ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

Oliveira, Leandro Silva de; Xavier, Aloísio; Otoni, Wagner Campos; Campos, José Marcello Salabert de; Viccini, Lyderson Facio; Takahashi, Elizabete Keiko

doi:10.1590/1806-90882017000100014

Cited by 5 publications

(1 citation statement)

References 52 publications

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“…europaea var. sylvestris [39], Eucalyptus globulus [45], Neolamarkia cadamba [46], and Disanthus cercidifolius [47]. In this study, the histogram obtained from the leaf nuclei of the in vitro plants and the donor plants revealed that the fluorescence peaks of all of the samples were consistent, indicating that the ploidy level of the regenerated plants was maintained, thereby ensuring the efficacy this protocol plant without compromising its genetic integrity.…”

Section: Discussionsupporting

confidence: 51%

Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of Maerua crassifolia Forssk

Alatar

Qahtan

Abdel-Salam

et al. 2023

Forests

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The difficult propagation of shrub and tree species and their extensive exposure to grazing threaten their abundance and lead to the necessity to find alternative means of propagation for these species. In vitro micropropagation techniques, viz., tissue culture, offer a promising tool for the rapid, cost-effective, and efficient propagation of different plant species. In the current study, a rapid and efficient in vitro multiplication protocol was developed for the micropropagation of Maerua crassifolia Forssk. Our results revealed that Murashige and Skoog (MS) medium with 7.5 µM of 6-benzylaminopurine (BA) and 1.0 µM of 1-naphthaleneacetic acid (NAA) led to the highest shoot formation (13.9 shoots per explant in 85.7% of the cultivated hypocotyls) among all other treatments. The best in vitro root formation was obtained on half-strength MS medium with 1.0 µM of indole 3-butyric acid (IBA) as 94.1% of the cultivated shoots formed 6.8 roots per microshoot on average. Ninety percent of the rooted plantlets were successfully acclimatized and are currently growing in the botanical garden of the Botany and Microbiology Department, King Saud University, Riyadh, Saudi Arabia. The genetic fidelity of the micropropagated plants was authenticated via flow cytometry. The results of the current study explained a simple, cost-effective, and efficient protocol for the micropropagation of the endangered M. crassifolia trees.

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Section: Discussionsupporting

confidence: 51%

Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of Maerua crassifolia Forssk

Alatar

Qahtan

Abdel-Salam

et al. 2023

Forests

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Effect of light intensity on in vitro introduction and multiplication of Eucalyptus grandis × Eucalyptus urophylla

Souza

Fernandes

Silva

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Molecular Techniques for the Detection of Ploidy Level and Genetic Fidelity of Regenerated Plantlets

Rohela,

Saini

2023

Genome Size and Genetic Homogeneity of Regenerated Plants: Methods and Applications

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Most of the medicinal, aromatic and other commercially important crops are poor rooters, and some of them are sessile in terms of seed production; hence these plants are very difficult to propagate either through stem cuttings based vegetative propagation or through seedlings based sexual propagation. During the last two decades, plant tissue culture has emerged as an alternative technique for the propagation of plants with commercial importance. Majorly, the somatic tissues, viz., leaf, node and shoot tip, are being used as explants for the production of genetically similar plantlets through tissue culture studies. Recently, abnormalities with respect to ploidy level and genetic fidelity have been reported in In Vitro regenerated plantlets. This is mainly due to the usage of synthetic chemicals or artificial plant growth regulators in In Vitro culture studies, the fragile nature of callus and exposure of cultures to artificial light sources. In order to ensure the commercial production of genetically true clones of commercial plants, nowadays it has become an obligatory step to assess the ploidy level and genetic fidelity of regenerated plantlets with that of mother plants. This book chapter focussed on different molecular techniques which are in use for the detection of ploidy level and genetic fidelity of In Vitro micro propagated plantlets.

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ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

Cited by 5 publications

References 52 publications

Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of Maerua crassifolia Forssk

Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of Maerua crassifolia Forssk

Effect of light intensity on in vitro introduction and multiplication of Eucalyptus grandis × Eucalyptus urophylla

Molecular Techniques for the Detection of Ploidy Level and Genetic Fidelity of Regenerated Plantlets

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