2001
DOI: 10.1051/vetres:2001130
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Molecular tools for the characterisation of antibiotic-resistant bacteria

Abstract: -This review will discuss a number of molecular tools which are currently used as well as some innovative approaches for the characterisation of antibiotic-resistant bacterial strains. Various methods involved in the detection and characterisation of genes and mutations associated with antibiotic resistance and that are used for strain typing as part of epidemiological studies, are described. Furthermore, a few examples are discussed in which the results of both gene and strain characterisation are combined to… Show more

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Cited by 22 publications
(13 citation statements)
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“…It is also interesting to note that strains showing the same macrorestriction pattern nevertheless exhibit genetic diversity when using other methods such as infrequent restriction site PCR (IRS-PCR) and amplified fragment length polymorphism (AFLP) [46]. The latter methods may be more discriminatory than PFGE and the usefulness and limits of each method are described by Aarts et al [1] in this special issue. The occurrence of the DT104 antibiotic resistance gene cluster in several clones would suggest horizontal transfer of the cluster and possibly of the entire SgiI among S. enterica Typhimurium strains.…”
Section: Clonal Spread and Horizontal Transfer Of The Antibiotic Resimentioning
confidence: 99%
“…It is also interesting to note that strains showing the same macrorestriction pattern nevertheless exhibit genetic diversity when using other methods such as infrequent restriction site PCR (IRS-PCR) and amplified fragment length polymorphism (AFLP) [46]. The latter methods may be more discriminatory than PFGE and the usefulness and limits of each method are described by Aarts et al [1] in this special issue. The occurrence of the DT104 antibiotic resistance gene cluster in several clones would suggest horizontal transfer of the cluster and possibly of the entire SgiI among S. enterica Typhimurium strains.…”
Section: Clonal Spread and Horizontal Transfer Of The Antibiotic Resimentioning
confidence: 99%
“…Pulsed-field gel electrophoresis (PFGE) allows identification of strains by their molecular genotypes and has great value in epidemiological analysis, in the differentiation of pathogenic strains, and in monitoring the spread of such strains between communities (14,17,24). This method can also be used to evaluate the potential clonal spread of resistant strains (1,44). It has previously been used to investigate the dissemination and diversity of ampicillin-resistant commensal E. coli strains in cattle (22,23) and dogs (32).…”
mentioning
confidence: 99%
“…Therefore, the increasing number of serovar Newport infections appears to be associated with the emergence and rapid dissemination of multidrugresistant (MDR) strains of serovar Newport (18). Multidrug resistance is often associated with mobile genetic elements (i.e., plasmids and transposons) that encode multiple specific resistance genes (1,12,13,17,19).…”
mentioning
confidence: 99%