Occurrence of Extended-Spectrum β-Lactamases in Members of the Genus
            <i>Shigella</i>
            in the Republic of Korea

Kim, Seonghan; Kim, Junyoung; Kang, Yeon‐Ho; Park, YongKeun; Lee, Bok-Kwon

doi:10.1128/jcm.42.11.5264-5269.2004

Cited by 67 publications

(49 citation statements)

References 23 publications

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“…Since that time many strains of Shigella spp. harbouring different types of ESBLs have been reported in developed and developing countries in Asia (Cheung et al, 2005;Chuang et al, 2006;Kim et al, 2004;Pai et al, 2001;Rahman et al, 2004;Xiong et al, 2007). However, to the best of our knowledge this is the first report of ESBLproducing Shigella spp.…”

Section: Rapid Emergence Of Third Generation Cephalosporin Resistant mentioning

confidence: 49%

Rapid emergence of third generation cephalosporin resistant Shigella spp. in Southern Vietnam

Vinh

Baker

Campbell

et al. 2009

Journal of Medical Microbiology

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Section: Rapid Emergence Of Third Generation Cephalosporin Resistant mentioning

confidence: 49%

Rapid emergence of third generation cephalosporin resistant Shigella spp. in Southern Vietnam

Vinh

Baker

Campbell

et al. 2009

Journal of Medical Microbiology

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“…12 The ESBL positive donor strains were conjugated with the standard strain E. coli J53 (resistant to sodium azide) as a recipient cell. MacConkey agar containing 4 µg/mL of cefotaxime and 200 µg/mL of sodium azide was used as the selection medium.…”

Section: Conjugation Assay For Esbl Positive Strainsmentioning

confidence: 99%

“…The conjugation assay was performed using a modified method described by Kim et al (2004). 12 The ESBL positive donor strains were conjugated with the standard strain E. coli J53 (resistant to sodium azide) as a recipient cell.…”

Section: Conjugation Assay For Esbl Positive Strainsmentioning

confidence: 99%

Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of <i>Proteus mirabilis</i> from SouthWest Nigeria

et al. 2017

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Background: Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase in bacterial resistance has been phenotypically established but due to high cost, few molecular studies have been reported. Objectives: This study screened for presence of transferable resistance genes and mobile genetic elements (MGEs) such as integron among multi-drug resistant (MDR) P. mirabilis. Methods: A total of 108 P. mirabilis strains collected from five tertiary hospitals in SouthWest Nigeria were subjected to antibiotic susceptibility study using disc-diffusion method. Transferable resistance genes and MGEs were amplified using Polymerase chain reaction (PCR) analysis and amplicons sequenced. Results: Varied resistance was observed against all the antibiotics tested. About 56% of the isolates were MDR including those from 0-12 years old children. PCR analysis revealed the presence of aac(6')-Ib (33.3%), plasmid mediated quinolone resistance (PMQR) genes [qnrA (36.7%), acc(6')-Ib-cr (5%)], TEM (48.3%), CTX-M (6.7%) and integrons class 1 (58.3%) and class 2 (26.7%). Sequencing analysis revealed bla TEM-1 , bla CTX-M-15 associated with ISEcp1 and eight different arrays of gene cassettes: aadA1, aadA1-qacH, aadB-aadA2, aadA5, dfrA7, dfrA15, dfrA17, dfrA17-aadA5. Conclusion: Transferable resistance genes in association with MGEs are present in Nigerian P. mirabilis thus their potential in disseminating resistance.

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“…These have the semblances of the results obtained by Kim et al (2004) in which plasmid presence and transfer of ESBL phenotype to E. coli was successful for all their 20 isolates. Also, as in ours, the electrophoresis of their plasmid DNA from wild-type Shigella spp.…”

Section: Discussionmentioning

confidence: 95%

Drug resistance pattern of Salmonella and Shigella species isolated from selected hospitals in Anyigba, Kogi State, Nigeria

Ocean¹,

Eze²,

Eze³

et al. 2015

Afr. J. Microbiol. Res.

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This work was designed to assess the status of Salmonella and Shigella infections in Anyigba, Kogi State, Nigeria with a view to making informed statements that may guide policy makers and public health experts in the containment efforts against these infections. Salmonella and Shigella species isolated from human faecal samples were examined for resistance to some antibiotic agents using disc diffusion methods. Drug resistant isolates were analyzed for presence of plasmids by basic curing and transferability methods. Age distribution of sources of resistant isolates showed that Shigella isolates from Youths (18 to 30 years) were more resistant to Ampicilin (66.67%) and Augmentin (33.33%) than isolates from Infants (6 months to 4 years) (3% Ampicillin and 0% Augmentin). Salmonella species isolated from Adults (31 and above) were more resistant to Augmentin (45%) and Ampicillin (40%), than isolates from Youths (18 to 31 years) (40% Ampicillin and 36.67% Augmentin). Salmonella isolated from Infants (6 months to 4 years) showed percentage resistance of 28.57% to Ampicillin and 14.29% to Augmentin while isolates from Children (5 to 17 years) showed percentage resistance of 25% to Ampicillin and 12.5% to Augmentin. Salmonella species showed higher percentage resistance to commonly used drugs than Shigella species. The result shows that 8% of Shigella species and 21.5% of Salmonella species were resistant to more than eight antibiotics with (MAR) index of between 0.2-0.9. Results of curing and agarose gel electrophoresis (AGE) presumptively showed that the resistance traits were plasmid borne. AGE showed plasmids of molecular weights 23.1 kb for both species of Salmonella and Shigella suggesting their possible exchange among them. This was substantiated by conjugative transfer of resistance determinants among few isolates. The results show convincing evidence of the presence of multidrug resistant Salmonella and Shigella spp. within the study population.

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Occurrence of Extended-Spectrum β-Lactamases in Members of the Genus Shigella in the Republic of Korea

Cited by 67 publications

References 23 publications

Rapid emergence of third generation cephalosporin resistant Shigella spp. in Southern Vietnam

Rapid emergence of third generation cephalosporin resistant Shigella spp. in Southern Vietnam

Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of <i>Proteus mirabilis</i> from SouthWest Nigeria

Drug resistance pattern of Salmonella and Shigella species isolated from selected hospitals in Anyigba, Kogi State, Nigeria

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