NIR-IIb excitable bright polymer dots with deep-red emission for in vivo through-skull three-photon fluorescence bioimaging

Alifu, Nuernisha; Zebibula, Abudureheman; Zhang, Hequn; Ni, Huwei; Zhu, Liang; Wang, Xi; Wang, Yalun; Zhang, Xueliang; Wu, Changfeng; Qian, Jun

doi:10.1007/s12274-020-2902-x

Cited by 27 publications

(18 citation statements)

References 31 publications

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“…Recently, NIR-IIb spectral region has been verified as an excellent optical tissue window for vessel imaging in vivo. Due to the reduced light scattering [46,47] and restrained autofluorescence [12] , the tertiary branches of uterine vessels in the unilateral uterus were vividly presented under the excitation of 793 nm CW laser (~80 mW cm -2 ) after intravenous injection of OTPA-BBT dots (1 mg mL -1 , 200 μ L) ( Figure 2B). The analysis of uterine angiography quality was presented in Figure 2C Figure S11, the excretion of OTPA-BBT dots could be confirmed, which is consistent with the conclusion in our previous work and the excretion can be attributed to the long aliphatic chains.…”

Section: The Uterine Angiography In Nir-iib Windowmentioning

confidence: 99%

Aggregation-induced emission dots assisted non-invasive fluorescence hysterography in near-infrared IIb window

Ying

Feng

et al. 2021

Preprint

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Uterine diseases seriously threaten the physical and mental health of women. The main principle, when clinicians adopt examinations, is to achieve efficient diagnosis without negative effect on the physical function including fertility. Hysterography in near-infrared (NIR) IIb window (1500-1700 nm) presents perceptibly enhanced signal to background ratio (SBR) and higher penetration capability compared with those beyond 1000 nm and 1300 nm, but lays down high requirements for the biosafety of fluorophores at the same time. Assisted by the biologically excretable aggregation-induced emission (AIE) dots, non-invasive NIR-IIb fluorescence hysterography visualized typical Y-shaped uteruses, real-time uterine peristalsis or the uterine lesions (mimetic disease statuses in clinic) in mouse models. Significantly, after intrauterine perfusion, the reproductive capacity was unimpaired via fertility assessment and histological analysis. This work could inspire some new ideas for non-invasive clinical diagnosis of uterine diseases and effectively promote the clinical translation of AIE dots.

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Section: The Uterine Angiography In Nir-iib Windowmentioning

confidence: 99%

Aggregation-induced emission dots assisted non-invasive fluorescence hysterography in near-infrared IIb window

Ying

Feng

et al. 2021

Preprint

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“…In order to ameliorate the penetration depth for deep‐tissue brain study, Alifu et al. introduced NIR‐IIb excitable Pdots with three‐photon fluorescence (3PF, three photons with lower energy are absorbed and one photon with higher energy is emitted) brightness and deep‐red emission [23] . Wherein they designed Pdots by packing different semiconducting polymers together to facilitate the interparticle energy transfer from poly(fluorene‐alt‐benzothiadiazole) (PFBT) (donor, light‐harvesting unit) and polyfluorenyldithienylbenzothiadiazol (PF−DBT5, red‐emission) (acceptor) polymers (Figure 2) with the assistance of PS−PEG−COOH.…”

Section: Deep‐tissue Fluorescence Imagingmentioning

confidence: 99%

“…Dose: [21] pNIR-4 750 1040 100 2.24 -In vivo delineation blood-vessels with enhanced clarity. [22] P-Pdot 600 1550 28 56 -In vivo through-skull imaging in live mice [23] TTQÀ 2TC NP 1064 900-1400 130 0.03 8,9.5 Dose: 1 mg/ml [24] PttcÀ SeBTaÀ NIR 1380 1082-1290 1300-1400 73 0.05 2.32 In vivo through-skull imaging in live mice.…”

