Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice

Kong, Hyun Sun; Lee, Jaewang; Youm, Hye Won; Kim, Seul Ki; Lee, Jung Ryeol; Suh, Chang Suk; Kim, Seok Hyun

doi:10.1371/journal.pone.0184546

Cited by 26 publications

(23 citation statements)

References 50 publications

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“…In the present study, the deleterious effect of VEGF on developing follicles, follicular and stromal cell and vessel densities, and collagen type I was more likely to be due to the prolonged exposure time (24 h) to VEGF. This assumption is supported by the following findings: (i) the exposure of fragments to VEGF at room temperature for a short period (30 min) did not affect the studied end points, except for a higher number of AgNORs, and (ii) the dose of VEGF herein used has not revealed any toxic effect in studies in vitro (goats [ 70 ] and cattle [ 75 ]) and in vivo (mice [ 72 ], rats [ 72 ], rabbits [ 29 ], cattle [ 76 ], and humans [ 37 ]). In regard to angiogenesis, an improper balance between stimulators and inhibitors can lead to excessive angiogenic signaling and later pathological processes, including intense endothelial permeability that leads to vasogenic edema, inflammatory process, apoptosis, and cell death [ 77 – 80 ].…”

Section: Discussionmentioning

confidence: 99%

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Souza

Alves

et al. 2020

PLoS ONE

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Heterotopic and orthotopic ovarian tissue autotransplantation techniques, currently used in humans, will become promising alternative methods for fertility preservation in domestic and wild animals. Thus, this study describes for the first time the efficiency of a heterotopic ovarian tissue autotransplantation technique in a large livestock species (i.e., horses) after ovarian fragments were exposed or not to a cooling process (4°C/24 h) and/or VEGF before grafting. Ovarian fragments were collected in vivo via an ultrasound-guided biopsy pick-up method and surgically autografted in a subcutaneous site in both sides of the neck in each mare. The blood flow perfusion at the transplantation site was monitored at days 2, 4, 6, and 7 post-grafting using color-Doppler ultrasonography. Ovarian grafts were recovered 7 days post-transplantation and subjected to histological analyses. The exposure of the ovarian fragments to VEGF before grafting was not beneficial to the quality of the tissue; however, the cooling process of the fragments reduced the acute hyperemia post-grafting. Cooled grafts compared with non-cooled grafts contained similar values for normal and developing preantral follicles, vessel density, and stromal cell apoptosis; lower collagen type III fibers and follicular density; and higher stromal cell density, AgNOR, and collagen type I fibers. In conclusion, VEGF exposure before autotransplantation did not improve the quality of grafted tissues. However, cooling ovarian tissue for at least 24 h before grafting can be beneficial because satisfactory rates of follicle survival and development, stromal cell survival and proliferation, as well as vessel density, were obtained.

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Section: Discussionmentioning

confidence: 99%

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Souza

Alves

et al. 2020

PLoS ONE

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“…Thus, in cold and hypoxic environments, it will be supposed that larger arteriole branches diameter are beneficial for transportation of more red cells, oxygen supply and gas exchange. As an alternative mechanism, Ang‐2 increases the sensitivity of endothelial cells to VEGF and promotes angiogenesis and vessel growth by increasing expression of VEGF (Kong et al, 2017). In present study, gene expression level of Ang‐2 was the lowest in ovaries of Small‐tailed Han sheep, while it was the highest in Tibetan sheep.…”

Section: Discussionmentioning

confidence: 99%

Vascular morphology and expression of angiogenic factors in sheep ovaries and their relation with the lambing rate

Wang

Yang

2020

Reprod Domestic Animals

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The study aims to present the vascular anatomy of ovarian arterial system in sheep and to explore its relation with the angiogenic factors vascular endothelial growth factor (VEGF) and angiopoietin‐2 (Ang‐2), environmental factors and the lambing rate. Small‐tailed Han sheep, Hu sheep, Aohan finewool sheep and Tibetan sheep who are from different habitats in China and with different lambing rates were investigated. The vascular corrosion casting technique and scanning electron microscopy were used to understand arterial architecture, and real‐time quantitative PCR (qRT‐PCR) and Western blotting were used for assessing VEGF and Ang‐2 expression. Results showed that high‐prolificity breeds had significantly larger vessel diameters in ovaries than low‐prolificity ones. VEGF gene transcript and protein expression were related with the lambing rate, while Ang‐2 gene transcript was negatively related with the lambing rate. Results indicated that high‐prolificity breeds have more abundant arterial blood supply, which is associated with VEGF expression.

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“…As a result, the transplanted ovarian tissue is exposed for a certain time period of 5 days 82 in a hypoxic and ischemic environment which demands an urgent intervention to induce neovascularization. In this context, various approaches have been attempted to optimize the outcome including employment of growth factors as a supplementary tool to assist toward completion of neovascularization in a timely fashion 83 . However, their application in human models is nowhere near consideration even though current experimentation on animals has contributed favorable results.…”

Section: Ovarian Tissue Transplantationmentioning

confidence: 99%

Novel Approaches in Addressing Ovarian Insufficiency in 2019: Are We There Yet?

Sfakianoudis¹,

Rapani

Grigoriadis

et al. 2020

Cell Transplant

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Ovarian insufficiency is described as a multifaceted issue typically encountered in the field of assisted reproduction. The three main identified diagnoses of ovarian insufficiency include premature ovarian failure (POF), poor ovarian response (POR), and advanced maternal age (AMA). Patient heterogeneity in the era of individualized medicine drives research forward leading to the emergence of novel approaches. This plethora of innovative treatments in the service of adequately managing ovarian insufficiency is called to undertake the challenge of addressing infertile patients exploring their reproductive options. This review provides an all-inclusive presentation and critical analysis on novel treatments that have not achieved routine clinical practice status yet, but have recently emerged as promising. In light of the lack of randomized controlled trials conveying safety and efficiency, clinicians are left puzzled in addressing the “how” and “for whom” these approaches may be beneficial. From ovarian injection employing platelet-rich plasma (PRP) or stem cells to artificial gametes and ovaries, ovarian transplantation, and mitochondrial replacement therapy, this descriptive review provides insight toward assisting the practitioner in decision making regarding these cutting-edge treatments. Biological mechanisms, invasiveness levels, efficiency, as well as possible complications, the current status along with bioethical concerns are discussed in the context of identifying future optimal treatment.

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Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice

Cited by 26 publications

References 50 publications

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Vascular morphology and expression of angiogenic factors in sheep ovaries and their relation with the lambing rate

Novel Approaches in Addressing Ovarian Insufficiency in 2019: Are We There Yet?

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