Lineage II of Southeast Asian/American DENV-2 is Associated with a Severe Dengue Outbreak in the Peruvian Amazon

Williams, M. C.; Mayer, Sandra V.; Johnson, William L.; Chen, Rubing; Volkova, Evgeniya; Vilcarromero, Stalin; Widen, Steven G.; Wood, Thomas G.; Suárez-Ognio, Luis; Long, Kanya C.; Hanley, Kathryn A.; Morrison, Amy C.; Vasilakis, Nikos; Halsey, Eric S.

doi:10.4269/ajtmh.13-0600

Cited by 60 publications

(62 citation statements)

References 55 publications

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“…Our study reports that the Panamanian DENV-2 strain, circulating during 2011 outbreak, belongs to Asian/American genotype (AS/AM). The introduction of this genotype has been linked with outbreaks with high mortality rates 41,45,46 in Peru 47 , Nicaragua 27 and Brasil 48 . Our phylogenetic analysis clustered Panama 2011 DENV-2 strains in clade 2 sub-clade 2b.I using previous classification of DENV-2 AS/AM circulating in Central America 26 , closely related with strains from Mexico 2011 and Guatemala 2009.…”

Section: Discussionmentioning

confidence: 99%

The reintroduction of DENV-2 in 2011 in Panama and subsequent outbreak characteristic

et al. 2018

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The circulation of the South-east Asian/American (AS/AM) dengue 2 virus (DENV-2) genotype in the Americas has been associated with a high rate of severe disease. From 1993, the year DENV was reintroduced in Panama, until 2011 there were 29 dengue-associated deaths, 17 of which occurred in 2011, the most severe outbreak with a case fatality rate (CFR) of 44% (17 deaths out of 38 severe dengue cases). During this outbreak DENV-2 was reintroduced into the country, whereas over the prior five years DENV-1 and −3 were predominant. Herein, we describe the 2011 Panama outbreak and genetically characterize the Panamanian DENV-2 strains, which were associated with severe dengue disease in Panama. Our results suggest that the DENV-2 isolates from this outbreak belonged to the AS/AM genotype sub-clade 2BI and were genetically close to viruses described in the outbreaks in Nicaragua, Honduras, Guatemala and Mexico from 2006–2011. Sub-clade 2BI has previously been associated with severe disease in Nicaragua during outbreaks from 2005–2007.

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Section: Discussionmentioning

confidence: 99%

The reintroduction of DENV-2 in 2011 in Panama and subsequent outbreak characteristic

et al. 2018

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“…FFAs were conducted as described previously ( 23 ) for viral stocks, blood meals, and all mosquito samples by inoculating 96-well plates of nearly confluent Vero cells with 50 μL of sample supplemented with 50 μL of mosquito media. After a 3-day incubation, plates were fixed, washed, and blocked before overnight incubation with mouse anti– Zika virus (strain MR-766).…”

Section: Methodsmentioning

confidence: 99%

Variation inAedes aegyptiMosquito Competence for Zika Virus Transmission

Roundy¹,

Azar²,

Rossi³

et al. 2017

Emerg. Infect. Dis.

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To test whether Zika virus has adapted for more efficient transmission by Aedes aegypti mosquitoes, leading to recent urban outbreaks, we fed mosquitoes from Brazil, the Dominican Republic, and the United States artificial blood meals containing 1 of 3 Zika virus strains (Senegal, Cambodia, Mexico) and monitored infection, dissemination, and virus in saliva. Contrary to our hypothesis, Cambodia and Mexica strains were less infectious than the Senegal strain. Only mosquitoes from the Dominican Republic transmitted the Cambodia and Mexica strains. However, blood meals from viremic mice were more infectious than artificial blood meals of comparable doses; the Cambodia strain was not transmitted by mosquitoes from Brazil after artificial blood meals, whereas 61% transmission occurred after a murine blood meal (saliva titers up to 4 log10 infectious units/collection). Although regional origins of vector populations and virus strain influence transmission efficiency, Ae. aegypti mosquitoes appear to be competent vectors of Zika virus in several regions of the Americas.

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“…In Peru, a dengue outbreak during 2010 and 2011 was unprecedented in its magnitude and severity. Whole-genome sequencing documented that the responsible virus was a DENV-2 belonging to lineage II of Southeast Asian/American genotype that differed from the DENV-2 virus that had been circulating in the region since 1990 [9]. In this case, it appeared that the introduction of a new virus lineage contributed to the changed epidemiology.…”

Section: Americasmentioning

confidence: 95%

“…These techniques have identified clades and genotypes within a serotype and determined genetic relatedness of the viruses [5,7]. Based on these phylogenetic analyses, further understanding of dengue virus origin, movement, and distribution has become possible [6,8,9].…”

Section: Mapping and Estimating Disease Burden Of Denguementioning

confidence: 99%

Dengue: Update on Epidemiology

Wilson

Chen

2014

Curr Infect Dis Rep

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The epidemiology of dengue fever has undergone major shifts in recent decades. The global distribution has expanded to include more geographic areas. The intensity of transmission and the severity of infections have increased in areas where infection was already endemic. Multiple studies provide a clearer picture of the epidemiology and allow mapping of its distribution and change over time. Despite major efforts to control transmission, competent vectors now infest most tropical and subtropical regions; Aedes albopictus, also a competent vector, is able to survive in temperate areas, placing parts of Europe and North America at risk for local transmission. Many research teams in dengue-endemic areas are working to identify key local weather, vector, and other variables that would allow prediction of a likely epidemic early enough to permit interventions to avert it or blunt its impact.

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Lineage II of Southeast Asian/American DENV-2 is Associated with a Severe Dengue Outbreak in the Peruvian Amazon

Cited by 60 publications

References 55 publications

The reintroduction of DENV-2 in 2011 in Panama and subsequent outbreak characteristic

The reintroduction of DENV-2 in 2011 in Panama and subsequent outbreak characteristic

Variation inAedes aegyptiMosquito Competence for Zika Virus Transmission

Dengue: Update on Epidemiology

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