Occurrence of a
 Salmonella enterica
 Serovar Typhimurium DT104-Like Antibiotic Resistance Gene Cluster Including the
 floR
 Gene in
 S. enterica
 Serovar Agona

Cloeckaert, Axel; Sidi-Boumedine, Karim; Flaujac, Geraldine; Imberechts, Hein; D'hooghe, I.; Chaslus-Dancla, Élisabeth

doi:10.1128/aac.44.5.1359-1361.2000

Cited by 82 publications

(89 citation statements)

References 13 publications

(14 reference statements)

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“…Thus irrespective of source and country of origin the investigated R-type ACSSuSpT strains contained the same inserted gene cassettes. In addition to these findings, other investigations [9,10] however showed that the chromosomally located antibiotic resistance genes also occur in other phage-types rather than DT104 and more interestingly also in another Salmonella serovar, namely Salmonella Agona. This could be indicative of horizontal transfer of the antibiotic resistance gene cluster, perhaps phage-mediated.…”

Section: Horizontal Gene Transfer Versus Clonal Spreadmentioning

confidence: 74%

Molecular tools for the characterisation of antibiotic-resistant bacteria

Aarts¹,

Sidi-Boumedine

Nesme

et al. 2001

Vet. Res.

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-This review will discuss a number of molecular tools which are currently used as well as some innovative approaches for the characterisation of antibiotic-resistant bacterial strains. Various methods involved in the detection and characterisation of genes and mutations associated with antibiotic resistance and that are used for strain typing as part of epidemiological studies, are described. Furthermore, a few examples are discussed in which the results of both gene and strain characterisation are combined to investigate the underlying mechanism of the spread of antibiotic resistance. Some of the available molecular techniques are heavily supported by the existence of databases on the Internet. These databases either contain a fast growing amount of sequence information or a large number of allelic or fingerprint profiles. The current progress in applied DNA technology and the ongoing projects on the elucidation of the whole genomic sequence of bacterial species have lead and will further lead to the development and application of sophisticated new strategies for the analysis of antibiotic resistant bacterial strains.antibiotic resistance / molecular detection / molecular typing / horizontal gene transfer / new approaches Résumé -Outils moléculaires pour la caractérisation de bactéries résistantes aux antibiotiques. Cette revue présente les outils moléculaires utilisés actuellement ainsi que des nouvelles approches pour la caractérisation de souches bactériennes résistantes aux antibiotiques. Différentes méthodes de détection et de caractérisation des gènes et des mutations impliqués dans la résistance aux antibiotiques, utilisées dans des études épidémiologiques, sont décrites. Quelques exemples sont présentés où sont associées les caractérisations du gène et de la souche bactérienne dans l'étude des mécanismes de diffusion de résistances aux antibiotiques. Certaines techniques moléculaires font

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Section: Horizontal Gene Transfer Versus Clonal Spreadmentioning

confidence: 74%

Molecular tools for the characterisation of antibiotic-resistant bacteria

Aarts¹,

Sidi-Boumedine

Nesme

et al. 2001

Vet. Res.

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“…These multidrug-resistant serovar Agona strains were isolated from poultry in Belgium during the 1992-1997 period [24], but have not yet been reported in other countries. Besides this observation, the DT104 antibiotic resistance gene cluster has also been shown to occur in phage type DT120 of serovar Typhimurium [18]. A possible mechanism by which this antibiotic resistance gene cluster may be horizontally transferred is phage-mediated transfer.…”

Section: Clonal Spread and Horizontal Transfer Of The Antibiotic Resimentioning

confidence: 81%

“…Multiplex PCR based on the surrounding genes of the antibiotic resistance gene cluster has also been proposed for the identification of S. enterica Typhimurium DT104 strains [5,12,26]. In the light of evidence of horizontal transfer of the DT104 antibiotic resistance gene cluster to other Typhimurium phage types or other S. enterica serovars [18], these methods appear to be no longer suitable for the specific detection of multidrug-resistant S. enterica Typhimurium DT104. Let us also mention the recently described occurrence of florfenicol resistance encoded by the floR gene on conjugative plasmids or on the chromosome of Escherichia coli isolated from cattle and poultry [17,25,53].…”

Section: Molecular Identification and Detection Methodsmentioning

confidence: 99%

“…The best evidence for horizontal transfer of the antibiotic resistance gene cluster is the recent discovery of this cluster in another S. enterica serovar, namely serovar Agona [18]. These multidrug-resistant serovar Agona strains were isolated from poultry in Belgium during the 1992-1997 period [24], but have not yet been reported in other countries.…”

