Mapping quantitative trait loci responsible for resistance to Bakanae disease in rice

Abstract

BackgroundBakanae or foot rot disease caused by Fusarium fujikuroi [teleomorph: Gibberella fujikuroi (Sawada) Ito] is emerging as a serious disease in rice. The disease causes both quantitative and qualitative losses to the grains under the field conditions. Breeding for resistance to Bakanae disease is a promising strategy to manage this emerging disease. In this study, we used a population of 168 F14 recombinant inbred lines (RILs) derived from two indica rice parents Pusa 1342, a highly resistant variety and Pusa Basmati 1121, a highly susceptible variety to map quantitative trait loci (QTLs) governing resistance against Bakanae disease.ResultsThe disease reaction of 168 F14 RILs were measured on the seedlings inoculated using Fusarium fujikuroi culture using high-throughput screening protocol under glasshouse conditions. Utilizing inclusive composite interval mapping, three QTLs governing resistance to Bakanae were identified, namely qBK1.1, qBK1.2 and qBK1.3 which accounted 4.76, 24.74 and 6.49 % of phenotypic variation, respectively. The major effect QTL designated qBK1.2 was mapped in 0.26 Mb region between RM5336 and RM10153. A total of 55 annotated genes were identified within the identified QTL region qBK1.2.ConclusionsThe novel QTLs identified in this study are useful resource for efficiently breeding rice cultivars resistant to Bakanae disease. This is the first report on identification of QTLs governing resistance against Bakanae in rice using inclusive composite interval mapping strategy in a RIL population.

The puzzle of bakanae disease through interactions between Fusarium fujikuroi and rice

“…Three resistant accessions have been found among the rice genotypes that contained various dwarf or semi-dwarf genes (50). Moreover, using inclusive composite interval mapping, three quantitative trait loci regulating resistance of rice basmati to F. fujikuroi were reported: qBK1.1, qBK1.2, and qBK1.3 (51).…”

Resistance mechanism of Fusarium fujikuroi to phenamacril in the field

“…Bacteria, fungi, viruses and nematodes are the major causes of rice diseases affecting production. 1 Rice bakanae disease is a worldwide disease caused by Fusarium fujikuroi Nirenberg [teleomorph Gibberella fujikuroi (Sawada) Ito in Ito & K. Kimura], which is widely distributed in all rice-growing areas, including Asia, Africa and North America. [2][3][4] On rice, F. fujikuroi can induce the following symptoms: seedling rot and elongation, grain sterility and discoloration.…”

Mapping of a major QTL, qBK1Z, for bakanae disease resistance in rice

“…Rice varieties with a single resistance gene are at an increased risk of being overcome by new pathological races (Lee et al 2017;Lee et al 2018). The development of a rice variety with a higher level of resistance against bakanae disease is a major challenge in many countries (Cumagun et al 2011;Bashyal et al 2014;Fiyaz et al 2016;Hur et al 2015;Hur et al 2016). In this study, we identi ed qBK1 z locus related to bakanae disease resistance based on genotype and phenotype analyses of homozygous recombinants on the recombinant progeny of Ilpum and Zenith, using SSR and newly developed InDel markers.…”

“…Lee et al (2019) delimited the location of qBK1 to 35 kb interval between two InDel markers, InDel 18 (23.637 Mbp) and . Fiyaz et al (2016) identi ed three QTLs (qBK1.1, qBK1.2, and qBK1.3) on chromosome 1 and one QTL (qBK3.1) on chromosome 3 from the highly resistant variety Pusa 1342. Physical location of qBK1.1 is close to qBK1 (Fiyaz et al 2016;Lee et al 2019).…”

“…Fiyaz et al (2016) identi ed three QTLs (qBK1.1, qBK1.2, and qBK1.3) on chromosome 1 and one QTL (qBK3.1) on chromosome 3 from the highly resistant variety Pusa 1342. Physical location of qBK1.1 is close to qBK1 (Fiyaz et al 2016;Lee et al 2019). Ji et al (2017) mapped a major QTL (qFfR1) in 22.56-24.10 Mbp region on chromosome 1 using 180 F 2:3 lines derived from a cross between a resistant Korean japonica variety Nampyeong and a susceptible Korean japonica line, DongjinAD.…”