertility, with premature ovarian failure, is often observed in young women following treatment for cancer [ 1 , 2 ]. Several options, such as oocyte/embryo or ovarian tissue (OT) cryopreservation [ 3 ], are available for fertility preservation in such patients.…”