Aggregation-induced emission dots assisted non-invasive fluorescence hysterography in near-infrared IIb window

Abstract

Uterine diseases seriously threaten the physical and mental health of women. The main principle, when clinicians adopt examinations, is to achieve efficient diagnosis without negative effect on the physical function including fertility. Hysterography in near-infrared (NIR) IIb window (1500-1700 nm) presents perceptibly enhanced signal to background ratio (SBR) and higher penetration capability compared with those beyond 1000 nm and 1300 nm, but lays down high requirements for the biosafety of fluorophores at the same time. Assisted by the biologically excretable aggregation-induced emission (AIE) dots, non-invasive NIR-IIb fluorescence hysterography visualized typical Y-shaped uteruses, real-time uterine peristalsis or the uterine lesions (mimetic disease statuses in clinic) in mouse models. Significantly, after intrauterine perfusion, the reproductive capacity was unimpaired via fertility assessment and histological analysis. This work could inspire some new ideas for non-invasive clinical diagnosis of uterine diseases and effectively promote the clinical translation of AIE dots.

Fluorescence visualization of deep-buried hollow organs

“…The second near-infrared (NIR-II, 900-1880 nm) window gives a promising fix for deep-penetration biological imaging [1][2][3][4][5][6] . The vessels, lymph, tumor, etc., have been successfully visualized intravitally by virtue of NIR-II fluorescence imaging [7][8][9][10][11][12][13][14][15][16] . Besides the reduced photon scattering, the rising light absorption by water further restrain the scattering disturbance in tissues during NIR-II intravital imaging 17,18 .…”