Natural attenuation of dengue virus type-2 after a series of island outbreaks: A retrospective phylogenetic study of events in the South Pacific three decades ago

Steel, Argon; Gubler, Duane J.; Bennett, Shannon N.

doi:10.1016/j.virol.2010.05.033

Cited by 74 publications

(70 citation statements)

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“…A larger sample size would be required to detect differences between these groups, if they exist. Furthermore, with the accumulating evidence that the strain of virus also influences clinical severity, [7][8][9] these types of studies must include different virus strains to be meaningful.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, given that host, 6 viral, and possibly vector-related factors [7][8][9] influence disease outcome, cross-sectional studies that compare primary and secondary infections may be confounded by many factors other than the serologic status of dengue at the point of infection. Cohort studies could address this question, although such studies are complex and resource intensive.…”

Section: Introductionmentioning

confidence: 99%

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Research on Dengue During World War II Revisited

Snow¹,

Haaland²,

Ooi³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. Much of the basic clinical information about dengue infection comes from experimental human studies conducted in the 1920s and 1940s. Albert Sabin's original laboratory records from one such study were bequeathed to Duane J. Gubler. These records were reviewed and 150 experiments were included in our analyses. Persons were inoculated with dengue virus 1 (DENV-1) and DENV-2. Median fever duration was shorter in primary DENV-2 infections compared with DENV-1, although maximum temperature and severity of illness were comparable. At 1.5-9 months after primary infection, 20 persons were inoculated with the heterologous serotype. Only one person inoculated with a heterologous serotype at 8 weeks showed development of a clinical infection with a maximum temperature of 38 C, and 7 (88%) of 8 persons inoculated with a heterologous serotype at 4-9 months post-primary infection showed development of fever. On average, persons had a shorter incubation period in secondary infection compared with primary infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research on Dengue During World War II Revisited

Snow¹,

Haaland²,

Ooi³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

Self Cite

View full text Add to dashboard Cite

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“…[25][26][27] Additionally, different genotypes within a given serotype and different lineages within a given genotype have been shown to vary in tendency to cause severe disease. [28][29][30] For example, the DENV-2 viruses circulating in the Americas were of the American genotype until 1981, when DENV-2 viruses of the southeast Asian/American genotype were detected in the region. 26 Infection with DENV-2 of the American genotype had limited association with severe disease in the Americas, although recent analyses indicate that outbreaks of this American genotype in the 1970s in Puerto Rico, Tahiti, New Caledonia, and Niue included severe dengue disease.…”

Section: Introductionmentioning

confidence: 99%

“…26 Infection with DENV-2 of the American genotype had limited association with severe disease in the Americas, although recent analyses indicate that outbreaks of this American genotype in the 1970s in Puerto Rico, Tahiti, New Caledonia, and Niue included severe dengue disease. [30][31][32][33][34] Nonetheless, the invasion of the southeast Asian/ American genotype DENV-2 resulted in the first major DHF epidemic in the Americas. 35 Experimental studies revealed that the American genotype DENV-2 had lower replication capacity in cultured human cells and reduced infectivity for Ae.…”

Section: Introductionmentioning

confidence: 99%

Lineage II of Southeast Asian/American DENV-2 is Associated with a Severe Dengue Outbreak in the Peruvian Amazon

Williams¹,

Mayer²,

Johnson³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.

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“…In the host, immune status, age, sex, and genetic factors may contribute to disease severity. Numerous field studies have indicated a link between disease severity and the DENV genome sequence (2,3,22,27,38). A number of viral determinants modulating the intrinsic virulence of the virus within its host have been identified and have been shown to affect either the binding properties (7,11,33,37) or the replication efficiency (20, 34) of DENV.…”

mentioning

confidence: 99%

A Single Amino Acid in Nonstructural Protein NS4B Confers Virulence to Dengue Virus in AG129 Mice through Enhancement of Viral RNA Synthesis

Grant

Tan

Qing

et al. 2011

J Virol

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Dengue (DEN) is a mosquito-borne viral disease that has become an increasing economic and health burden for the tropical and subtropical world. The lack of an appropriate animal model of DEN has greatly impeded the study of its pathogenesis and the development of vaccines/antivirals. We recently reported a DEN virus 2 (DENV-2) strain (D2Y98P) that lethally infects immunocompromised AG129 mice, resulting in organ damage or dysfunction and increased vascular permeability, hallmarks of severe DEN in patients (G. K. Tan et al., PLoS Negl. Trop. Dis. 4:e672, 2010). Here we report the identification of one critical virulence determinant of strain D2Y98P. By mutagenesis, we showed that a Phe-to-Leu alteration at amino acid position 52 in nonstructural protein NS4B completely abolished the pathogenicity of the D2Y98P virus, as evidenced by a lack of lethality and the absence of histological signs of disease, which correlated with reduced viral titers and intact vascular permeability. Conversely, a Leu-to-Phe alteration at position 52 of NS4B in nonvirulent DENV-2 strain TSV01 led to 80% lethality and increased viremia. The NS4B(Phe52) viruses displayed enhanced RNA synthesis in mammalian cells but not in mosquito cells. The increased viral RNA synthesis was independent of the ability of NS4B to interfere with the host type I interferon response. Overall, our results demonstrate that Phe at position 52 in NS4B confers virulence in mice on two independent DENV-2 strains through enhancement of viral RNA synthesis. In addition to providing further insights into the functional role of NS4B protein, our findings further support a direct relationship between viral loads and DEN pathogenesis in vivo, consistent with observations in DEN patients.Dengue (DEN) disease, caused by any one of the four serotypes of dengue virus (DENV), is a major global public health and economic burden. DENV affects 2.5 billion people worldwide, mostly in tropical and subtropical countries, leading to 50 to 100 million human infections each year (13). Clinical manifestations of DEN disease range from undifferentiated fever to DEN fever (DF) and DEN hemorrhagic fever (DHF), with plasma leakage that may lead to hypovolemic shock (DEN shock syndrome [DSS]). Therapeutic intervention is limited to fluid management for DEN patients who experience severe vascular leakage (41). Currently, no antiviral therapy or vaccine to fight DEN is available on the market (9, 31).DENV belongs to the genus Flavivirus within the family Flaviviridae. Besides DENV, many other flaviviruses are important human pathogens, including yellow fever virus (YFV), West Nile virus (WNV), Japanese encephalitis virus (JEV), and tick-borne encephalitis virus (TBEV). DENV is a small enveloped virus with a positive single-strand RNA of approximately 10,700 nucleotides (18). The DENV genome encodes 10 proteins, comprising 3 structural proteins (capsid [C], premembrane [PrM], and envelope [E]) and 7 nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The structural proteins fo...

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Natural attenuation of dengue virus type-2 after a series of island outbreaks: A retrospective phylogenetic study of events in the South Pacific three decades ago

Cited by 74 publications

References 40 publications

Research on Dengue During World War II Revisited

Research on Dengue During World War II Revisited

Lineage II of Southeast Asian/American DENV-2 is Associated with a Severe Dengue Outbreak in the Peruvian Amazon

A Single Amino Acid in Nonstructural Protein NS4B Confers Virulence to Dengue Virus in AG129 Mice through Enhancement of Viral RNA Synthesis

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