Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

Mujtaba, Muhammad; Wang, Depeng; Carvalho, Lucas Bragança; Oliveira, Jhones Luiz de; Pereira, Anderson do Espírito Santo; Sharif, Rahat; Jogaiah, Sudisha; Paidi, Murali Krishna; Wang, Lichen; Ali, Qasid; Fraceto, Leonardo Fernandes

doi:10.1021/acsagscitech.1c00146

Cited by 42 publications

(34 citation statements)

References 170 publications

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“…Nano-delivery systems have exploited the high degree of control over morphology and surface functionalized groups, the diverse conjugation chemistries available for cargo conjugation, and the permeability through plant tissues to overcome the biological barriers [ 24 ]. These advancements in nanotechnology have suggested a great potential for the safe and highly efficient delivery of biomolecules such as miRNA, CRISPR-Cas, and RNAi into plant cells, and it is expected to be a promising method for overcoming the limitations of miRNA delivery [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize

et al. 2022

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Background MicroRNA (miRNA) plays vital roles in the regulation of both plant architecture and stress resistance through cleavage or translation inhibition of the target messenger RNAs (mRNAs). However, miRNA-induced gene silencing remains a major challenge in vivo due to the low delivery efficiency and instability of miRNA, thus an efficient and simple method is urgently needed for miRNA transformation. Previous researches have constructed a star polycation (SPc)-mediated transdermal double-stranded RNA (dsRNA) delivery system, achieving efficient dsRNA delivery and gene silencing in insect pests. Results Here, we tested SPc-based platform for direct delivery of double-stranded precursor miRNA (ds-MIRNA) into protoplasts and plants. The results showed that SPc could assemble with ds-MIRNA through electrostatic interaction to form nano-sized ds-MIRNA/SPc complex. The complex could penetrate the root cortex and be systematically transported through the vascular tissue in seedlings of Arabidopsis and maize. Meanwhile, the complex could up-regulate the expression of endocytosis-related genes in both protoplasts and plants to promote the cellular uptake. Furthermore, the SPc-delivered ds-MIRNA could efficiently increase mature miRNA amount to suppress the target gene expression, and the similar phenotypes of Arabidopsis and maize were observed compared to the transgenic plants overexpressing miRNA. Conclusion To our knowledge, we report the first construction and application of star polycation nanocarrier-based platform for miRNA delivery in plants, which explores a new enable approach of plant biotechnology with efficient transformation for agricultural application. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize

et al. 2022

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“…Nanotechnology has made significant advances during the last decade especially in relation to plant genetic engineering. Recent reports have highlighted the importance of nanomaterials such as carbon dots, carbon nanotubes (CNTs), and mesoporous silicon nanoparticles (MSNs) to deliver biomolecules such as DNA, RNA, RNPs, and proteins [ 99 ]. In plants, nanoparticle-mediated administration of DNA and proteins into nuclear and chloroplast genomes has been achieved [ 100 , 101 ].…”

Section: Approaches To Applying Crispr/cas9 System In Plantsmentioning

confidence: 99%

CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops

et al. 2022

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Demand of flowers is increasing with time worldwide. Floriculture has become one of the most important commercial trades in agriculture. Although traditional breeding methods like hybridization and mutation breeding have contributed significantly to the development of important flower varieties, flower production and quality of flowers can be significantly improved by employing modern breeding approaches. Novel traits of significance have interest to consumers and producers, such as fragrance, new floral color, change in floral architecture and morphology, vase life, aroma, and resistance to biotic and abiotic stresses, have been introduced by genetic manipulation. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has recently emerged as a powerful genome-editing tool for accurately changing DNA sequences at specific locations. It provides excellent means of genetically improving floricultural crops. CRISPR/Cas system has been utilized in gene editing in horticultural cops. There are few reports on the utilization of the CRISPR/Cas9 system in flowers. The current review summarizes the research work done by employing the CRISPR/Cas9 system in floricultural crops including improvement in flowering traits such as color modification, prolonging the shelf life of flowers, flower initiation, and development, changes in color of ornamental foliage by genome editing. CRISPR/Cas9 gene editing could be useful in developing novel cultivars with higher fragrance and enhanced essential oil and many other useful traits. The present review also highlights the basic mechanism and key components involved in the CRISPR/Cas9 system.

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“…Other than delivering RNAs into plants, NPs have also been utilized for DNA delivery (Mujtaba et al 2021). DNA-loaded mesoporous silica nanoparticles (MSN), single-walled carbon nanotubes (SWNT), and LDH may be internalized into intact plant cells without requiring strong mechanical aids (Bao et al 2016;Kwak et al 2019;Chang et al 2013).…”

Section: Exogenous Application Of Rna-based Approaches For Plant Prot...mentioning

confidence: 99%

Perspectives on plant virus diseases in a climate change scenario of elevated temperatures

et al. 2022

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Global food production is at risk from many abiotic and biotic stresses and can be affected by multiple stresses simultaneously. Virus diseases damage cultivated plants and decrease the marketable quality of produce. Importantly, the progression of virus diseases is strongly affected by changing climate conditions. Among climate-changing variables, temperature increase is viewed as an important factor that affects virus epidemics, which may in turn require more efficient disease management. In this review, we discuss the effect of elevated temperature on virus epidemics at both macro- and micro-climatic levels. This includes the temperature effects on virus spread both within and between host plants. Furthermore, we focus on the involvement of molecular mechanisms associated with temperature effects on plant defence to viruses in both susceptible and resistant plants. Considering various mechanisms proposed in different pathosystems, we also offer a view of the possible opportunities provided by RNA -based technologies for virus control at elevated temperatures. Recently, the potential of these technologies for topical field applications has been strengthened through a combination of genetically modified (GM)-free delivery nanoplatforms. This approach represents a promising and important climate-resilient substitute to conventional strategies for managing plant virus diseases under global warming scenarios. In this context, we discuss the knowledge gaps in the research of temperature effects on plant-virus interactions and limitations of RNA-based emerging technologies, which should be addressed in future studies.

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Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

Cited by 42 publications

References 170 publications

Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize

Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize

CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops

Perspectives on plant virus diseases in a climate change scenario of elevated temperatures

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