Natural occurrence of scirpentriol in cereals infected by<i>Fusarium</i>species

Perkowski, J.; Kiecana, I.; Stachowiak, J; Basiński, Tomasz

doi:10.1080/0265203031000100773

Cited by 13 publications

(6 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Samples containing the highest concentration of DON were also contaminated with STO. Probably spikes/plots infected by DON producers were also infected by STO producing species ( Perkowski et al, 2003 ; Schollenberger et al, 2007 ).…”

Section: Resultsmentioning

confidence: 99%

Relationships between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars

Góral

Stuper‐Szablewska

Buśko

et al. 2015

The Plant Pathology Journal

View full text Add to dashboard Cite

Fusarium head blight is one of the most important and most common diseases of winter wheat. In order to better understanding this disease and to assess the correlations between different factors, 30 cultivars of this cereal were evaluated in a two-year period. Fusarium head blight resistance was evaluated and the concentration of trichothecene mycotoxins was analysed. Grain samples originated from plants inoculated with Fusarium culmorum and naturally infected with Fusarium species. The genetic distance between the tested cultivars was determined and data were analysed using multivariate data analysis methods. Genetic dissimilarity of wheat cultivars ranged between 0.06 and 0.78. They were grouped into three distinct groups after cluster analysis of genetic distance. Wheat cultivars differed in resistance to spike and kernel infection and in resistance to spread of Fusarium within a spike (type II). Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present. In control samples trichothecenes of groups A (H-2 toxin, T-2 toxin, T-2 tetraol, T-2 triol, scirpentriol, diacetoxyscirpenol) and B were detected. On the basis of Fusarium head blight assessment and analysis of trichothecene concentration in the grain relationships between morphological characters, Fusarium head blight resistance and mycotoxins in grain of wheat cultivars were examined. The results were used to create of matrices of distance between cultivars – for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test. A highly significant correlation between genetic distance and mycotoxin distance was found for the samples inoculated with Fusarium culmorum. Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.

show abstract

Section: Resultsmentioning

confidence: 99%

Relationships between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars

Góral

Stuper‐Szablewska

Buśko

et al. 2015

The Plant Pathology Journal

View full text Add to dashboard Cite

show abstract

“…They invade cereal crops and are responsible for the production toxic metabolites such as type A trichothecenes: T-2 toxin, HT-2 toxin, T-2 tetraol, T-2 triol, scirpentriol (STO), and diacetoxyscirpenol which may contaminate animal and human food. [ 4 5 6 7 8 9 10 11 12 13 ] Trichothecene is sesquiterpenoid highly toxic; T-2 toxin and HT-2 toxin are potent inhibitor of protein synthesis and are highly cytotoxic to eukaryotes. [ 14 ] T-2 toxin, HT-2 toxin found to be contaminants in wheat, maize, barley, oats, rice, beans, and soybeans as well as in some cereal-based products and have played a role in plant diseases.…”

Section: Introductionmentioning

confidence: 99%

Growth inhibition and morphological alteration of Fusarium sporotrichioides by Mentha piperita essential oil

et al. 2017

View full text Add to dashboard Cite

Objective:The aim of this study is to determine the phytochemical composition, antifungal activity of Mentha piperita essential oil (MPE) against Fusarium sporotrichioides.Methods:The phytochemical composition was conducted by gas chromatography mass spectrometry (GC MS) analysis and mycelia growth inhibition was determined by minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), the morphological characterization was observed by scanning electron microscopy. Finally, the membrane permeability was determined by the release of extracellular constituents, pH, and total lipid content.Result:In GC MS analysis, 22 metabolites were identified such as menthol, l menthone, pulegone, piperitone, caryophyllene, menthol acetate, etc. The antifungal activity against targeted pathogen, with MIC and MFC 500 μg/mL and 1000 μg/mL, respectively. The MPE altered the morphology of F. sporotrichoides hyphae with the loss of cytoplasm content and contorted the mycelia. The increasing concentration of MPE showed increase in membrane permeability of F. sporotrichoides as evidenced by the release of extracellular constituents and pH with the disruption of cell membrane indicating decrease in lipid content of F. sporotrichoides.Conclusion:The observed results showed that MPE exhibited promising new antifungal agent against Fusarium sporotrichioides.SUMMARY F. sporotrichioides, filamentous fungi contaminate to corn and corn--based productsF. sporotrichioides mainly responsible for the production of T-2 toxinPhytochemical composition was conducted by gas chromatography--mass spectrometry analysisMentha piperita essential oil (MPE) is commonly known as peppermintThe F. sporotrichioides growth was inhibited by MPE (minimum inhibitory concentration, minimum fungicidal concentration)Morphological observation by scanning electron microscope. Abbreviations Used: Cfu: Colony forming unit; DMSO: Dimethyl sulfoxide, °C: Degree celsius; F. Sporotrichoides: Fusarium sporotrichioides; EOs: Essential oils; M: Molar, g: Gram/gravity, mg: Milligram; μg: Microgram, ml: Milliliter; mm: Millimeter, min: Minutes; M. piperita: Mentha piperita, MIC: Minimum inhibitory concentration; MFC: Minimum fungicidal concentration; MAE: Mentha arvensis essential oil; Na2SO4: Sodium sulfate; pH: Potential Hydrogen; PDB: Potato Dextrose Broth; SEM: Scanning electron microscope

