Nova tripanozomiaze humana: estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n. sp., ajente etiolojico de nova entidade morbida do homem

Chagas, Antonio Carlos Palandri

doi:10.1590/s0074-02761909000200008

Cited by 1,317 publications

(703 citation statements)

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“…A wide variation was also observed in other dimensions, such as the distance between the posterior end and the kinetoplast, and between the posterior end and the nucleus. Similar observations were made by Chagas (1909) and Hoare (1972) when they described the trypomastigote forms of T. cruzi, which also presented morphometric variation in total length [16.3-21.8 µm according to Hoare (1972)]. Pleomorphism in T. rangeli, Trypanosoma minasense and Trypanosoma saimirii was also observed by Ziccardi and Lourenço-de-Oliveira (1998) in primates of the Brazilian Amazon.…”

Section: Discussionsupporting

confidence: 77%

Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil

et al. 2018

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Parasites of the genusTrypanosomaare unicellular flagellated microorganisms of theTrypanosomatidae. This study describes an isolate of the genusTrypanosomanaturally infectingRhipicephalus microplusticks, characterized through molecular, morphological and biological analysis. Trypanosome cultures, designated strain P1RJ, were obtained by isolation fromR. microplushaemolymph in cultures of the tick cell line IDE8. After isolation, strain P1RJ grew well axenically in L15B medium at temperatures of 30, 32 and 34 °C. The new trypanosome remained stable in axenic culture over 14 passages in L15B at 30 °C and was successfully cryopreserved and resuscitated. Morphometric analysis was performed on randomly selected developmental forms. 18S rRNA and 24Sα rDNA sequence analyses confirmed that strain P1RJ is a new species of the genusTrypanosoma. The nucleotide sequences described were submitted to Genbank. Pathogenicity, involvement in vertebrate hosts, epidemiology, developmental cycle and transmission mechanisms of strain P1RJ are still unknown. Therefore, more studies will be necessary to determine life cycle aspects of this trypanosome, for which we propose the nameTrypanosoma rhipicephalissp. nov.

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“…A tuberculose era uma doença conhecida na Europa desde o início da Idade Média, tendo sido a primeira doença infecciosa a ser reconhecida como tal, ou seja, transmissível de pessoa para pessoa, pelo médico, matemático, geógrafo, astrônomo e poeta Girolamo Fracastoro (1478-1553) em 1546, uma idéia revolucionária na época 24 10 , simultaneamente nos idiomas português e alemão (esse último, juntamente com o inglês e francês, era um dos idiomas de divulgação científica da época). Carlos Chagas já havia publicado em 1909, na mesma revista, os achados sobre a morfologia e o ciclo evolutivo do Trypanosoma cruzi, o agente etiológico da nova doença 7 . Nos anos 1909-1913, ele publicou algumas de suas observações em revistas francesas e alemãs 5 6 8 9 11 .…”

Section: Aspectos Históricos Do Processo De Indicação Ao Prêmio Nobelunclassified

O processo de avaliação em ciência e a indicação de Carlos Chagas ao prêmio Nobel de Fisiologia ou Medicina

Pittella

2009

Rev. Soc. Bras. Med. Trop.

