Aptamer-modified pencil graphite electrodes for the impedimetric determination of ochratoxin A

Gökçe, Gültekin; Aissa, Sondes Ben; Nemčeková, Katarína; Catanante, Gaëlle; Raouafi, Noureddine; Marty, Jean‐Louis

doi:10.1016/j.foodcont.2020.107271

Cited by 40 publications

(17 citation statements)

References 26 publications

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“…One of the most frequently encountered reaction strategies for the aptamer immobilization is the covalent bonding between the aptamer and the electrode surface via a carbodiimide-mediated reaction. The immobilization methods based on diazonium functionalized surfaces by means of electrochemical grafts have been widely used [ 33 , 34 , 35 , 36 , 37 , 38 , 39 ], to obtain stable functionalized conductive surfaces for the immobilization of biomolecules.…”

Section: Immobilization Chemistry Of Aptamer Onto the Electrode Sumentioning

confidence: 99%

Electrochemical Aptasensors: Current Status and Future Perspectives

Abd-Ellatief

2021

Diagnostics

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This article reviews the progress of diversity of electrochemical aptasensor for target analytes detection. The immobilization strategies of aptamers on an electrode surface are addressed. The aptasensors are also introduced in compliance with the assay platforms. Many electrochemical aptasensors are nearly identical to conventional immunochemical approaches, sandwich and competition assays using electroactive signaling moieties. Others are “signal-on” and “sign-off” aptasensors credited to the target binding-induced conformational change of aptamers. Label-free aptasensors are also highlighted. Furthermore, the aptasensors applied for clinically important biomarkers are emphasized.

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Section: Immobilization Chemistry Of Aptamer Onto the Electrode Sumentioning

confidence: 99%

Electrochemical Aptasensors: Current Status and Future Perspectives

Abd-Ellatief

2021

Diagnostics

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“…2 times) than the one obtained via Zn-mediated electrografting. 64 More recently, carboxyphenyl platforms, were exploited on gold and graphite by the Marty's group to prepare aptamer-based sensors respectively dedicated to the detection of ochratoxin A 65 and cadmium(II). 66 In both cases, in situ generation of the carboxyphenyl diazonium was exploited and the coupling reaction was implemented with a large excess of the EDC/NHS couple (i.e.…”

Section: Metal Cation Sensorsmentioning

confidence: 99%

“…Carbon 114 -Gold, 165 Gold, 20,336 carbon 21,300 ORR, 20 biofuel cells, 21 sugar sensing 300 Non enzymatic proteins (including peptides, amino acids and analogues) Amide coupling Carbon, 10,11,39,42,44,61,73,325,337,338 graphite-epoxy composite 13 gold, 9,10,12,[34][35][36]40,47 SWCNT, 53 Fe-core/cabon-shell NPs, 57 graphene, 54,339 AgNPs/SiO2/GO hybrid, 55 ITO, 37 boron-doped carbon nanowall 340 Immunosensing, 35- Nucleic acids (DNA, aptamers) Amide coupling Gold, 58,66,68 carbon, 59,63,64,344 graphene, 67,334 SPCE, pencil graphite electrode 65 Sensing, 334 DNA and protein detection, 58 DNA biosensing, 59,…”

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confidence: 99%

A post-functionalization toolbox for diazonium (electro)-grafted surfaces: review of the coupling methods

2021

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“…Moreover, impedance method is known for its signal instability due to the electrode to electrode and probe variations. Normalization of the impedance signals can be helpful to minimize this inconsistency in laboratory research (Gökçe et al., 2020). For commercialized impedimetric biosensors, the potential solutions include the better control of electrode manufacturing in mass production as well as self‐calibration against electrode variations during tests to ensure reproducible results.…”

Section: Biosensors With Different Transduces For Detection Of Salmonmentioning

confidence: 99%

Biosensors for rapid detection of Salmonella in food: A review

Shen

2020

Comp Rev Food Sci Food Safe

138

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Salmonella is one of the main causes of foodborne infectious diseases, posing a serious threat to public health. It can enter the food supply chain at various stages of production, processing, distribution, and marketing. High prevalence of Salmonella necessitates efficient and effective approaches for its identification, detection, and monitoring at an early stage. Because conventional methods based on plate counting and real‐time polymerase chain reaction are time‐consuming and laborious, novel rapid detection methods are urgently needed for in‐field and on‐line applications. Biosensors provide many advantages over conventional laboratory assays in terms of sensitivity, specificity, and accuracy, and show superiority in rapid response and potential portability. They are now recognized as promising alternative tools and one of the most on‐site applicable and end user–accessible methods for rapid detection. In recent years, we have witnessed a flourishing of studies in the development of robust and elaborate biosensors for detection of Salmonella in food. This review aims to provide a comprehensive overview on Salmonella biosensors by highlighting different signal‐transducing mechanisms (optical, electrochemical, piezoelectric, etc.) and critically analyzing its recent trends, particularly in combination with nanomaterials, microfluidics, portable instruments, and smartphones. Furthermore, current challenges are emphasized and future perspectives are discussed.

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Aptamer-modified pencil graphite electrodes for the impedimetric determination of ochratoxin A

Cited by 40 publications

References 26 publications

Electrochemical Aptasensors: Current Status and Future Perspectives

Electrochemical Aptasensors: Current Status and Future Perspectives

A post-functionalization toolbox for diazonium (electro)-grafted surfaces: review of the coupling methods

Biosensors for rapid detection of Salmonella in food: A review

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