Influence of Mangrove Leaf Extract Rhizophora Apiculata Blume on The Number of Spermatogonium Cells in White Rats (Rattus Norvegicus L.)

Abstract

Many cases of infertility are caused by several things, one of which is a disruption of the hormone cycle, which results in a decrease in the levels of hormones produced. Objective: To determine the effect of Rhizophora apiculata Blume mangrove leaf extract on the number of spermatogonium cells of white rats (Rattus et al.). Method: Using the Tru-experiment with a completely randomized design (CRD), followed by a simple random sampling technique and analyzed using the ANOVA test and the Tukey-HSD follow-up test (ɑ=0.05) with IBM SPSS version 26. The study population consisted of 50 rats. White male, then randomized into 24 tails with criteria of 8-10 weeks old, healthy body, and average weight of 150-200 grams. Results: The higher the concentration of the extract given, the less the number of spermatogonium cells produced, with the average number of spermatogonium cells obtained being P0: (71 cells), P1: (47 cells), P2: (30 cells), and P3: (19 cells). Conclusion: Mangrove leaf extract has a significant negative effect on decreasing the number of Spermatogonium cells in white rats (р˂0.05). This is due to the content of active metabolite compounds such as tannins, alkaloids, saponins, and flavonoids, which work as antifertility through hormonal effects, which can reduce the number of cells in spermatogonia..