Newly identified degradation products of ceftiofur and cephapirin impact the analytical approach for quantitative analysis of kidney

High resolution mass spectrometry in the identification of transformation products and metabolites from β-lactam antibiotics in thermally treated milk

“…The resulting hydroxy acid undergoes cyclization to the cephapirin lactone (PIR-4, m/z 364.0420) and the hydrolysis of the ␤-lactam ring in the latter yields PIR-6 (hydrolyzed cephapirin lactone, m/z 382.0526). These compounds, together with PIR-7 (methoxy desacetylcephapirin, m/z 396.0682) and PIR-8 (desacetylcephapirin methyl ester, m/z 396.0682), were detected previously in chicken muscle and bovine milk and kidney [17][18][19]. The formation of PIR-7 and PIR-8 can be tentatively rationalized as the degradation of PIR-5 affected by the sample preparation procedure, in which MeOH is used.…”

“…The limited number of papers published regarding antibiotics in matrices such as meat or milk constitutes a demonstration. Among them, the simulated degradation at physiological temperatures of the cephalosporins ceftiofur and cephapirin in kidney extract has been described [17]. TPs and metabolites of the ␤-lactam antibiotics amoxicillin, cephapirin and ceftiofur, have also been studied in chicken meat [18].…”

Quechers methodologies as an alternative to solid phase extraction (SPE) for the determination and characterization of residues of cephalosporins in beef muscle using LC–MS/MS

“…In general, the QuEChERS method seemed to provide slightly better results than the SPE counterpart, and recovery values were only lower than 80% for LEX. The PIR recovery of the SPE method was rather poor, probably due to a degradation underwent by this cephalosporin when was in contact for a long time with meat samples as some studies have described [37].…”

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

“…Issues pertaining to the lack of stability in milk and tissues of certain βLs has been reported in literature [39]. The degradation products of two cephalosporins, ceftiofur and cephapirin, in kidney extract and in acidic and basic solutions was described in recent work by Berendsen et al [277].…”

“…Hence, it is common to analyse diaminopoyrimidines together with SA [276][277][278][279][280][281]. Recent review papers by Wang et al [10] and Samanidou et al [318] provide an excellent overview of SAs in foodstuff of animal origin.…”