show abstract

“…This is probably caused by the fact that the highest amount of type A trichothecenes is accumulated in chaff. Perkowski et al (2003) reported that trichothecene levels in oats were the highest in chaff, lower in the apical internode of the stalk, and the lowest in grain. In case of isolate ZRF 41, the opposite situation was observed, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…sporotrichioides, like F. acuminatum, F. sambucinum and F. poae, produce type A trichothecenes: T-2 toxin, HT-2 toxin, T-2 tetraol, T-2 triol, scirpentriol (STO), and diacetoxyscirpenol (DAS). Those mycotoxins are accumulated in infected grain (Brown et al 2001;Mateo et al 2002;Moss 2002;Moss and Thrane 2004;Niesen et al 2004;Perkowski and Basiński 2002;Perkowski et al 2003;Perkowski et al 2007;Thrane et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Fusarium Sporotrichioides Sherb. Toxins Evaluated in Cereal Grain with Trichoderma Harzianum

Wiśniewska¹,

Basiński²,

Chełkowski³

et al. 2011

Journal of Plant Protection Research

View full text Add to dashboard Cite

Fusarium head blight is one of the most severe diseases of small grain cereals and is caused by several toxigenic Fusarium species. Yield losses and mycotoxin accumulation in grain are caused by the disease. F. sporotrichioides and F. poae produce type A trichothecenes. Saprophyte fungal antagonists, especially Trichoderma harzianum, are effective biocontrol agents against several fungal soil-borne plant pathogens. These fungal antagonists can reduce the production of Fusarium spp. mycotoxins in some crop plants. The aim of this study was to examine the influence of T. harzianum AN4 on the production of type A trichothecenes by F. sporotrichioides in cereals. The accumulation of six trichothecene mycotoxins (scirpentriol, i.e. STO, T-2 tetraol, T-2 triol, HT-2 toxin i.e., HT-2, T-2 toxin i.e., T-2, diacetoxyscirpenol, i.e., DAS) was reduced on average, by over 89% in bioassays of F. sporotrichioides and Trichoderma isolate AN4 on a liquid medium and on solid substrates (seeds of naked and husked oats and wheat). The reduction in our experiments depended on fungal isolate and substrate. From the three isolates of F. sporotrichioides used in the experiments, the highest accumulation of all the metabolites after 21 days by F. sporotrichioides in nearly all substrates, was recorded for strain ZFR 159. On the liquid medium inoculated with F. sporotrichioides ZFR 159, the amount of type A trichothecenes was the lowest (STO, T-2 tetraol, and T-2 triol not detected, HT-2 toxin 0.02 ppm, DAS 0.10 ppm, T-2 toxin 0.99 ppm). The highest total concentration of these toxins was produced by this isolate in husked oat cv. German (180.16 ppm), but in naked oat cv. Akt the toxin concentration was low (27.62 ppm). Trichothecene accumulation by T. harzianum AN4 was reduced the most in oat cv. Akt (98.48%) in the liquid medium (98.22%), while the lowest reduction was in oat cv. German (48.77%). The non-toxigenic T. harzianum AN4 isolate proved to be a useful biocontrol agent against the toxigenic F. sporotrichioides in cereals, significantly reducing the production of six type A trichothecenes. This is the first report on effective biocontrol of F. sporotrichioides in cereals by T. harzianum.

show abstract

Natural occurrence of scirpentriol in cereals infected byFusariumspecies

Cited by 13 publications

References 24 publications

Relationships between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars

Relationships between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars

Growth inhibition and morphological alteration of Fusarium sporotrichioides by Mentha piperita essential oil

Fusarium Sporotrichioides Sherb. Toxins Evaluated in Cereal Grain with Trichoderma Harzianum

Contact Info

Product

Resources

About