The assay of acetaminophen (APAP) and ascorbic acid (AA) was performed by liquid chromatography (LC) with amperometric detection at a screen printed carbon based electrode poised at + 0.6 V versus Ag pseudoreference electrode. A microparticle based conventional RP-C18 and a monolithic RP-C18 column were studied comparatively for the analytes separation using a mobile phase based on 0. 1 M phosphate buffer-methanol 91 : 9 (v/v) at pH 6.5. The linear calibration range for both compounds was comprised within 2 10 À7 -1 10 À4 M. Limit of detection (LOD) for APAP and AA was 2 10 À7 and 5 10 À7 M, respectively and the limit of quantification (LOQ) was 5. 10 À7 and 1 10 À6 M, respectively. Square wave voltammetry at a Nafion modified glassy carbon electrode was developed and applied to the determination of APAP in order to validate the results obtained by the monolithic LC method for drug formulations.
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