Nuclear DNA content of three Eucalyptus species estimated by flow and image cytometry

Abstract

Previous flow cytometry (FCM) analyses delivered nearly equal mean values of nuclear 2C DNA content for Eucalyptus grandis Hill ex Maiden and E. urophylla S. T. Blake (1.33 pg and 1.34 pg, respectively), whereas E. globulus Labill. presented distinct mean values (1.09, 1.13 and 1.40). These differences have been attributed to the different methodological approach, utilised plant cultivar and presence of intrinsic metabolic compounds that affect fluorochrome fluorescence. In the present study, a FCM and image cytometry (ICM) design, following international consensus criteria, were adopted to reassess the nuclear DNA contents of the above-mentioned Eucalyptus species. Statistical analyses revealed either similar or discrepant nuclear DNA contents, depending on the standard species used and whether FCM or ICM was employed. Our results demonstrated that 2C DNA values obtained by FCM and ICM were most uniform when Solanum lycopersicum was used as a standard. Moreover, the values obtained for E. grandis and E. urophylla were close, but differed as much as 24.63% in relation to previous data, and E. globulus proportionally varied up to 25%. New DNA content values are suggested for these eucalypt species.

High‐resolution genetic maps of Eucalyptus improve Eucalyptus grandis genome assembly

“…This is consistent with the small phylogenic distance between these two interfertile species of the Symphyomyrtus subgenus (Potts & Dungey, ; Steane et al ., ). Moreover, their genomes were found to be close in terms of physical size (Grattapaglia & Bradshaw, ; Praça et al ., ) and in terms of genome content, as shown by the robust alignment of E. urophylla sequences on the BRASUZ1 genome. This closeness of genome structure was also reported between E. grandis and E. globulus , two species belonging to different sections: Latoangulatae and Maidenaria .…”

ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

“…According to Praça et al (2009), the three species used for hybrids investigated here have 1.34 pg (E. urophylla), 1.33 pg (E. grandis) and 1.09 to 1.40 pg (E. globulus) DNA content. The DNA content (2C) species and hybrids of commercially important Eucalyptus ranges from 0.77 to 1.47 pg (Grattapaglia and Bradshaw, 1994).…”

Cariótipo E Conteúdo De Dna Nuclear De Quatro Espécies Do Gênero Costus L.: Uma Contribuição Citotaxonômica E Evolutiva/Karyotype and Nuclear Dna Content of Four Species of the Genus Costus L.: A Cytotaxonomic and Evolutionary Contribution

“…Segundo Feitoza et al (2017), o genoma destas plantas é pequeno e diante dos valores obtidos no presente trabalho, a diferença que as distingue parece ser baixa (2,97 pg, 2,94 pg, 2,84 pg e 2,76 pg) (Tabela 5) e (figura 2). Praça, et al (2009) e Praça-Fontes et al (2011), pois, quando analisado com os demais, mostrou-se como um padrão primário de referência ideal para mensuração por meio do citômetro de fluxo.…”

“…Neste trabalho foi realizado o processamento combinado das espécies alvo e padrão e a centrifugação (diluição da suspensão nuclear ou remoção do citosol), porém, esses procedimentos minimizam, mas não impedem a interferência de metaboólitos secundários (Greilhuber, 2005;Price et al, 2000;Praça et al, 2009). O processamento reduz a variabilidade e melhora a padronização, no entanto, em caso de metabólitos secundários, como resultado, a amostra e núcleos padrão são influenciados na mesma medida (Dolezë et al, 1991;Baranyi e Greilhuber, 1999;Bennett et al, 2008).…”