Occurrence and antimicrobial resistance of pathogenic Escherichia coli and Salmonella spp. in retail raw table eggs sold for human consumption in Enugu state, Nigeria

Oj, Okorie-Kanu; Ev, Ezenduka; Co, Okorie-Kanu; Lc, Ugwu; Uj, Nnamani

doi:10.14202/vetworld.2016.1312-1319

Cited by 18 publications

(24 citation statements)

References 36 publications

(37 reference statements)

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“…Moreover, Salmonella serovars showed reduced susceptibility to streptomycin, oxytetracycline, neomycin, amoxicillin, and ampicillin. This finding is consistent with the results of Okorie-Kanu et al (2016) who reported the resistance of Salmonella spp. to penicillin G, oxacillin, and tetracyclines (100%), while was found to be highly susceptible to neomycin.…”

Section: Discussionsupporting

confidence: 93%

Prevalence of Multidrug Resistance Non-Typhoidal Salmonellae Isolated from Layer Farms and Humans in Egypt.

Diab¹,

Zaki²,

Ibrahim³

et al. 2019

jwpr

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Non-Typhoidal Salmonella (NTS) are substantial foodborne pathogens that lead to bacteremia, gastroenteritis, and focal infection. Poultry is one of the usual provenances for the development of multidrug-resistance NTS. This problem has increased in developing countries with the indiscriminate use of antibiotics in the poultry production system. The current study aimed to determine the prevalence and tendency of antimicrobial resistance of zoonotic Salmonella spp. A total of 601 samples, including cloacal samples (150) eggshell (150), egg content (15 pooled samples), layer hen carcasses (150), hand swab (68) and stool samples (68) from poultry workers, were collected from five layer chicken farms. Isolation of NTS was performed by using different cultural and biochemical methods. Moreover, Salmonella isolates were evaluated for antimicrobial susceptibility using the disc diffusion method. The cloacal samples and stool samples showed the prevalence of Salmonella spp. at approximately similar rates of 4.7% and 4.4%, respectively. Chicken isolates were identified as S. Enteritidis, S. Typhimurium, and S. Gallinarum while the human isolates were only S. Typhimurium and S. Enteritidis. The prevalence of the NTS on the surface of the eggshells (7.3%) was higher than that in the other samples. Among 12 antimicrobials tested, 86.4% resistance was found to streptomycin and oxytetracycline followed by neomycin and erythromycin (77.3%), norfloxacin and ampicillin (68.2%) across the study sites. Kanamycin and gentamicin remained sensitive by 95.5% and 90.9%, respectively. The present study indicated that layer chickens and its products are important sources for human infection with multiple-drug resistant NTS strains.

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Section: Discussionsupporting

confidence: 93%

Prevalence of Multidrug Resistance Non-Typhoidal Salmonellae Isolated from Layer Farms and Humans in Egypt.

Diab¹,

Zaki²,

Ibrahim³

et al. 2019

jwpr

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“…In current study, the AST results of Salmonella species (n = 12) isolated from milk, meat and feed samples conducted using fteen (15) selected antimicrobials coated with GN cards indicated more resistance to Cefalexin (92%), Gentamicin (92%), Cefalotin (67%), Ampicillin (50%) and Tetracycline (42%) as compare to the ndings reported in previous study [36] [37] in which more than 90-100% of salmonella spps demonstrate resistance to Tetracycline. A study conducted in Addis Ababa [38] shows that salmonella spps isolated from dairy lactating cows demonstrate resistance to Ampicillin (100%) and Tetracycline (33.33%).…”

Section: Discussionsupporting

confidence: 43%

Antimicrobial resistance status of selected bacteria isolated from animal source foods and feed in Ethiopia

