Occurrence, identification and pathogenicity of Fusarium species associated with bakanae disease of basmati rice in India

Bashyal, Bishnu Maya; Aggarwal, Rashmi; Sharma, Sapna; Gupta, Sangeeta; Rawat, Kirti; Singh, Dhiraj; Singh, A. K.; Krishnan, S. Gopala

doi:10.1007/s10658-015-0783-8

Cited by 46 publications

(44 citation statements)

References 17 publications

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“…were not re-isolated, thus suggesting their possible presence in the form of saprophytic or epiphytic fungi. Similar study has been carried out on basmati rice in India, where F. fujikuroi was found as the most dominant species, and all the 41 isolates of F. fujikuroi resulted pathogenic (29% highly virulent, 34% medium virulent, and 37% moderately virulent (28). Different degrees of virulence were observed among the F. fujikuroi strains from the same cultivation area and from the same cultivars, thus indicating the possible influence of genetic features of the strains, rather than the involvement of environmental factors.…”

Section: Pathogenicity and Monitoring Of Fusarium Fujikuroisupporting

confidence: 68%

The puzzle of bakanae disease through interactions between Fusarium fujikuroi and rice

Spadaro¹

2017

Front Biosci

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Bakanae disease, one of the most noteworthy seedborne rice diseases, is caused by Fusarium fujikuroi, a member of the Gibberella fujikuroi species complex. The decreasing availability of chemical seed-dressing products over the last few years has raised the concerns of rice seed companies regarding bakanae disease. Therefore, new research trends require a deeper investigation into the main aspects of bakanae disease through interactions between rice and F. fujikuroi, in order to find new resistant or tolerant cultivars and alternative bakanae disease control strategies, as well as to develop more sensitive molecular diagnostic techniques. Here, some new aspects of F. fujikuroi epidemiology and pathogenicity, as well as its interactions with rice, are reported, and recent approaches applied to control bakanae disease are summarized.

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Section: Pathogenicity and Monitoring Of Fusarium Fujikuroisupporting

confidence: 68%

The puzzle of bakanae disease through interactions between Fusarium fujikuroi and rice

Spadaro¹

2017

Front Biosci

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“…Among the biotic stresses, insect pests and diseases caused by bacteria, fungi, nematodes and viruses are the major factors affecting the rice production. Among the potentially important diseases of contemporary importance, Bakanae or foot rot disease, caused by Fusarium fujikuroi (Nirenberg), [teleomorph: Gibberella fujikuroi (Sawada) Ito] has emerged as a disease of major concern (Bashyal et al 2016 ). The disease can cause upto 70 % yield loss and impairs the grain quality as well, under the field conditions (Fiyaz et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Mapping quantitative trait loci responsible for resistance to Bakanae disease in rice

Fiyaz

Yadav

Krishnan

et al. 2016

Rice

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BackgroundBakanae or foot rot disease caused by Fusarium fujikuroi [teleomorph: Gibberella fujikuroi (Sawada) Ito] is emerging as a serious disease in rice. The disease causes both quantitative and qualitative losses to the grains under the field conditions. Breeding for resistance to Bakanae disease is a promising strategy to manage this emerging disease. In this study, we used a population of 168 F14 recombinant inbred lines (RILs) derived from two indica rice parents Pusa 1342, a highly resistant variety and Pusa Basmati 1121, a highly susceptible variety to map quantitative trait loci (QTLs) governing resistance against Bakanae disease.ResultsThe disease reaction of 168 F14 RILs were measured on the seedlings inoculated using Fusarium fujikuroi culture using high-throughput screening protocol under glasshouse conditions. Utilizing inclusive composite interval mapping, three QTLs governing resistance to Bakanae were identified, namely qBK1.1, qBK1.2 and qBK1.3 which accounted 4.76, 24.74 and 6.49 % of phenotypic variation, respectively. The major effect QTL designated qBK1.2 was mapped in 0.26 Mb region between RM5336 and RM10153. A total of 55 annotated genes were identified within the identified QTL region qBK1.2.ConclusionsThe novel QTLs identified in this study are useful resource for efficiently breeding rice cultivars resistant to Bakanae disease. This is the first report on identification of QTLs governing resistance against Bakanae in rice using inclusive composite interval mapping strategy in a RIL population.

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“…The F. fujikuroi and F. proliferatum isolates, as well as strains of other Fusarium species, are stored in the Department of Plant Pathology, Nanjing Agricultural University, China. All strains were identified based on their morphology and ITS and tef‐1 α sequences (Bashyal, Aggarwal, & Sharma Gupta, ; Leslie & Summerell, ).…”

Section: Methodsmentioning

confidence: 99%

“…Pathology, Nanjing Agricultural University, China. All strains were identified based on their morphology and ITS and tef-1α sequences (Bashyal, Aggarwal, & Sharma Gupta, 2016;Leslie & Summerell, 2006). filtration and frozen at −20°C.…”

Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of rice bakanae caused by Fusarium fujikuroi and F. proliferatum using loop‐mediated isothermal amplification assays

Rong

Yuan

et al. 2018

Journal of Phytopathology

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Rice bakanae is an important disease that causes serious rice production loss worldwide. We describe a new method for rapid diagnosis of rice bakanae caused by Fusarium fujikuroi and F. proliferatum, based on loop‐mediated isothermal amplification (LAMP) assays. After screening, primers were selected to target FusariumDNA sequences, that is, the intergenic spacer (IGS) region of the nuclear ribosomal operon and reductase‐coding region (RED1) in F. fujikuroi and F. proliferatum, respectively. Both LAMP assays efficiently amplified target genes in 70 min at 62°C. A colour change from purple to sky blue (visible to the unaided eye) was observed in the presence of the DNA of the targeted pathogens only, by adding hydroxynaphthol blue to the reaction system prior to amplification. The minimum of genomic DNA needed in the assays was 67 and 346 pg/μl for F. fujikuroi and F. proliferatum, respectively. Using the two assays described here, we successfully and rapidly diagnosed suspected diseased rice plant and seed samples collected from Jiangsu Province.

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Occurrence, identification and pathogenicity of Fusarium species associated with bakanae disease of basmati rice in India

Cited by 46 publications

References 17 publications

The puzzle of bakanae disease through interactions between Fusarium fujikuroi and rice

The puzzle of bakanae disease through interactions between Fusarium fujikuroi and rice

Mapping quantitative trait loci responsible for resistance to Bakanae disease in rice

Rapid diagnosis of rice bakanae caused by Fusarium fujikuroi and F. proliferatum using loop‐mediated isothermal amplification assays

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