Number of spermatogenic cells and testes histology of mice (Mus musculus L.) after treated with different times and intervals of red calliandra (Calliandra calothyrsus Meissn.) leaf extract

Abstract

Tannin compounds in the form of tanic acid are known to inhibit the acrosine activity of spermatozoa and plasminogen activator, which is an antifertility mechanism. Red Calliandra (Calliandra calothyrsus Meissn.) leaves have the potential to be developed as herbal medicines, but these leaves contain high condensed tannins, so research needs to be done to determine the effect of red kaliandra leaf extract on the reproduction of male mice.This research used a completely randomized design (CRD) in a factorial patern with four doses and three replications, i.e. control (NaCl 0,9%), doses of red caliandra leaf extract of 2, 4 and 6 mg/kg body weight and time interval of 7, 14 and 21 days. The testes organ were taken and processed histologically by Paraffin Methods and Hematoxylin-Eosin staining. Parameters observed were the amount of spermatogonia, spermatocyte and spermatid cells of male mice. A directly calculation method was done by observed the cells of tubulus seminiferous histology for each treatment used a microscope camera and Image Raster software (Optilab). The data were analyzed with One Way Anova and Duncan Multiple Range Test (DMRT). Calliandra calothyrsus leaf extract with different doses and time intervals decreased the amount of spermatogonia, spermatocytes and spermatids cells in the testes tubulus seminiferous of male mice, the arrangement of spermatogenic cells are increasingly irregular and the lumen were not full filled with spermatozoa cells.

Influence of Mangrove Leaf Extract Rhizophora Apiculata Blume on The Number of Spermatogonium Cells in White Rats (Rattus Norvegicus L.)

“…In this case, the Flavonoid compounds in the extract stimulate estrogen synthesis in mammals because their chemical structure is similar to that of estrogen. So that it can cause a decrease in FSH and LH production, and a decrease in LH synthesis will trigger a decrease in hormone synthesis by the Leydig cells in the testes [5].…”

“…Harris and Septiana [4], in their research on the effect of giving brotowali plant extract on the reproductive system of male mice, stated that giving brotowali extract had an apparent effect in the form of a decrease in the number of spermatogenic cells in each section of the seminiferous tubules of mice. Pranadya et al, [5], in their research on the number of spermatogenic cells and testicular histology after administration of red calliandra leaf extract, stated that the given calliandra leaf extract caused a decrease in the number of spermatogenic cells in the seminiferous tubules of white rats (Rattus norvegicus L.) Yasmin et al, in their research on the effect of giving bitter gourd ethanol extract to seminiferous tubule tissue in male mice, stated that giving bitter gourd ethanol extract could reduce the thickness of the seminiferous tubules with the optimal dose of 1,120 mg/kgBB day. While Anzila et al, [6] concluded that the administration of basil extract on mouse males (Rattus norvegicus L.) Balb-c strain with 200 mg/kg BW has the potential to enhance the process of spermatogenesis or as a fertility agent.…”

The Effect of Kepel Fruit Extract on The Number of Spermatogenic Cells in Mice (Mus musculus)

“…Apoptosis sel spermatogenik berhubungan dengan dampak sitotoksik. Sedangkan efek sitostatik melibatkan penghambatan sel spermatogenik yang secara aktif berkembang biak serta pengurangan metabolisme sel spermatogenik dengan mengganggu keseimbangan sistem hormonal (Pranadya et al, 2019).…”