Belachew¹,

Tefera²,

Tilki³

et al. 2022

Preprint

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Background Antimicrobial resistance (AMR) of major food-borne pathogens has become an increasing public health problem worldwide. In the present study, AMR profile of selected target bacterial pathogens isolated from animal source foods (ASFs) and feed was conducted. Methods A cross sectional study was conducted from August 2019 to July 2021 in high potential meat and dairy products and commercial animal feed supply chain areas of Ethiopia. A total of 642 ASFs and feed samples collected from purposively selected sampling sites were examined at microbiology laboratory of animal products, veterinary drug and feed quality assessment center. Bacterial identification and antimicrobial susceptibility test (AST) were conducted using automated Vitek 2 XL compact system. Results Out of 642 investigated samples, 24 different genera and 59 species of bacteria were identified. A total of 185 samples were positive for target bacteria of Staphylococcus aureus, Escherichia coli and Salmonella spps. The AST results showed AMR of target bacteria isolates against some of the tested antimicrobials. Of these, 83, 55 and 92% isolates of Staphylococcus aureus, Escherichia coli and Salmonella spps, respectively, showed high AMR development levels to Benzylpenicillin, Tetracycline and Cefalexin/Gentamicin. Conclusions The target bacteria isolated from ASFs and feed demonstrated multidrug resistance against some of the tested antimicrobials having public and veterinary importance. This reflects that ASFs and feed could serve as one of the sources of the spread and transmission of antimicrobial resistant bacterial pathogens. Hence, improving hygiene and sanitation management practices along the ASFs and feed supply chains, raising community awareness about the risks of AMR, strengthening good veterinary practices and further investigations on AMR are recommended.

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“…It has also been demonstrated that eggshells had significantly higher frequency of contamination by Salmonella spp. than egg contents (Okorie‐Kanu, Ezenduka, Okorie‐Kanu, Chinweokwu Ugwu, & Nnamani, ).…”

Section: Discussionmentioning

confidence: 99%

Food safety risk posed to consumers of table eggs from layer farms in Gauteng Province, South Africa: Prevalence of Salmonella species and Escherichia coli, antimicrobial residues, and antimicrobial resistant bacteria

Adesiyun

Nkuna²,

Mokgoatlheng‐Mamogobo³

et al. 2020

Journal of Food Safety

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The study conducted on 39‐layer farms in Gauteng Province, South Africa determined the prevalence and resistance of enteropathogens, and antimicrobial residues in table eggs collected from the farms. Eggs were tested for the presence of bacteria using standard methods. The resistance of bacteria to eight antimicrobial agents was determined using the disc diffusion method. Antimicrobial residues were detected in table eggs using the microbial inhibition test, enzyme‐linked immunosorbent assay (ELISA), and high performance liquid chromatography (HPLC). Risk factors for egg contamination by bacteria and antimicrobial residues were determined through a questionnaire survey of farmers. The farm prevalence of Salmonella spp. and Escherichia coli in table eggs was 7.7 and 48.7%, respectively. Nineteen (48.7%) and 2 (5.1%) of eggshells and egg contents, respectively, were positive for E. coli but only eggshells yielded Salmonella species, 2.0% (4 of 196). Overall, 71.4% of 49 E. coli isolates exhibited resistance to one or more antimicrobial agents. The farm prevalence and egg content prevalence of antimicrobial residues was 2.6 and 0.5%, respectively, with the detection of sulfonamide (79 ppb) and oxytetracycline (106 ppb). The prevalence of resistant E. coli and Salmonella spp. and the occurrence of antimicrobial residues in egg content may pose food safety and therapeutic threats to consumers.

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Occurrence and antimicrobial resistance of pathogenic Escherichia coli and Salmonella spp. in retail raw table eggs sold for human consumption in Enugu state, Nigeria

Cited by 18 publications

References 36 publications

Prevalence of Multidrug Resistance Non-Typhoidal Salmonellae Isolated from Layer Farms and Humans in Egypt.

Prevalence of Multidrug Resistance Non-Typhoidal Salmonellae Isolated from Layer Farms and Humans in Egypt.

Antimicrobial resistance status of selected bacteria isolated from animal source foods and feed in Ethiopia

Food safety risk posed to consumers of table eggs from layer farms in Gauteng Province, South Africa: Prevalence of Salmonella species and Escherichia coli, antimicrobial residues, and antimicrobial resistant bacteria

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