Section: Deep-tissue Fluorescence Imagingmentioning

confidence: 99%

“…In order to ameliorate the penetration depth for deep-tissue brain study, Alifu et al introduced NIR-IIb excitable Pdots with three-photon fluorescence (3PF, three photons with lower energy are absorbed and one photon with higher energy is emitted) brightness and deep-red emission. [23] Wherein they designed Pdots by packing different semiconducting polymers together to facilitate the interparticle energy transfer from poly (fluorene-alt-benzothiadiazole) (PFBT) (donor, light-harvesting unit) and polyfluorenyldithienylbenzothiadiazol (PFÀ DBT5, redemission) (acceptor) polymers (Figure 2) with the assistance of PSÀ PEGÀ COOH. The resulting Pdots (P-Pdot) were optimized at a weight ratio of 0.6 (PFÀ DBT5/PFBT) to produce strong fluorescence caused by the acceptor moiety, which completely quenched the fluorescence of the donor (PFBT), thereby displaying 3PF absorption peak at~645 nm with a QY of~56% in aqueous solution under 1550 nm laser excitation.…”

Section: Deep-tissue Fluorescence Imagingmentioning

confidence: 99%

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Near‐Infrared‐II Semiconducting Polymer Dots for Deep‐tissue Fluorescence Imaging

Gupta

Chan

Saha

et al. 2020

Chemistry An Asian Journal

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Fluorescence imaging, particularly in the NIR-II region (1000-1700 nm), has become an unprecedented tool for deep-tissue in vivo imaging. Among the fluorescent nanoprobes, semiconducting polymer nanoparticles (Pdots) appear to be a promising agent because of their tunable optical and photophysical properties, ultrahigh brightness, minimal autofluorescence, narrow-size distribution, and low cytotoxicity. This review elucidates the recent advances in Pdots for deep-tissue fluorescence imaging and the facing future translation to clinical use.

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“…However, scattering and auto uorescence limit imaging depth and clarity in traditional uorescence imaging based on the visible (400-760 nm) and the near-infrared I (NIR-I, 760-900 nm) spectral regions [9]. Fluorescence imaging in the near-infrared II (NIR-II, 900-1700 nm) spectral region has become a powerful tool for precise diagnosis and treatment of cancers [10][11][12][13]. Near-infrared-II uorescence imaging offers deep tissue penetration and high spatial resolution due to extremely low light scattering in biological samples [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

A Novel TMTP1-modified Theranostic Nanoplatform for Targeted in Vivo NIR-II Fluorescence Imaging-guided Chemotherapy of Cervical Cancer

Alifu

Zhu

et al. 2021

Preprint

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BackgroundNear-infrared II (NIR-II, 900-1700 nm) fluorescence bioimaging with advantages of good biosafety, excellent spatial resolution, high sensitivity and contrast, has attracted great attentions in biomedical research fields. However, most nanoprobes used for NIR-II fluorescence imaging have poor tumor-targeting ability and therapeutic efficiency. To overcome these limitations, a novel NIR-II-emissive theranostic nanoplatform for imaging and treatment of cervical cancer was designed and prepared. The NIR-II-emissive dye IR-783 and chemotherapy drug doxorubicin (DOX) were encapsulated into liposomes, and the tumor-targeting peptide TMTP1 was conjugated to the surface of the liposomes to form IR-783-DOX-TMTP1 nanoparticles (NPs) via self-assembly methods.ResultsThe IR-783-DOX-TMTP1 NPs showed strong NIR-II emission, excellent biocompatibility, a long lifetime, and low toxicity. Further, high-definition NIR-II fluorescence microscopy images of ear blood vessels and intratumor blood vessels were obtained from IR-783-DOX-TMTP1 NPs-stained mice with high spatial resolution under 808 nm laser excitation. Moreover, IR-783-DOX-TMTP1 NPs showed strong tumor targeting ability and high efficiently chemotherapeutic character towards cervical tumors. ConclusionsThe novel targeting and NIR-II-emissive IR-783-DOX-TMTP1 NPs have potential in diagnosis and therapy for cervical cancer.

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NIR-IIb excitable bright polymer dots with deep-red emission for in vivo through-skull three-photon fluorescence bioimaging

Cited by 27 publications

References 31 publications

Aggregation-induced emission dots assisted non-invasive fluorescence hysterography in near-infrared IIb window

Aggregation-induced emission dots assisted non-invasive fluorescence hysterography in near-infrared IIb window

Near‐Infrared‐II Semiconducting Polymer Dots for Deep‐tissue Fluorescence Imaging

A Novel TMTP1-modified Theranostic Nanoplatform for Targeted in Vivo NIR-II Fluorescence Imaging-guided Chemotherapy of Cervical Cancer

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