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Revista da Sociedade Brasileira de Medicina Tropical 42(1):67-72, jan-fev, 2009 ARTIGO DE OPINIÃO/OPINION ARTICLEAo completar 100 anos da descoberta da doença de Chagas, permanecem ainda incertezas e especulações a respeito da história da indicação de Carlos Chagas (1879-1934 RESUMOUma das maiores realizações na história da medicina foi a descrição da doença de Chagas pelo médico e cientista Carlos Chagas. Ao completar 100 anos da descoberta da doença de Chagas, permanecem ainda especulações a respeito das duas indicações oficiais de Carlos Chagas à maior premiação mundial em ciência, o Nobel, em 1913 e 1921. Admite-se que a não premiação do genial cientista possa ter ocorrido em razão da forte oposição que enfrentou no Brasil por parte de alguns médicos e pesquisadores da época, que chegaram mesmo a questionar a existência da doença de Chagas, influenciando a decisão do Comitê Nobel para não premiá-lo. A análise do banco de dados dos arquivos do Prêmio Nobel, com a revelação dos nomes de indicadores, indicados e ganhadores do prêmio, cobrindo o período 1901-1951, trouxe informações não apenas sobre o que era considerada realização científica na época, mas também sobre quem eram os cientistas importantes e quais eram as relações entre eles. O não reconhecimento das descobertas de Carlos Chagas pelo Comitê Nobel parece ser mais corretamente explicado por esses fatores do que pelo impacto negativo da oposição local.Palavras-chaves: Carlos Chagas. Doença de Chagas. Prêmio Nobel. Medicina. História.Medicina. História. ABSTRACTOne of the greatest achievements in the history of medicine was the description of Chagas disease by the physician and scientist Carlos Chagas. A hundred years after the discovery of the disease, speculation still remains regarding the two official nominations of Carlos Chagas for the Nobel Prize, the biggest worldwide scientific award, in 1913 and in 1921. It has been accepted that the reason why the prize was not awarded to this brilliant scientist may have been the strong opposition that he faced in Brazil, from some physicians and researchers of that time. They went as far as questioning the existence of Chagas disease, thereby possibly influencing the decision of the Nobel Committee not to award the prize to him. Analysis of the database of the Nobel prize archives, with the revelation of the names of nominators, nominees and prizewinners spanning the years 1901-1951, brought information not only about what was considered to be a scientific achievement at that time, but also about who the important scientists were and what the relationships between them were. The non-recognition of Carlos Chagas' discoveries by the Nobel Committee appears to be more correctly explained by these factors than by the negative impact of the local opposition.

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“…Discovered in the early 20 th century by the celebrated brazilian scientist Carlos Chagas, the parasitic disease bearing his name is caused by the protozoa Trypanosoma cruzi (Chagas 1909). Epidemiological data show that around nine million people are infected by T. cruzi in the world, with most cases in Latin America.…”

mentioning

confidence: 99%

Expanding the knowledge of the chemical structure of glycoconjugates from Trypanosoma cruzi TcI genotype. Contribution to taxonomic studies

Fonseca

Garcez

Penha

et al. 2016

An. Acad. Bras. Ciênc.

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One of the main obstacles to the treatment of Chagas disease is the genetic and phenotypical variance displayed by T. cruzi strains, resulting in differences in morphology, virulence, pathogenicity and drug susceptibility. To better understand the role of glycoconjungates in Chagas disease, we performed the molecular characterization of the O-linked chains from mucins and glycoinositolphospholipids (GIPLs) of the Silvio X10 clone 1 strain. We demonstrated the presence of a β-galactofuranose (β-Galf) unity linked to the O-4 position of the α-N-acetylglucosamine (α-GlcNAc)O-4 in Tc-mucins. GIPLs analysis showed that the lipidic portion is exclusively composed of ceramide and the PI-oligossacharidic portion contains the Man4(AEP)GlcN-Ins-PO 4 core, substituted by ethanolamine-phosphate (EtNP) on the third distal mannose from inositol, which may or may not have a terminal β Galf unity. These results confirm the classification of the Silvio X10/1 strain in group T. cruzi I. Again, it is noted that the study of T. cruzi surface glycoconjugates confirm the molecular results and the hypothesis that surface glycoconjugates may be interesting biomarker for the differentiation of trypanosomatid strains.

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“…Current drugs for the treatment of Chagas' disease Chagas' disease remained without an effective treatment for several decades after its original description in 1909 [6]. Nifurtimox and benznidazole, discovered over 40 years ago and still the only available drugs for the specific treatment of Chagas' disease, are limited by toxicity and low efficacy in the established chronic form of the disease [7].…”

mentioning

confidence: 99%

Match-making for posaconazole through systems thinking

Fügi

Kaiser

Tanner

et al. 2015

Trends in Parasitology

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“…Aunque la enfermedad de Chagas se reporta desde hace casi 100 años (2), poco se conoce de su patogénesis. El curso clínico es muy variable; se presenta desde casos asintomáticos hasta casos crónicos graves que pueden involucrar problemas cardiacos, gastrointestinales o ambos (3).…”

unclassified

Análisis por LSSP-PCR de la variabilidad genética de Trypanosoma cruzi en sangre y órganos de ratones.

