Occult hepatitis B in blood donors in Indonesia: altered antigenicity of the hepatitis B virus surface protein

Thedja, Meta Dewi; Roni, Martono; Harahap, Alida Roswita; Siregar, Nurjati Chairani; Ie, Susan Irawati; Muljono, David Handojo

doi:10.1007/s12072-010-9203-5

Cited by 27 publications

(26 citation statements)

References 34 publications

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“…In endemic countries, more than 10-15% of individuals had isolated HbcAb positivity and blood of these persons may be put down. [9] The minimum age for blood donation was 30 years in here, so we recommended that younger persons are also encouraged to donate blood. [10] Military persons have at least participated for blood donation attention to health man present in this group need also encourage for blood donation.…”

Section: Discussionmentioning

confidence: 97%

“…[15] HbcAb was positive in 20.2% of cases and its positive had significant upper HbsAb titer than HbcAb-negative persons (P < 0.000) that may be due to ancient hepatitis B infection and then cured but it should be considered that HbcAb positive may had occult hepatitis and may be transmitted to blood-recipient patients; therefore, we counsel not to use their blood, especially for immunodeficiency patients such as transplant. [9] Therefore, for better safety of blood, we consider anti-HBc as shown to be a better screening parameter than HBsAg [16,17] The prevalence of HbcAb positivity in our country is very high than European countries that may be owing to intermediate prevalence of hepatitis B infection and there is high risk of transmission of HBV through blood donation ,and it caused deferral blood increase, in the way we considered HbcAb should be screened before blood donation. [8,18] In here, at least 3 times blood donation was seen in 10.4% and 11 time in 0.2%.…”

Section: Discussionmentioning

confidence: 99%

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Hepatitis B core antibody in blood donor in sistan-balutuestan province of Iran

Moghaddam¹,

Khosravi²,

Ghorbani

et al. 2010

Indian J Med Sci

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Hepatitis B core antibody in blood donor in sistan-balutuestan province of Iran

Moghaddam¹,

Khosravi²,

Ghorbani

et al. 2010

Indian J Med Sci

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“…Nested polymerase chain reaction (PCR) using two sets of primers (S2-1/S1-2 [5′-CAA GGT ATG TTG CCC GTT TG-3′/5′-CGA ACC ACT GAA CAA ATG GC-3′] and S088/S2-2 [5′-TGT TGC CCG TTT GTC CTC TA-3′/5′-GGC ACT AGT AAA CTG AGC CA-3′]) was performed targeting an S gene segment encoding the "a" determinant of HBsAg. [30][31][32][33][34] Denaturation, annealing, and extension were done at 94°C for 30 seconds, 55°C for 30 seconds, and 72°C for 90 seconds/1 minute for the first/second PCR rounds (35/ 30 cycles), respectively. This nested PCR method was capable of detecting HBV DNA at very low titer (< 6 IU/mL), as validated using a panel of sera with known HBV DNA titers based on quantitative real-time PCR (Cobas TaqMan™ HBV Test; Roche Diagnostics, Indianapolis, IN) (Supplemental Table 1).…”

Section: Methodsmentioning

confidence: 99%

High Prevalence of Hepatitis B Virus Infection in Young Adults in Ternate, Eastern Indonesia

Ie¹,

Turyadi²,

Sidarta³

et al. 2015

The American Journal of Tropical Medicine and Hygiene

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Abstract. The incidence of hepatitis B virus (HBV) infection has been declining thanks to the universal hepatitis B infant immunization program. Nevertheless, young adults born before the program implementation might have acquired HBV in early childhood or remain susceptible to infection. This study aimed to evaluate hepatitis B epidemiology in asymptomatic young adult population in Ternate, eastern Indonesia. Serum samples of 376 subjects (aged 17-25, mean 19.82 ± 1.69 years; male/female 138/238) were screened for HBV parameters serologically (HBV surface antigen [HBsAg]; its antibody [anti-HBs]; anti-core antigen [anti-HBc]), and molecularly (HBV DNA). HBsAg, anti-HBc, anti-HBs, and HBV DNA prevalence were 15.7%, 36.2%, 24.2%, and 27.9%, respectively, with male predominance. Of all subjects, 13.0% were HBsAg negative with detectable HBV DNA (occult hepatitis B [OHB]), and 56.4% showed negativity for all seromarkers. This population showed high hepatitis B prevalence with substantial occurrence of OHB. However, a high percentage of the population were still susceptible and at risk of HBV infection. This study emphasizes the necessity to improve prevention strategies to screen and manage HBV carriers, including the adoption of catch-up or booster vaccination targeted to young adult populations. Investigations on the roles of host-virus interactions associated with OHB and its implications are warranted.

