Walleye dermal sarcoma was transmitted under experimental conditions to yellow perch Perca flavescens. Fish (20 weeks posthatch) were challenged with cell-free tumor filtrates by topical application on the right flank and then held for observation for 25 weeks in 15ЊC dechlorinated municipal water. Additional treatment groups included yellow perch challenged with cell-free filtrates of walleye discrete epidermal hyperplasia, yellow perch discrete epidermal hyperplasia, normal yellow perch skin (control), and normal walleye skin (control). Walleye dermal sarcoma was first observed on yellow perch at 20 weeks postexposure, at which time the tumors were small (1-2-mm), slightly raised masses on the right flank of the fish. At the end of the 25week study, walleye dermal sarcoma was grossly observed in 42% (22 of 53) of the remaining fish and was confirmed by microscopic examination in an additional 29% (9 of 31). Gross and microscopic evaluation of fish at 25 weeks postexposure did not reveal development of lesions in any of the other treatment groups. This study extends the host range of experimentally transmitted walleye dermal sarcoma virus to include the yellow perch.