Nitric oxide (NO) scavenging capacity of natural antioxidants

Sueishi, Yoshimi; Hori, Masashi; Kita, Masakazu; Kotake, Yashige

doi:10.1016/j.foodchem.2011.05.036

Cited by 52 publications

(23 citation statements)

References 28 publications

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“…It is a diffusible free radical that plays many roles as an effector molecule in diverse biological systems including neuronal messenger, vasodilation and antimicrobial and antitumor activities [31]. However, in pathological situations NO is also known to injure cells and tissues at relatively high concentrations [32]. …”

Section: Resultsmentioning

confidence: 99%

Evaluation of in vitro and in vivo Biological Activities of Cheilanthes albomarginata Clarke

Lamichhane

Kim

Poudel

et al. 2014

BMC Complement Altern Med

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BackgroundThe Cheilanthes albomarginata Clarke (CA), a fern belonging to Pteridaceae family, is found mainly in India, Nepal, Pakistan and Bhutan at an altitude of 1300–2700 m. It grows mostly in the rock crevices on slopes. Juice from the rhizome of CA has been used to treat peptic ulcer. In this study, the biological activities (antioxidant, anti-inflammatory, anti-adipogenic and anti-obesity) of the extracts of CA were investigated. The total phenolic content of each extract was quantified. This is the first report regarding the study of biological activities on CA.MethodsIn the current study, the crude methanol and fractionated extract of the aerial part of CA were investigated for the antioxidant tests which were namely DPPH assay, hydrogen peroxide scavenging assay and nitrite scavenging assay. Their phenolic contents were measured by the Folin-Ciocalteu’s method.In vitro anti-inflammatory and anti-adipogenic assays were evaluated against the RAW 264.7 macrophage cells and 3 T3-L1 cells respectively. The crude methanol extract and phenolic fraction (combination of ethyl acetate and butanol fraction) were studied for the in vivo anti-obesity test using male Sprague Dawley rats.ResultsThe ethyl acetate fraction showed the strongest DPPH radical scavenging (82.54 ± 0.48%), hydrogen peroxide scavenging (3.41 ± 0.21 mg/ml) and nitrite scavenging activity (61.39%). The highest phenolic content was found in the ethyl acetate fraction followed by the butanol fraction. The ethyl acetate fraction showed the highest in vitro anti-inflammatory and anti-adipogenic activities. From the in vivo study on rats, the crude methanol extract and phenolic fraction showed plasma triglyceride lowering activity as well as reduction of weight of adipose tissue in high fat diet induced obese rats.ConclusionThe current study suggests that the ethyl acetate and butanol extracts of CA are potential source for antioxidant, anti-inflammatory and anti-adipogenic remedies. In addition to that the results of in vivo studies evidenced the possibility of CA as a source of anti-obesity drug remedies.

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Section: Resultsmentioning

confidence: 99%

Evaluation of in vitro and in vivo Biological Activities of Cheilanthes albomarginata Clarke

Lamichhane

Kim

Poudel

et al. 2014

BMC Complement Altern Med

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“…In the present study, average serum UA quantified in the first trimester of Study results showed positive correlation between UA and ADMA with no statistical significance in the first trimester of pregnancy. Both maternal serum UA and ADMA levels are reduced in normal pregnancy but increase as gestational age increases [55] [56]. In early pregnancy, the reduction in UA levels is due to the effects of estrogen, expanded blood volume and increased glomerular filtration rate.…”

Section: Discussionmentioning

confidence: 99%

Maternal Serum Biochemical Indicators of Trophoblastic Cell and Endothelial Function at First Trimester of Pregnancy

Gadde¹,

Dayanand²,

Sheela³

2018

OJOG

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Background: Preeclampsia is a worldwide pregnancy complication, and early identification of patients with an increased risk is one of the key goals in obstetrics. First trimester screening is crucial over the second trimester for understanding the early onset of the disorder, with basal levels of the biochemical parameters associated with the underlying placentation process. Objective: The study aims to assess the levels of serum biochemical markers in pregnant women at first trimester, to evaluate statistical significance and correlation of the values in support of trophoblastic cell integrity, endothelial function and oxidative stress. Materials and Methods: A longitudinal study was conducted on 86 pregnant women of age group 20 -35 years, Primigravida with singleton pregnancy who visited prenatal check up between 11 -13 weeks of gestation. Maternal sera was collected for screening Placental protein 13 (PP13), Caspase 3, Asymmetric dimethylarginine (ADMA), Nitric oxide (NO) by ELISA. Xanthine oxidase (XO) activity was assayed spectrophotometrically. Calcium and Uric acid (UA) were measured by dry chemistry analyser. Results: The mean ± SD values for mean arterial pressure (MAP) are 108.4 ± 18.9, UA 2.01 ± 0.85, Total oxidant status (TOS) 12.83 ± 5.17, Total antioxidant capacity (TAC) 24.

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“…Over-accumulation of NO and some of it adducts can cause nitrosative stress, causing direct or indirect molecular damage, which can reduce abiotic stress tolerance [157]. GSH is a poor NO-scavenger [158], and GSNO is often regarded as a mobile source of NO and also a reactive nitrogen species (RNS) itself [159]. However, GSNO may mediate intracellular NO scavenging in a reaction that produces GSSG and NO 3 − [160].…”

Section: Glutathionementioning

confidence: 99%

A Central Role for Thiols in Plant Tolerance to Abiotic Stress

Zagorchev

Seal

Kranner

et al. 2013

IJMS

346

154

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Abiotic stress poses major problems to agriculture and increasing efforts are being made to understand plant stress response and tolerance mechanisms and to develop new tools that underpin successful agriculture. However, the molecular mechanisms of plant stress tolerance are not fully understood, and the data available is incomplete and sometimes contradictory. Here, we review the significance of protein and non-protein thiol compounds in relation to plant tolerance of abiotic stress. First, the roles of the amino acids cysteine and methionine, are discussed, followed by an extensive discussion of the low-molecular-weight tripeptide, thiol glutathione, which plays a central part in plant stress response and oxidative signalling and of glutathione-related enzymes, including those involved in the biosynthesis of non-protein thiol compounds. Special attention is given to the glutathione redox state, to phytochelatins and to the role of glutathione in the regulation of the cell cycle. The protein thiol section focuses on glutaredoxins and thioredoxins, proteins with oxidoreductase activity, which are involved in protein glutathionylation. The review concludes with a brief overview of and future perspectives for the involvement of plant thiols in abiotic stress tolerance.

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Nitric oxide (NO) scavenging capacity of natural antioxidants

Cited by 52 publications

References 28 publications

Evaluation of in vitro and in vivo Biological Activities of Cheilanthes albomarginata Clarke

Evaluation of in vitro and in vivo Biological Activities of Cheilanthes albomarginata Clarke

Maternal Serum Biochemical Indicators of Trophoblastic Cell and Endothelial Function at First Trimester of Pregnancy

A Central Role for Thiols in Plant Tolerance to Abiotic Stress

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