Neuroprotective, antiapoptotic and antioxidant effects of<scp>l</scp>-carnitine against caffeine-induced neurotoxicity in SH-SY5Y neuroblastoma cell line

Bavari, Marzieh; Tabandeh, Mohammad Reza; Varzi, Hossein Najafzadeh; Bahramzadeh, Somayeh

doi:10.3109/01480545.2015.1063062

Cited by 25 publications

(15 citation statements)

References 32 publications

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“…The administration of acetyl-l-carnitine (500 µM) exhibited a neuroprotective role by restoring synaptic plasticity and transmission [65]. In a study by Bavari et al [66], the neuroprotective effect of l-carnitine (5 mM) controlled, within 18 h, caffeine cytotoxicity through the regulation of apoptosis-related caspase-3 activity, reducing the DNA fragmentation, inhibition of reactive oxygen species (ROS) generation, elevation of endogenous anti-oxidant defense systems, and the prevention of lipid oxidation. In vitro accumulated l-carnitine has been used to control DNA damage and oxidative damage in patients with mitochondrial fatty acid oxidation disorders [67].…”

Section: Activity Effect Referencesmentioning

confidence: 99%

The Nutraceutical Value of Carnitine and Its Use in Dietary Supplements

Durazzo¹,

Lucarini²,

Nazhand

et al. 2020

Molecules

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Carnitine can be considered a conditionally essential nutrient for its importance in human physiology. This paper provides an updated picture of the main features of carnitine outlining its interest and possible use. Particular attention has been addressed to its beneficial properties, exploiting carnitine’s properties and possible use by considering the main in vitro, in animal, and human studies. Moreover, the main aspects of carnitine-based dietary supplements have been indicated and defined with reference to their possible beneficial health properties.

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Section: Activity Effect Referencesmentioning

confidence: 99%

The Nutraceutical Value of Carnitine and Its Use in Dietary Supplements

Durazzo¹,

Lucarini²,

Nazhand

et al. 2020

Molecules

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“…SH-SY5Y neuroblastoma cells (human, ECACC; Sigma Aldrich, St. Louis, MO, USA) were used in this study. Selected cell line is frequently used as a reliable model for studying neurotoxicity of drugs [48,49]. SH-SY5Y cells were incubated at a density of 4 × 10 6 cells/well in 6-well culture plates in Ham's F-12 Nutrient Mixture (Thermo Fisher, Waltham, MA, USA) and minimum essential medium (MEM) (Sigma Aldrich, St. Louis, MO, USA) (mixed in ratio 1:1) medium supplemented with penicillin (100 U/mL), streptomycin (100 µg/mL), and L-glutamine (2 mM) without fetal bovine serum at 37 • C in saturated humidity atmosphere containing 5% CO 2 .…”

Section: Cell Culture and Incubationmentioning

confidence: 99%

Molecular Mechanisms of Bortezomib Action: Novel Evidence for the miRNA–mRNA Interaction Involvement

Łuczkowska

Rogińska

Ulańczyk

et al. 2020

IJMS

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Bortezomib is an anti-tumor agent, which inhibits 26S proteasome degrading ubiquitinated proteins. While apoptotic transcription-associated activation in response to bortezomib has been suggested, mechanisms related to its influence on post-transcriptional gene silencing mediated regulation by non-coding RNAs remain not fully elucidated. In the present study, we examined changes in global gene and miRNA expression and analyzed the identified miRNA–mRNA interactions after bortezomib exposure in human neuroblastoma cells to define pathways affected by this agent in this type of cells. Cell viability assays were performed to assess cytotoxicity of bortezomib. Global gene and miRNA expression profiles of neuroblastoma cells after 24-h incubation with bortezomib were determined using genome-wide RNA and miRNA microarray technology. Obtained results were then confirmed by qRT-PCR and Western blot. Further bioinformatical analysis was performed to identify affected biological processes and pathways. In total, 719 genes and 28 miRNAs were downregulated, and 319 genes and 61 miRNAs were upregulated in neuroblastoma cells treated with bortezomib. Possible interactions between dysregulated miRNA/mRNA, which could be linked to bortezomib-induced neurotoxicity, affect neurogenesis, cellular calcium transport, and neuron death. Bortezomib might exert toxic effects on neuroblastoma cells and regulate miRNA–mRNA interactions influencing vital cellular functions. Further studies on the role of specific miRNA–mRNA interactions are needed to elucidate mechanisms of bortezomib action.

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“…In the normal control group, cells were cultured in DMEM medium with only dimethyl sulphoxide (DMSO) added. In the caffeine treatment group, a toxic concentration of 5 mmol/L caffeine (Shenzhen phystandard Biotech Co., Ltd., China) was added to the medium [26]. In the lianzixin pretreatment groups, 5 mmol/L caffeine was added after the cells were pretreated with extracts of lianzixin provided by our research group as neferine, total alkaloids of lianzixin, alcohol extract of lianzixin and water extract of lianzixin for 6 h. Dose selection was based on a previous study [27].…”

Section: Experimental Designmentioning

confidence: 99%

Neferine and lianzixin extracts have protective effects on undifferentiated caffeine-damaged PC12 cells

Chen

Tang

Liu

et al. 2020

BMC Complement Med Ther

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Background: The embryos of Nelumbo nucifera Gaertn seeds, lianzixin, are used in China as food and traditional herbal medicine. Principal therapeutic indications are insomnia, anxiety and pyrexia. Caffeine is a psychostimulant and excessive use predisposes to cell damage and neurotoxicity. We aimed to investigate the potential protect effect of Neferine and lianzixin extracts on undifferentiated caffeine-damaged phaeochromocytoma cells (PC12 cells). Methods: A cell damage model based on undifferentiated PC12 was established with caffeine. Effect of Lianzixin extracts (total alkaloids, alcohol extract and water extract) and neferine on caffeine-damaged PC12 cells was evaluated. Cell viability was assessed using the methyl thiazolyl tetrazolium (MTT) assay, cellular morphology by inverted microscope, the nucleus by Hoechst 33342 staining and cleaved poly ADP-ribose polymerase (PARP) expression by western blot analysis. Results: Lianzixin extracts (total alkaloids, alcohol extract and water extract) and neferine improved the viability of PC12 cells damaged by caffeine. The morphology of PC12 cells pretreated with neferine, or alcohol or water extract of lianzixin aggregated and attached better than caffeine-damaged cells, but cells pretreated with total alkaloids of lianzixin showed abnormal morphology. Compared with caffeine-damaged cells, cells pretreated with neferine, or alcohol or water extract of lianzixin showed a notable increase in nucleus staining and an obvious decrease in cleaved PARP expression. Conclusions: Lianzixin extracts and neferine have protective effects against caffeine-induced damage in PC12 cells, which laid a foundation for finding a new medicine value of Lianzixin.

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Neuroprotective, antiapoptotic and antioxidant effects ofl-carnitine against caffeine-induced neurotoxicity in SH-SY5Y neuroblastoma cell line

Cited by 25 publications

References 32 publications

The Nutraceutical Value of Carnitine and Its Use in Dietary Supplements

The Nutraceutical Value of Carnitine and Its Use in Dietary Supplements

Molecular Mechanisms of Bortezomib Action: Novel Evidence for the miRNA–mRNA Interaction Involvement

Neferine and lianzixin extracts have protective effects on undifferentiated caffeine-damaged PC12 cells

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