Non-covalent Functionalization of Carbon Nanotubes for Efficient Gene Delivery

Burlaka, O. M.; Yemets, А. І.; Pirko, Ya. V.; Blume, Ya. B.

doi:10.1007/978-3-319-30737-4_30

Cited by 6 publications

(3 citation statements)

References 151 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanoholes were developed on the plant cell wall, which facilitated the delivery of biomolecules across the cell wall [ 187 ]. For successful DNA transport in plant cells, an optimized amount of non-covalently functionalized SWCNTs and MWCNTs is important [ 188 ]. Yellow fluorescent protein signals indicated that the SWCNTs were able to successfully permeate plant cell walls and transport the reporter gene inside cells from a variety of plant tissues, whereas the MWCNTs could only enter protoplast cell membranes.…”

Section: Cnts For Genetic Materials or Drug Deliverymentioning

confidence: 99%

Engineering plants with carbon nanotubes: a sustainable agriculture approach

et al. 2022

View full text Add to dashboard Cite

Sustainable agriculture is an important conception to meet the growing food demand of the global population. The increased need for adequate and safe food, as well as the ongoing ecological destruction associated with conventional agriculture practices are key global challenges. Nanomaterials are being developed in the agriculture sector to improve the growth and protection of crops. Among the various engineered nanomaterials, carbon nanotubes (CNTs) are one of the most promising carbon-based nanomaterials owing to their attractive physiochemical properties such as small size, high surface area, and superior mechanical and thermal strength, offering better opportunities for agriculture sector applications. This review provides basic information about CNTs, including their history; classification; and electrical, thermal, and mechanical properties, with a focus on their applications in the agriculture field. Furthermore, the mechanisms of the uptake and translocation of CNTs in plants and their defense mechanisms against environmental stresses are discussed. Finally, the major shortcomings, threats, and challenges of CNTs are assessed to provide a broad and clear view of the potential and future directions for CNT-based agriculture applications to achieve the goal of sustainability.

show abstract

Section: Cnts For Genetic Materials or Drug Deliverymentioning

confidence: 99%

Engineering plants with carbon nanotubes: a sustainable agriculture approach

et al. 2022

View full text Add to dashboard Cite

show abstract

“…This type of functionalization does not change the special properties of CNTs. For biological applications, the substances used for noncovalent functionalization should have properties like being biocompatible and nontoxic, stable attachment (Burlaka et al, 2016). Functionalization of CNTs help in reducing its toxicity to plant and even mammalian cells.…”

Section: Functionalization Of Cntsmentioning

confidence: 99%

Role of carbon nanotubes (CNTs) in transgenic plant development

Pawar,

Anumalla,

Sharma

2023

Biotech & Bioengineering

View full text Add to dashboard Cite

Carbon nanotubes (CNTs) are nanostructures, allotropes of carbon which are made up of graphene sheets wrapped around it forming cylindrical structures. CNTs have been regarded to have interesting and attractive physical and chemical properties and have been tremendously used in genetic engineering. Understanding the role of CNTs in development of transgenic plants, review of research papers in the field was done. CNTs are classified into two categories: the single‐walled and multiwalled (MWCNTs) structures. They are valuable vectors in various biomedicine fields such as Gene delivery, Drug delivery, Immunotherapy, Tissue engineering, and Biomedical imaging and also, they deliver the DNA without damaging the cells. Based on recent studies, the functionalization of CNTs when combined with some other suitable molecules can drastically subside their toxic effects. Having unique properties such as small size, larger surface area is useful in delivering DNA into mammalian cells as well. Modifications in CNTs can make nucleic acids adhere to them even more efficiently. Also, MWCNTs are crucial in delivery DNA into the cytoplasm. Based on other methods, the CNTs‐DNA are a preferred choice and the inclination toward double‐stranded DNA is used over single‐stranded DNA in gene delivery shows effective results. The only downside of CNTs is that they are hydrophobic and are difficult to form an aqueous solution, thus limiting their applicability. This review will aid you in comprehending useful knowledge related to a general overview of topics related to CNTs.

show abstract

“…Burlaka et al 155 used single-walled and multiwalled carbon nanotubes for the genetic transformation of N. tabacum L. protoplasts, callus cells, and leaf explants. The concentration of carbon nanotubes is one factor in improving the efficiency of gene delivery.…”

Section: ■ Transport and Delivery Systemsmentioning

confidence: 99%

Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

Mujtaba

Wang

Carvalho

et al. 2021

ACS Agric. Sci. Technol.

View full text Add to dashboard Cite

Current trends in plant genetic transformation technologies, i.e., designing and applying molecules like miRNA, RNAi, and CRISPR-Cas, largely enable researchers to target specific sites in the plant genome to avert the growing biotic and abiotic threats to plants. However, the delivery of these molecules through conventional techniques brings an array of drawbacks such as low efficiency due to the cell wall barrier, tissue damage that leads to browning or necrosis, degradation of these biomolecules by physiological conditions (high temperature, harsh pH, and light), and plant-specific protocols. The advancements in nanotechnology offer an excellent alternative for the safe and highly efficient delivery of biomolecules such as miRNA, CRISPR-Cas, and RNAi without damaging the plant tissues. Nanoparticle (polymeric, metallic, magnetic, silica, carbon, etc.)-based delivery of biomolecules can be efficiently utilized especially for plant protection applications. Herein, we present a comprehensive overview of current trends (with a focus on the previous five years) in nanoparticle-based delivery of miRNA, RNAi, CRISPR-Cas and simillar biomolecules for plant protection applications. In addition, a future perspective focuses on the research gaps and unexplored potentials of nanoparticles for the delivery of biomolecules.

show abstract

Non-covalent Functionalization of Carbon Nanotubes for Efficient Gene Delivery

Cited by 6 publications

References 151 publications

Engineering plants with carbon nanotubes: a sustainable agriculture approach

Engineering plants with carbon nanotubes: a sustainable agriculture approach

Role of carbon nanotubes (CNTs) in transgenic plant development

Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

Contact Info

Product

Resources

About