Deep‐penetration fluorescence imaging in the second near‐infrared (NIR‐II) window heralds a new era of clinical surgery, in which high‐resolution vascular/lymphatic anatomy and detailed cancerous tissues can be visualized in real time. Described here is a series of polymethine‐based semiconducting polymers with intrinsic emission maxima in the NIR‐IIa (1300–1400 nm) window and absorption maxima ranging from 1082 to 1290 nm. These polymers were prepared as semiconducting polymer dots (Pdots) in aqueous solutions with fluorescence quantum yields of 0.05–0.18 %, and they demonstrate promising applications in noninvasive through‐skull brain imaging in live mice with remarkable spatial resolution as well as signal‐to‐background contrast. This study offers a platform for future design of NIR‐IIa or even NIR‐IIb emitting Pdots.

There have been enormous efforts for developing the next generations of fluorometric lateral flow immunochromatographic strip (ICTS) owing to the great advances in fluorescent materials in these years. Here we developed one type of fluorometric ICTS based on ultrabright semiconducting polymer dots (Pdots) in which the traffic light-like signals were created by energy transfer depending on the target concentration. This platform was successfully applied for qualitatively rapid screening and quantitatively precise analysis of prostate-specific antigen (PSA) in 10 min from merely one drop of the whole blood sample. This FRET-created traffic light ICTS possesses excellent specificity and an outstanding detection sensitivity of 0.32 ng/mL for PSA. Moreover, we conducted proof-of-concept experiments to demonstrate its potential for multiplexed detection of cancer biomarkers at the same time in an individual test strip by taking advantage of the traffic light signals. To the best of our knowledge, it is the first model of a traffic light-like immunoassay test strip based on Pdots with multiplexing ability. These results would pave an avenue for designing the next generation of point-of-care diagnostics.

Photolithographically generated patterns have been created on immobilized CdSe QD thin films by fine-tuning their optical properties (intensity and emission wavelength) postsynthetically. These optically modified QDs show enhanced selectivity for binding of different ligands, affording the ability to fabricate optically reconfigurable surfaces for display or sensing applications. The patterns may be readily generated with any typical optical lithographic approach.

In recent years, semiconducting polymer dots (Pdots) have attracted enormous attention in applications from fundamental analytical detection to advanced deep-tissue bioimaging due to their ultrahigh fluorescence brightness with excellent photostability and minimal cytotoxicity. Pdots have therefore been widely adopted for a variety types of molecular sensing for analytical detection. More importantly, the recent development of Pdots for use in the optical window between 1000 and 1700 nm, popularly known as the "second near-infrared window" (NIR-II), has emerged as a class of optical transparent imaging technology in the living body. The advantages of the NIR-II region over the traditional NIR-I (700−900 nm) window in fluorescence imaging originate from the reduced autofluorescence, minimal absorption and scattering of light, and improved penetration depths to yield high spatiotemporal images for biological tissues. Herein, we discuss and summarize the recent developments of Pdots employed for analytical detection and NIR-II fluorescence imaging. Starting with their preparation, the recent developments for targeting various analytes are then highlighted. After that, the importance of and latest progress in NIR-II fluorescence imaging using Pdots are reported. Finally, perspectives and challenges associated with the emergence of Pdots in different fields are given.

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Fluorescence probes emitting in the second near-infrared (NIR-II, 1000-1700 nm) window with the ability for deep-tissue imaging in mammals herald a new era in surgical methodology. However, the brightness of these NIR-II probes is still far from satisfactory due to their low fluorescence quantum yields (QYs), preventing the observation of high-resolution images such as whole-organ vascular networks in real time. Described here is the molecular engineering of a series of semiconducting polymer dots (Pdots) incorporated with aggregation-induced emission moieties to exhibit the QYs as high as 14% in the NIR-II window. Benefiting from the ultrahigh brightness, a 1400 nm long-pass filter is utilized to realize in vivo 3D tumor mapping in mice. To further understand how the geometrical and electron structures of the semiconducting polymers affect their optical properties, the in-depth and thorough density-functional theory calculations are performed to interpret the experimental results. This study lays the groundwork for further molecular design of highly bright NIR-II Pdots.

Photothermal therapy (PTT) techniques have been developed for years, while active endeavors have been continuously undertaken recently to enhance their efficiency by employing near-infrared light. Among the photothermal agents, semiconducting polymer dots (Pdots) appear to be a promising type of agent because of their large absorption coefficients, phenomenal photostability, tunable optical properties, and good biocompatibility. This review article systematically summarizes recent advances in Pdots for deep-tissue PTT and perspectives/challenges on the nascent field.

Fluorescence imaging, particularly in the NIR-II region (1000-1700 nm), has become an unprecedented tool for deep-tissue in vivo imaging. Among the fluorescent nanoprobes, semiconducting polymer nanoparticles (Pdots) appear to be a promising agent because of their tunable optical and photophysical properties, ultrahigh brightness, minimal autofluorescence, narrow-size distribution, and low cytotoxicity. This review elucidates the recent advances in Pdots for deep-tissue fluorescence imaging and the facing future translation to clinical use.