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“…In the analysis of healthy stem cell donors from South Korea, OHB was detected in 15.3%, which, like in the case of a significant occurrence of OHB in the population, can be explained by the high prevalence of hepatotropic viruses in this geographic region [20]. In Indonesia, occult HBV was detected in 8.1% of blood donors [44]. In Iran, since the routine neonatal vaccination against HBV was adoption in 1993, the prevalence of the virus was about 1.7% [37].…”

Section: Resultsmentioning

confidence: 95%

RESULTS OF GENOTYPING HEPATITIS VIRUS B IN HBsAg-NEGATIVE BLOOD DONORS IN ASTANA, KAZAKHSTAN

Ostankova

Семенов

Буркитбаев

et al. 2018

Russian Journal of Infection and Immunity

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Abstract. The prevalence of HBV infection is estimated by the frequency of occurrence of HBsAg and varies depending on the geographic region. Chronic infection is characterized by a stable presence of HBsAg for 6 months, with the exception of the occult form of the disease, characterized by the absence of HBsAg, an extremely low level of HBV DNA in the blood serum. The problem of identifying occult HBV (ocHBV) is especially relevant because of the development of transplantology and transfusiology. However, serological screening of donor blood used in the Russian Federation and Central Asian countries does not reveal HBV seronegative donors. Since HBV infection is possible with the introduction of small doses of the virus, the importance of using complex molecular methods for detecting donor ocHBV is obvious, despite the low viral load, since donor blood is used predominantly in patients with severe course of various diseases characterized by increased susceptibility to HBV because of immunosuppression. The aim of our work was to study the characteristics of the genetic structure of the ocHBV in donors in Astana, Kazakhstan. A total of 500 blood plasma samples from HBsAg-negative donors were obtained in 2012 from residents of Kazakhstan, Astana. Using the method, we proposed to detect HBV DNA with a low viral load, HBV was detected in 9.4% of donors. Serological markers were found in 12.7% of patients with HBV DNA, 8.5% had HBcor IgG antibodies, 4.2% had HBcor IgG and HBe IgG antibodies at the same time. Thus, in 41 (87.3%) of the blood donor, ocHBV was seronegative. Based on the phylogenetic analysis of the 47 isolates showed that the HBV of genotype D (95.75%) prevails in the examined group in comparison with HBV of genotype A (4.25%). HBV subgenotypes are represented in the following ratios: D1 -46.8%, D2 -17.05%, D3 -31.9%, A2 -4.25%. In a comparative analysis, the distribution of HBV subgenotypes in the group with ocHBV and in the case of the manifest form in donors in the Republic of Kazakhstan significantly differed -χ 2 = 14.027 at p = 0.0072, d f = 4. The incidence of HBV D3 with ocHBV (31.9%) exceeded that of patients with a manifest form (7.4%). The relative risk of occult form of disease in patients with the subgenotype D3 is significantly higher (RR = 1.572, CI: 1.179-2.096, p = 0.0208). When assessing the picture of HBV diversity on the material of the group including HBsAg-negative and HBsAg-positive blood donors, it is evident that the genetic relationship of the manifestations of the manifest HBV and the ocHBV of genotype D is obvious. Among the isolates are both similar in nucleotide sequences with those previously described in various regions of Europe and Central Asia, and circulating in the territory of the Republic of Kazakhstan, which indicates an independent homologous evolution of HBV in the region. The high incidence of ocHBV among HBsAg-negative blood donors is indicative not only of the widespread prevalence of the occult form of the disease course in the population and the inadequacy ...

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Occult hepatitis B in blood donors in Indonesia: altered antigenicity of the hepatitis B virus surface protein

Cited by 27 publications

References 34 publications

Hepatitis B core antibody in blood donor in sistan-balutuestan province of Iran

Hepatitis B core antibody in blood donor in sistan-balutuestan province of Iran

High Prevalence of Hepatitis B Virus Infection in Young Adults in Ternate, Eastern Indonesia

RESULTS OF GENOTYPING HEPATITIS VIRUS B IN HBsAg-NEGATIVE BLOOD DONORS IN ASTANA, KAZAKHSTAN

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