Nucleosomes and subnucleosomes: heterogeneity and composition

Bakayev, Valery V.; Bakayeva, T. G.; Varshavsky, Alexander

doi:10.1016/0092-8674(77)90079-4

Cited by 109 publications

(57 citation statements)

References 18 publications

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“…Notielheless, it is reasonable to conclude that the DNA quantified by this technique does in fad have an ON-like structure since essentially all the DNA seeti on PAGE can be interpreted as such, including the non-oligomeric 50-110 bp species (24)(25)(26)(27). That this 50-110 bpsize speeies cannot itself account fora predominant portion ofthe total DNA tneasured at 7' (, is suggested by the absence of any correlation al T,, between the intensity of its autoradiographic signal and the total amount of DNA measured by CIE. (It is recognized that this intensity may not be a precise reflection of DNA amount beeauseof possible variation in labelling elficiency among the DNA species.…”

Section: Discussionmentioning

confidence: 99%

Haemodialysis as a model for studying endogenous plasma DNA: oligonucleosome-like structure and clearance

Rumore

Muralidhar

Lin

et al. 1992

Clinical and Experimental Immunology

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SUMMARYThe rale of clearance of exiracellular plasma DNA in man ha.s importani inipiitalions for palhogenelie mechanisms in systemic lupus erythemalosus (SLE), as well as for eeriain oihcr clinical states. Present knowledge of this parameter is derived exelusively from studies of injected, naked DNA in animals. Recent informalion indicates that the physiologic form of plasma DNA in SLE is that of oligonucleosome-like molecules rather than of naked DNA and eonsists of multimcric complexes of DNA bound to histone, probably arising from an apoplotic process. In order to study the rale at which ihese oligonucleosome-like complexes are removed from plasma and to do so in man rather than experimental animals, we exploited the observation that during haemodialysis large amounts of DNA are released, apparently within the dialysis coil, inlo the paiieni's plasma. Since this release appears lo cease promptly with termination of the procedure, it offered the potential for estimating the rate of removal of such DNA from human plasma. Moreover, if that DNA. as postulated, were shown to possess an oligonucleosome-like structure resembling that fotind endogenously in human SLE, the relevance of such information to the human disease state would be further enhanced. The present results support the conclusion that DNA released into plasma during haemodialysis possesses such an oligonucleosome-like structure. The plasma half-life of ihat DNA in man was found nol lo exceed 4 min. The highly dynamic state thus implied for extracellular endogenous plasma DNA in man has important implications for pathogenetic mechanisms dependent on dsDNA in SLE. Moreover, individuals undergoing chronic hacmodialysi,s, who are thereby exposed to a very large cumulative amount of such DNA., might serve as models for studying its long-term sequelae.

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Section: Discussionmentioning

confidence: 99%

Haemodialysis as a model for studying endogenous plasma DNA: oligonucleosome-like structure and clearance

Rumore

Muralidhar

Lin

et al. 1992

Clinical and Experimental Immunology

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“…In the model we used the left-handed superhelical coil of 140-145 bp DNA proposed [ 191 as a basis for a linear histone array [20]. The DNA coil was extended by -10 bp at each end [ 131 to obtain 16.5 bp Hl -containing MN2 [6]. This additional segments can interact with Hl (H5) histone or with non-hi&one proteins, especially HMG type [6,7,21].…”

Section: Discussionmentioning

confidence: 99%

“…The DNA coil was extended by -10 bp at each end [ 131 to obtain 16.5 bp Hl -containing MN2 [6]. This additional segments can interact with Hl (H5) histone or with non-hi&one proteins, especially HMG type [6,7,21]. The model being not so detailed as that in [ 151 reflects only the major sites of histone contacts with DNA.…”

Section: Discussionmentioning

confidence: 99%

“…We have separated 4-5 main structural types of mononucleosomes: along with core particles (MNl), mononucleosomes were identified containing an additional segment of linker DNA and either Hl histone or non-histone protein HMG 14 or 17 [6-g]. We also discovered a set of small subnucleosomal particles which contained short DNA fragments and histones or non-histone proteins [3,6,7]. Two of them (SN2 and 3) comprising short DNA fragment and HMG protein originated apparently from transcriptionally active chromatin [8].…”

Section: Introductionmentioning

confidence: 99%

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Histone‐containing subnucleosomes: possible implications to nucleosome structure

Bakayev¹,

Domansky²,

Bakayeva³

1981

FEBS Letters

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“…Identification of variant nucleosome subtypes is based on correlation of second-dimension DNA and protein patterns [9,19,20] and strengthened in the case of Dl-mononucleosomes by hybridization mapping with heterochromatic, AT-rich DNA sequences 1211 and by nucleosome solubility in 0.1 M NaCl [lo].…”

Section: Hybridizationmentioning

confidence: 99%

A unique nucleoprotein structure associated with the Drosophila melanogaster 18–28 S rDNA nontranscribed spacer

Levinger

Nass

1986

FEBS Letters

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We have detected unique nucleoprotein particles specific for the 18-28 S rDNA nontranscribed spacer of Drosophila melanogaster. The particles migrate between di-and trinucleosomes on nucleoprotein gels, and are between mono-and dinucleosomal in DNA length. These migration properties suggest that the nontranscribed spacer particles could have a protein component larger than a histone core. The variant nucleoprotein structures map primarily within the nontranscribed spacer 235 base pair internal subrepeat, which is AT-rich and possesses a 50 base pair sequence homologous to the RNA polymerase I binding site. Nucleoprotein particle 18-28 S rDNA(D. melanogaster)

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Nucleosomes and subnucleosomes: heterogeneity and composition

Cited by 109 publications

References 18 publications

Haemodialysis as a model for studying endogenous plasma DNA: oligonucleosome-like structure and clearance

Haemodialysis as a model for studying endogenous plasma DNA: oligonucleosome-like structure and clearance

Histone‐containing subnucleosomes: possible implications to nucleosome structure

A unique nucleoprotein structure associated with the Drosophila melanogaster 18–28 S rDNA nontranscribed spacer

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