New Pathotype Nomenclature for Better Characterisation the Virulence and Diversity of Blumeria graminis f.sp. avenae Populations

Okoń, Sylwia; Cieplak, Magdalena; Kuzdraliński, Adam; Ociepa, Tomasz

doi:10.3390/agronomy11091852

Cited by 9 publications

(10 citation statements)

References 47 publications

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“…Avenae isolates collected from different parts of Europe. These differences suggested that the effectiveness of resistance genes varied from region to region, e.g., for many years, the Pm3 gene was considered ineffective in Poland [ 18 , 19 , 20 ] but in the current study its high resistance against isolates from Ireland and Finland was demonstrated. This research also showed that the virulence level of the population collected in Poland was very similar to that identified in previous years [ 18 , 19 ], indicating that changes in the pathogen population were slow.…”

Section: Discussionmentioning

confidence: 69%

“…Based on binary matrix, the analysed B. graminis f. sp. avenae isolates were classified into appropriate pathotypes according to the methodology described by Okoń et al [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…Studies on the virulence of oat powdery mildew have already been reported several times in the literature [ 18 , 19 , 20 ]. However, there is no information on research into the genetic diversity of B. graminis f. sp.…”

Section: Introductionmentioning

confidence: 99%

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Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe

Cieplak

Nucia

Ociepa

et al. 2022

Plants

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The structure and dynamics of changes in pathogen populations can be analysed by assessing the level of virulence and genetic diversity. The aim of the present study was to determine the diversity of Blumeria graminis f. sp. avenae populations. Diversity and virulence of B. graminis f. sp. avenae was assessed based on 80 single-spore isolates collected in different European countries such as Poland (40 isolates), Germany (10), Finland (10), Czech Republic (10) and Ireland (10) using ISSR (Inter-Simple Sequence Repeats) and SCoT (Start Codon Targeted) markers. This work demonstrated differences in virulence of B. graminis f. sp. avenae isolates sampled from different countries. Molecular analysis showed that both systems were useful for assessing genetic diversity, but ISSR markers were superior and generated more polymorphic products, as well as higher PIC and RP values. UPMGA and PCoA divided the isolates into groups corresponding with their geographical origin. In conclusion, the low level of genetic differentiation of the analysed isolates has suggested that the evolution of B. graminis f. sp. Avenae population is slow, and thus the evolutionary potential of the pathogen is low. This work paves the way for future studies on B. graminis f. sp. Avenae population structure and dynamics based on genetic variability.

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Section: Discussionmentioning

confidence: 69%

“…Based on binary matrix, the analysed B. graminis f. sp. avenae isolates were classified into appropriate pathotypes according to the methodology described by Okoń et al [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

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Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe

Cieplak

Nucia

Ociepa

et al. 2022

Plants

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“…Okoń et al [ 17 ] and Cieplak et al [ 33 ] showed that the resistance of the Pm12 gene is maintained at a level that allows for a satisfactory protection of plants against B. graminis f. sp. avenae .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the isolates differed in the level of virulence in relation to the described powdery mildew resistance genes. According to the nomenclature proposed by Okoń et al [ 17 ], the CZE isolate was classified as pathotype TBBB, which means that it was virulent to Pm1 , Pm3 , Pm6 and Pm3+8 genes. The CHR isolate was virulent to Pm1 , Pm3 , Pm6 , Pm3+8 , Pm9 and Pm11 and represented pathotype TBHB.…”

Section: Methodsmentioning

confidence: 99%

Chromosomal Location of Pm12—A Novel Powdery Mildew Resistance Gene from Avena sterilis

Ociepa

Okoń

2022

Genes

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Identification of new, effective disease resistance genes is a very important aspect of plant breeding. Also important is the precise location of individual loci and tagging them with DNA markers for marker assisted selection. The aim of the present study was identification of the molecular markers linked with Pm12, a new effective resistance gene to powdery mildew, and their location in the oat genome. The analysis was performed on 167 F2 individuals from a hybrid of Fuchs × CN67383, with the status of the locus in each individual verified by progeny test in F3. Segregation ratios confirmed the monogenic nature of resistance. Making use of the sequence data of DNA markers and the oat OT3098 v2 genome reference assembly, Pm12 is located on chromosome 7C. A comparison was also made with the reference consensus map, to which there are more reports of mapped genes to date. The mapping results suggest that Pm12 is located in the interval 103.8–111.7 cM on this map. No powdery mildew resistance locus has been identified in this region so far, suggesting that Avena sterilis CN67383 carries a novel locus offering effective resistance in oat breeding. The information included in the oat genome annotation allowed for the identification of candidate genes in the close region of the marker cluster for Pm12. This information may provide an interesting source of further analysis of the pathways of various genes in response to the stress of powdery mildew infection.

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Genetic mapping of the powdery mildew resistance gene Pm7 on oat chromosome 5D

et al. 2023

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Key message Three independent experiments with different genetic backgrounds mapped the resistance gene Pm7 in the oat genome to the distal part of the long arm of chromosome 5D. Abstract Resistance of oat to Blumeria graminis DC. f. sp. avenae is an important breeding goal in Central and Western Europe. In this study, the position of the effective and widely used resistance gene Pm7 in the oat genome was determined based on three independent experiments with different genetic backgrounds: genome-wide association mapping in a diverse set of inbred oat lines and binary phenotype mapping in two bi-parental populations. Powdery mildew resistance was assessed in the field as well as by detached leaf tests in the laboratory. Genotyping-by-sequencing was conducted to establish comprehensive genetic fingerprints for subsequent genetic mapping experiments. All three mapping approaches located the gene to the distal part of the long arm of chromosome 5D in the hexaploid oat genome sequences of OT3098 and ‘Sang.’ Markers from this region were homologous to a region of chromosome 2Ce of the C-genome species, Avena eriantha, the donor of Pm7, which appears to be the ancestral source of a translocated region on the hexaploid chromosome 5D.

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New Pathotype Nomenclature for Better Characterisation the Virulence and Diversity of Blumeria graminis f.sp. avenae Populations

Cited by 9 publications

References 47 publications

Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe

Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe

Chromosomal Location of Pm12—A Novel Powdery Mildew Resistance Gene from Avena sterilis

Genetic mapping of the powdery mildew resistance gene Pm7 on oat chromosome 5D

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