Electrochemical Aptasensors: Current Status and Future Perspectives

Abd-Ellatief, Ragaa; Abd-Ellatief, Maha Ragaa

doi:10.3390/diagnostics11010104

Cited by 60 publications

(39 citation statements)

References 149 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrochemical aptasensors consist of two main components, the aptamer that serves as the bio-recognition element and the transducer that works to convert the aptamer binding events into a measurable signal [32]. The transducer can be further described as two main constituents: the electrode system and the electronic readout unit.…”

Section: Sensor Structure and Sensing Principlementioning

confidence: 99%

“…Traditionally employed labeled-antibody biosensing requires labels, such as enzymes and fluorescent or radioactive molecules to the targeted analyte, and thus is expensive, time-consuming, and frequently results in a reduced affinity of the analyte due to the non-site selective labeling [32,42]. In contrast, the electrochemical aptamer-based detection of thrombin could be label-free.…”

Section: Sensor Structure and Sensing Principlementioning

confidence: 99%

See 1 more Smart Citation

Nanomaterial-Based Label-Free Electrochemical Aptasensors for the Detection of Thrombin

2022

View full text Add to dashboard Cite

Thrombin plays a central role in hemostasis and its imbalances in coagulation can lead to various pathologies. It is of clinical significance to develop a fast and accurate method for the quantitative detection of thrombin. Electrochemical aptasensors have the capability of combining the specific selectivity from aptamers with the extraordinary sensitivity from electrochemical techniques and thus have attracted considerable attention for the trace-level detection of thrombin. Nanomaterials and nanostructures can further enhance the performance of thrombin aptasensors to achieve high sensitivity, selectivity, and antifouling functions. In highlighting these material merits and their impacts on sensor performance, this paper reviews the most recent advances in label-free electrochemical aptasensors for thrombin detection, with an emphasis on nanomaterials and nanostructures utilized in sensor design and fabrication. The performance, advantages, and limitations of those aptasensors are summarized and compared according to their material structures and compositions.

show abstract

Section: Sensor Structure and Sensing Principlementioning

confidence: 99%

Section: Sensor Structure and Sensing Principlementioning

confidence: 99%

Nanomaterial-Based Label-Free Electrochemical Aptasensors for the Detection of Thrombin

2022

View full text Add to dashboard Cite

show abstract

“…The prerequisite for obtaining a sandwich assay is that the analyte carries two epitopes, so that both receptors can bind simultaneously without affecting the two separate binding events [ 113 ]. The sandwich format commonly provides higher selectivity than single-receptor assays and can be assisted by a large variety of amplification methods [ 114 ].…”

Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

“… Schematic representation of sandwich-type structures employed in aptasensing assays. Reprinted from [ 114 ]. …”

Section: Figures Schemes and Tablesmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

View full text Add to dashboard Cite

Food safety monitoring assays based on synthetic recognition structures such as aptamers are receiving considerable attention due to their remarkable advantages in terms of their ability to bind to a wide range of target analytes, strong binding affinity, facile manufacturing, and cost-effectiveness. Although aptasensors for food monitoring are still in the development stage, the use of an electrochemical detection route, combined with the wide range of materials available as transducers and the proper immobilization strategy of the aptamer at the transducer surface, can lead to powerful analytical tools. In such a context, employing aryldiazonium salts for the surface derivatization of transducer electrodes serves as a simple, versatile and robust strategy to fine-tune the interface properties and to facilitate the convenient anchoring and stability of the aptamer. By summarizing the most important results disclosed in the last years, this article provides a comprehensive review that emphasizes the contribution of aryldiazonium chemistry in developing electrochemical aptasensors for food safety monitoring.

show abstract

“…Numerous electrochemical aptasensors have been developed so far for the detection of single analytes that are important from the points of view of (1) disease diagnostics [ 19 , 20 , 21 , 22 , 23 , 24 ], (2) food analysis [ 25 , 26 , 27 ], and (3) environmental monitoring [ 28 , 29 ]. Moreover, several review papers discussing current achievements and future perspectives in the area of electrochemical aptasensors have recently been presented [ 30 , 31 , 32 ]. Only a few examples are cited here.…”

Section: Introductionmentioning

confidence: 99%

Advances in Design Strategies of Multiplex Electrochemical Aptasensors

Grabowska

Hepel

Kurzątkowska-Adaszyńska

2021

Sensors

View full text Add to dashboard Cite

In recent years, the need for simple, fast, and economical detection of food and environmental contaminants, and the necessity to monitor biomarkers of different diseases have considerably accelerated the development of biosensor technology. However, designing biosensors capable of simultaneous determination of two or more analytes in a single measurement, for example on a single working electrode in single solution, is still a great challenge. On the other hand, such analysis offers many advantages compared to single analyte tests, such as cost per test, labor, throughput, and convenience. Because of the high sensitivity and scalability of the electrochemical detection systems on the one hand and the specificity of aptamers on the other, the electrochemical aptasensors are considered to be highly effective devices for simultaneous detection of multiple-target analytes. In this review, we describe and evaluate multi-label approaches based on (1) metal quantum dots and metal ions, (2) redox labels, and (3) enzyme labels. We focus on recently developed strategies for multiplex sensing using electrochemical aptasensors. Furthermore, we emphasize the use of different nanomaterials in the construction of these aptasensors. Based on examples from the existing literature, we highlight recent applications of multiplexed detection platforms in clinical diagnostics, food control, and environmental monitoring. Finally, we discuss the advantages and disadvantages of the aptasensors developed so far, and debate possible challenges and prospects.

show abstract

Electrochemical Aptasensors: Current Status and Future Perspectives

Cited by 60 publications

References 149 publications

Nanomaterial-Based Label-Free Electrochemical Aptasensors for the Detection of Thrombin

Nanomaterial-Based Label-Free Electrochemical Aptasensors for the Detection of Thrombin

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Advances in Design Strategies of Multiplex Electrochemical Aptasensors

Contact Info

Product

Resources

About