Mejía

Triana-Chávez

2005

biomedica

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Introducción. La enfermedad de Chagas, causada por Trypanosoma cruzi, presenta un curso clínico variable que oscila desde casos asintomáticos a casos crónicos. T. cruzi tiene una estructura clonal y las cepas infectivas son a menudo multiclonales. La variabilidad genética de T. cruzi puede ser un determinante para el tropismo diferencial a tejidos y, consecuentemente, para las formas clínicas de la enfermedad. Objetivo. Caracterizar genéticamente los parásitos de sangre y órganos de ratones infectados con dos cepas colombianas de T. cruzi. Materiales y métodos. Se infectaron ratones con dos cepas colombianas de T. cruzi con el fin de determinar la infección en sangre y órganos. Para esto, se evaluó la sensibilidad de tres marcadores moleculares diferentes, y se determinó la variabilidad genética de los clones por la técnica de reacción en cadena de la polimerasa de baja astringencia con un único iniciador específico (LSSP-PCR), utilizando el marcador del ADN del cinetoplasto (kADN). Los perfiles de bandas obtenidos con la LSSP-PCR se analizaron por el método de neighbor-joining. Resultados y conclusiones. Nuestros resultados confirmaron la presencia de los dos grupos de T. cruzi en las cepas y el carácter policlonal de éstas. El marcador más sensible fue el kADN y el órgano más afectado, el corazón. Se encontraron diferencias genéticas entre los clones presentes en la sangre y los órganos de los ratones infectados. En conclusión, estos resultados apoyan el uso de la LSSP-PCR para el entendimiento de la epidemiología de la enfermedad de Chagas. Palabras clave: Trypanosoma cruzi, variación genética, LSSP-PCR, ADN del cinetoplasto, Enfermedad de ChagaGenetic variability of Trypanosoma cruzi in blood and organs of infected mice determined by LSSP-PCR Background. Chagas disease, caused by Trypanosoma cruzi, has a variable clinical course, ranging from asymptomatic infection to chronic disease. Trypanosoma cruzi has a clonal population structure, although infecting strains are often multiclonal. Genetic variability of T. cruzi may be one of the determinant factors in differential tissue tropism and consequently of the clinical forms of the disease. Objective. To examine this possibility, mice were infected with two Colombian T. cruzi strains to determine the distribution of genetic variants in blood and organs. The sensitivity of three molecular markers was evaluated, and the genetic variability of the clones was determined. The latter was attained by means of low-stringency single specific primer polymerase chain reaction (LSSP-PCR) using a kinetoplast DNA (kDNA) marker. The kDNA signatures obtained with the LSSP-PCR were analyzed by neighbor-joining. Results and conclusion. The presence of the two lineages of T. cruzi was confirmed, as well as the multiclonal character of the two strains. The most sensitive marker was the kDNA. The most affected organ was the heart, which showed the greatest number of positive results with the three markers. Genetic differences were noted between the clones from the blood and organs...

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Nova tripanozomiaze humana: estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n. sp., ajente etiolojico de nova entidade morbida do homem

Cited by 1,317 publications

References 0 publications

Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil

O processo de avaliação em ciência e a indicação de Carlos Chagas ao prêmio Nobel de Fisiologia ou Medicina

Expanding the knowledge of the chemical structure of glycoconjugates from Trypanosoma cruzi TcI genotype. Contribution to taxonomic studies

Match-making for posaconazole through systems thinking

Análisis por LSSP-PCR de la variabilidad genética de Trypanosoma cruzi en sangre y órganos de ratones.

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