Antimicrobial resistance (AMR) of major food-borne pathogens has become an increasing public health problem worldwide. A cross-sectional study was conducted from August 2019 to July 2021 in high-potential meat and dairy products and commercial animal feed supply chain areas of Ethiopia. The objectives of the study was assessing AMR profile of target bacterial pathogens isolated from animal sources foods (ASFs) and feed. A total of 642 ASFs and feed samples collected from selected sampling sites were examined at the microbiology laboratory of animal products, veterinary drugs, and feed quality assessment center. Bacterial identification and antimicrobial susceptibility test (AST) were conducted using an automated Vitek 2 XL compact system. Out of 642 investigated samples, 24 different genera and 59 species of bacteria were identified. A total of 185 samples were positive for target bacteria of Staphylococcus aureus, Escherichia coli, and Salmonella Species. The AST results showed AMR of target bacteria isolates against some of the tested antimicrobials. Of these, 83%, 55%, and 92% isolates of Staphylococcus aureus, Escherichia coli, and Salmonella Species, showed high level of AMR to Benzylpenicillin, Tetracycline, and Cefalexin/Gentamicin, respectively. The target bacteria isolated from ASFs and feed demonstrated multidrug resistance against some of the tested antimicrobials having public and veterinary importance. This reflects that ASFs and feed could serve as one of the sources for the spread and transmission of antimicrobial-resistant bacterial pathogens. Hence, there is a need for improving hygiene and sanitation practices along the ASFs and feed supply chains. Besides raising community awareness about the risks of AMR, emphasis on the rational use of antimicrobials in animal health practice and further investigations on AMR are recommended.
Background Antimicrobial resistance (AMR) of major food-borne pathogens has become an increasing public health problem worldwide. In the present study, AMR profile of selected target bacterial pathogens isolated from animal source foods (ASFs) and feed was conducted. Methods A cross sectional study was conducted from August 2019 to July 2021 in high potential meat and dairy products and commercial animal feed supply chain areas of Ethiopia. A total of 642 ASFs and feed samples collected from purposively selected sampling sites were examined at microbiology laboratory of animal products, veterinary drug and feed quality assessment center. Bacterial identification and antimicrobial susceptibility test (AST) were conducted using automated Vitek 2 XL compact system. Results Out of 642 investigated samples, 24 different genera and 59 species of bacteria were identified. A total of 185 samples were positive for target bacteria of Staphylococcus aureus, Escherichia coli and Salmonella spps. The AST results showed AMR of target bacteria isolates against some of the tested antimicrobials. Of these, 83, 55 and 92% isolates of Staphylococcus aureus, Escherichia coli and Salmonella spps, respectively, showed high AMR development levels to Benzylpenicillin, Tetracycline and Cefalexin/Gentamicin. Conclusions The target bacteria isolated from ASFs and feed demonstrated multidrug resistance against some of the tested antimicrobials having public and veterinary importance. This reflects that ASFs and feed could serve as one of the sources of the spread and transmission of antimicrobial resistant bacterial pathogens. Hence, improving hygiene and sanitation management practices along the ASFs and feed supply chains, raising community awareness about the risks of AMR, strengthening good veterinary practices and further investigations on AMR are recommended.
Paracetamol (acetaminophen) is a widely used over-the-counter analgesic, antipyretic and a mild anti inflammatory drug. In several developing countries some of pharmaceutical industries sale fake, counterfeit and substandard drugs which affect the health of people. The present study investigates the comparison for the quantitative determination of various brands of paracetamol tablet using UV-Visible spectrophotometric, potentiometric and trimetric methods. Four brands (Pacimol, Paracip, Parazest, and Crocin) of paracetamol tablets having 500 mg strength were purchased from various pharmacy shops within Pari chowk and Jagat market in Greater Noida, India. Weight variation test was performed before the assay of paracetamol samples. The result of tablets weight variation (Mean ± S.D) of Crocin, Parazest, Paracip and Pacimol brand was 0.66 ± 0.014, 0.64 ± 0.010, 0.58 ± 0.007 and 0.55 ± 0.009 respectively. All brands showed different mean weight which indicates the use of different excipients in the different brands. The ranges of the amount of paracetamol content (g/tab) for paracetamol samples analyzed using UV-Visible spectrophotometric, potentiometric and trimetric methods were from 0.49195-0.52010, 0.48300-0.52100 and 0.48106-0.50110 respectively. The results indicated that all four brands of paracetamol tablets have sufficient quantity and amount is approximately similar to the company’s recommended or claimed value. Similarly, the ranges of percentage content (assay) of the analyzed samples using UV-Visible spectrophotometric, potentiometric and trimetric methods were from 98.69-104.20%, 96.60-104.20%, and 96.21-100.22% respectively. The assay results indicated that variation among all brands and this may show that different manufacturer formulates the different brands are under the IP specification. However, all of the brands of the tablets under the study were complied with the IP specification and passed for weight variation test and assay conducted on it. Hence, the drug control authority of the government should be continuously monitored the safety, quality, and efficacy of paracetamol tablet through post marketing surveillance practices, and the proper internal quality control of the pharmaceutical companies need to take further necessary steps to ensure the continuity in the establishment of the product quantity and quality. Keywords: Assay, Brands, Comparative, Determination, Paracetamol, Tablets
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