Investigation of epizootic papillomatosis in bluegill Lepomis macrochirus (Rafinesque 1810) using next‐generation sequencing

Metagenomic Next-Generation Sequencing Reveals Miamiensis Avidus (Ciliophora: Scuticociliatida) in the 2017 Epizootic of Leopard Sharks (Triakis Semifasciata) in San Francisco Bay, California, Usa

“…In this study, we describe an epizootic of wild leopard sharks characterized by stranding behavior and meningoencephalitis, and provide strong molecular and histological evidence that implicates the ciliated protozoan, M. avidus, as the candidate pathogen associated with the SF Bay mass mortality event. We identified M. avidus through an unbiased, NGS-based approach, which we and others have previously used in a wide range of human and non-human infectious disease investigations (Dervas et al 2017;Dill et al 2017;Langelier et al 2017;Pfaff et al 2017;Wilson et al 2017;Doan et al 2016;Zylberberg et al 2016;Wilson et al 2015;Masuda et al 2014;Stenglein et al 2014;Wilson et al 2014;Stenglein et al 2012).…”

“…However, PCR tests specific candidates and may be limited by available genetic information. Metagenomic next-generation sequencing (mNGS) provides an unbiased alternative, which has been used successfully in human and animal infections (Dervas et al 2017;Dill et al 2017;Langelier et al 2017;Pfaff et al 2017;Wilson et al 2017;Doan et al 2016;Zylberberg et al 2016;Wilson et al 2015;Masuda et al 2014;Stenglein et al 2014;Wilson et al 2014;Stenglein et al 2012;Kistler et al 2008). Through the analysis of all nucleic acids in a sample, mNGS can simultaneously test for all known organisms, and can also identify novel pathogens including distantly related species.…”

Metagenomic next-generation sequencing reveals Miamiensis avidus (Ciliophora: Scuticociliatida) in the 2017 epizootic of leopard sharks (Triakis semifasciata) in San Francisco Bay, California

“…In this study, we describe an epizootic of wild leopard sharks characterized by stranding behavior and meningoencephalitis, and provide strong molecular and histological evidence that implicates the ciliated protozoan, M. avidus, as the candidate pathogen associated with the SF Bay mass mortality event. We identified M. avidus through an unbiased, NGS-based approach, which we and others have previously used in a wide range of human and non-human infectious disease investigations (Dervas et al 2017;Dill et al 2017;Langelier et al 2017;Pfaff et al 2017;Wilson et al 2017;Doan et al 2016;Zylberberg et al 2016;Wilson et al 2015;Masuda et al 2014;Stenglein et al 2014;Wilson et al 2014;Stenglein et al 2012).…”

“…However, PCR tests specific candidates and may be limited by available genetic information. Metagenomic next-generation sequencing (mNGS) provides an unbiased alternative, which has been used successfully in human and animal infections (Dervas et al 2017;Dill et al 2017;Langelier et al 2017;Pfaff et al 2017;Wilson et al 2017;Doan et al 2016;Zylberberg et al 2016;Wilson et al 2015;Masuda et al 2014;Stenglein et al 2014;Wilson et al 2014;Stenglein et al 2012;Kistler et al 2008). Through the analysis of all nucleic acids in a sample, mNGS can simultaneously test for all known organisms, and can also identify novel pathogens including distantly related species.…”