Section: Clonal Spread and Horizontal Transfer Of The Antibiotic Resimentioning

confidence: 99%

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Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

2001

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-Multidrug-resistant Salmonella enterica serovar Typhimurium phage type DT104 has emerged during the last decade as a global health problem because of its involvement in diseases in animals and humans. Multidrug-resistant DT104 strains are mostly resistant to ampicillin, chloramphenicol, streptomycin, sulfonamides and tetracyclines (ACSSuT resistance type). The genes coding for such resistances are clustered on the chromosome. This paper reviews new developments in the characterization of S. enterica Typhimurium DT104, its chromosomal antibiotic resistance genes and their spread among other S. enterica Typhimurium phage types and other S. enterica serovars, the development of specific detection methods, virulence characteristics, and the evolution of multidrugresistance with regard to the emergence of quinolone resistance.Salmonella Typhimurium DT104 / multidrug resistance / Salmonella genomic island I Résumé -Caractérisation moléculaire, diffusion et évolution de la résistance multiple aux antibiotiques chez Salmonella enterica Typhimurium DT104. Le lysotype DT104 de Salmonella enterica Typhimurium présentant une résistance multiple aux antibiotiques a émergé durant la dernière décennie comme un problème mondial de santé publique qui touche à la fois humains et animaux. Les souches du lysotype DT104 sont en général résistantes à l'ampicilline, au chloramphénicol, à la streptomycine, aux sulphamides et aux tétracyclines (profil de résistance ACSSuT). Les gènes codant pour ces résistances sont groupés sur le chromosome. Cet article présente une revue des derniers dévelop-pements dans la caractérisation de S. enterica Typhimurium DT104, ses gènes chromosomiques de résis-tance et leur diffusion chez d'autres lysotypes de S. enterica Typhimurium et d'autres serotypes de S. enterica, le développement de méthodes spécifiques de détection, les caractéristiques de virulence, et l'évolution de la résistance multiple au regard de l'émergence de la résistance aux quinolones.Salmonella Typhimurium DT104 / résistance multiple / îlot génomique I Vet. Res. 32 (2001) [301][302][303][304][305][306][307][308][309][310] 301

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“…Efflux systems which confer either only chloramphenicol resistance or combined resistance to chloramphenicol and florfenicol have also been described [2,3,8,17,18,32,77]. Permeability barriers and multidrug transporters only play a role in certain gram-negative bacteria [44,48].…”

Section: Resistance To Chloramphenicol and Florfenicolmentioning

confidence: 99%

Use of antimicrobials in veterinary medicine and mechanisms of resistance

2001

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-This review deals with the application of antimicrobial agents in veterinary medicine and food animal production and the possible consequences arising from the widespread and multipurpose use of antimicrobials. The various mechanisms that bacteria have developed to escape the inhibitory effects of the antimicrobials most frequently used in the veterinary field are reported in detail. Resistance of bacteria to tetracyclines, macrolide-lincosamide-streptogramin antibiotics, β-lactam antibiotics, aminoglycosides, sulfonamides, trimethoprim, fluoroquinolones and chloramphenicol/florfenicol is described with regard to enzymatic inactivation, decreased intracellular drug accumulation and modification/protection/replacement of the target sites. In addition, basic information is given about mobile genetic elements which carry the respective resistance genes, such as plasmids, transposons, and gene cassettes/integrons, and their ways of spreading via conjugation, mobilisation, transduction, and transformation.antibiotic therapy / growth promotion / resistance mechanism / resistance gene / gene transfer Résumé -Utilisation d'agents antimicrobiens en médecine vétérinaire et mécanismes de résis-tance. Cette revue présente les différents buts pour lesquels les agents antimicrobiens sont utilisés en médecine vétérinaire, dans les élevages d'animaux entrant dans la chaîne alimentaire et les possibles conséquences de cette large utilisation. Une synthèse est faite des différents mécanismes de résis-tance développés par les bactéries, comme l'inactivation enzymatique, la diminution de la concentration intracellulaire de l'antibiotique, les modification/protection/déplacement de cible, qui permettent d'échapper à l'action des antibiotiques les plus fréquemment utilisés dans le domaine vétérinaire :

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Occurrence of a Salmonella enterica Serovar Typhimurium DT104-Like Antibiotic Resistance Gene Cluster Including the floR Gene in S. enterica Serovar Agona

Cited by 82 publications

References 13 publications

Molecular tools for the characterisation of antibiotic-resistant bacteria

Molecular tools for the characterisation of antibiotic-resistant bacteria

Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

Use of antimicrobials in veterinary medicine and mechanisms of resistance

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