Genetic mapping of the powdery mildew resistance gene Pm7 on oat chromosome 5D

Abstract

Key message Three independent experiments with different genetic backgrounds mapped the resistance gene Pm7 in the oat genome to the distal part of the long arm of chromosome 5D. Abstract Resistance of oat to Blumeria graminis DC. f. sp. avenae is an important breeding goal in Central and Western Europe. In this study, the position of the effective and widely used resistance gene Pm7 in the oat genome was determined based on three independent experiments with different genetic backgrounds: genome-wide association mapping in a diverse set of inbred oat lines and binary phenotype mapping in two bi-parental populations. Powdery mildew resistance was assessed in the field as well as by detached leaf tests in the laboratory. Genotyping-by-sequencing was conducted to establish comprehensive genetic fingerprints for subsequent genetic mapping experiments. All three mapping approaches located the gene to the distal part of the long arm of chromosome 5D in the hexaploid oat genome sequences of OT3098 and ‘Sang.’ Markers from this region were homologous to a region of chromosome 2Ce of the C-genome species, Avena eriantha, the donor of Pm7, which appears to be the ancestral source of a translocated region on the hexaploid chromosome 5D.

Genetic mapping of the powdery mildew resistance gene Pm13 on oat chromosome 1D

“…In addition to Pm13, other PM resistance genes were also present in the FO-panel, as suggested by the identification of additional significant MTAs on chromosomes 1A, 5D, 6C, 7A and 7D. The MTAs on chromosome 1A, 5D and 7A can be attributed to the resistance genes Pm3 (Mohler, 2022), Pm7 (Brodführer et al, 2023) and Pm11 (Ociepa et al, 2020), respectively. To the best of our knowledge, the MTAs on 6C and 7D could be linked to new, unmapped resistance loci.…”

“…Molecular marker data of the 250 oat lines in the FO panel was extracted from Brodführer et al, (2023). The Genomic Association and Prediction Integrated Tool (GAPIT3) package in R (Version 4.1.0) was used for GWAS analysis (Wang et al, 2021).…”

“…For GWAS, a facultative oat panel (FO-panel) with 250 lines of similar panicle emergence and plant height of the larger KLAR panel described in Brodführer et al (2023) was used. For QTL mapping, a HuskyxAVE1284 bi-parental population was developed.…”

“…These varieties have been used as parents in several crossing programmes, resulting in varieties such as Flämingstip and Husky. A phylogenetic tree based on 8417 GBS markers (Brodführer et al, 2023) reflects the relationship between Husky, Flämingstip, Firth, Winston and some breeding lines (Supplementary Figure S1). Regarding the APR in Firth, a first QTL mapping approach was reported by Hagmann et al (2012), who described a major QTL near the DArT marker oPt-6125, which was mapped to linkage group 5_30 on the Kanota/Ogle map (KxO, Tinker et al, 2009).…”

Effects of Freeze–Thaw Cycles on the Quality of a Novel Mixed Grain Composite Dough and Its Product (Potato-Oat Yu): Hybridization of Potatoes and Oats

“…Besides being grown for grain production, oats are also commonly used as a forage crop for cereal and livestock farming. They are highly valued for their nutritional content, which benefits both human and animal diets . Both potatoes and oats are significant crops, with widespread cultivation and utilization worldwide.…”