Research on Dengue During World War II Revisited

Snow, Grace E; Haaland, Benjamin; Ooi, Eng Eong; Gubler, Duane J.

doi:10.4269/ajtmh.14-0132

Cited by 67 publications

(50 citation statements)

References 47 publications

(61 reference statements)

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“…In order to maximize our chance of observing any potential effect of T cells, we chose to challenge with virus soon after the peak, before the T cell levels were back to prepriming levels. Also, challenging 2 weeks after priming ensured that we were challenging within the shortest duration that has been postulated for the cross-protection period observed after DENV infection (14,17).…”

Section: Resultsmentioning

confidence: 99%

“…The duration of the cross-protection period remains a matter of debate (13)(14)(15)17). A recent reanalysis of historical data suggests a duration of 8 weeks (17). Based on serology and on epidemic modeling, other estimates vary from 1 to 2 weeks to 1 year or more (13)(14)(15).…”

mentioning

confidence: 99%

“…Based on serology and on epidemic modeling, other estimates vary from 1 to 2 weeks to 1 year or more (13)(14)(15). In addition to the duration, the mechanism of cross-protection remains elusive too (17). The transient cross-protection is often assumed to rely on high titers of cross-reactive antibodies reactive for all DENV serotypes (18)(19)(20), but experimental evidence is scarce.…”

mentioning

confidence: 99%

“…Additionally, after infection, there is a period of protection against heterotypic infection with other serotypes (cross-protection) (13)(14)(15)(16)(17). The duration of the cross-protection period remains a matter of debate (13)(14)(15)17).…”

mentioning

confidence: 99%

“…In order to maximize the chance of capturing the potential contribution of T cells, we chose to challenge with virus shortly after the peak of the T cell response (expected around days 7 to 10), but before T cell responses return to the prepriming level (28). This approach also allowed us to be within the 2-week period that has been postulated as being the shortest period of cross-protection according to the most conservative estimates (14,17).…”

mentioning

confidence: 99%

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CD8 ⁺ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice

Zellweger

Tang

Eddy

et al. 2015

J Virol

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Dengue virus (DENV) is a major public health threat worldwide. Infection with one of the four serotypes of DENV results in a transient period of protection against reinfection with all serotypes (cross-protection), followed by lifelong immunity to the infecting serotype. While a protective role for neutralizing antibody responses is well established, the contribution of T cells to reinfection is less clear, especially during heterotypic reinfection. This study investigates the role of T cells during homotypic and heterotypic DENV reinfection. Mice were sequentially infected with homotypic or heterotypic DENV serotypes, and T cell subsets were depleted before the second infection to assess the role of DENV-primed T cells during reinfection. Mice primed nonlethally with DENV were protected against reinfection with either a homotypic or heterotypic serotype 2 weeks later. Homotypic priming induced a robust neutralizing antibody response, whereas heterotypic priming elicited binding, but nonneutralizing antibodies. CD8 ؉ T cells were required for protection against heterotypic, but not homotypic, reinfection. These results suggest that T cells can contribute crucially to protection against heterotypic reinfection in situations where humoral responses alone may not be protective. Our findings have important implications for vaccine design, as they suggest that inducing both humoral and cellular responses during vaccination may maximize protective efficacy across all DENV serotypes. IMPORTANCEDengue virus is present in more than 120 countries in tropical and subtropical regions. Infection with dengue virus can be asymptomatic, but it can also progress into the potentially lethal severe dengue disease. There are four closely related dengue virus serotypes. Infection with one serotype results in a transient period of resistance against all serotypes (cross-protection), followed by lifelong resistance to the infecting serotype, but not the other ones. The duration and mechanisms of the transient cross-protection period remain elusive. This study investigates the contribution of cellular immunity to cross-protection using mouse models of DENV infection. Our results demonstrate that cellular immunity is crucial to mediate cross-protection against reinfection with a different serotype, but not for protection against reinfection with the same serotype. A better understanding of the mediators responsible for the cross-protection period is important for vaccine design, as an ideal vaccine against dengue virus should efficiently protect against all serotypes.T he four serotypes of dengue virus (DENV) are the etiologic agent of dengue, a rapidly spreading arboviral disease that is present in more than 120 countries (1-5). Recent estimates suggest that more than 3.5 billion people living in tropical and subtropical regions are at risk of infection, with 390 million infections per year, of which 96 million are symptomatic (1-3).Infection with DENV is often asymptomatic (6, 7), but if disease is apparent, it ranges from den...

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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CD8 ⁺ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice

Zellweger

Tang

Eddy

et al. 2015

J Virol

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Evaluation of the oral immunogenicity of M cell-targeted tetravalent EDIII antigen for development of plant-based edible vaccine against dengue infection

Kim

2019

Plant Cell Tiss Organ Cult

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Four serotypes of the dengue virus that can cause severe disease in humans greatly increases the complexity of vaccine development. In this study, we evaluated an 'all-in-one' tetravalent dengue vaccine candidate as an alternative to the classical four viruses 'cocktail' vaccination approach. A single polypeptide chain of the tetra-EDIII comprising all four serotypes fused to the M-cell target ligand peptide (tEDIII-Co1) was constructed and expressed using a rice expression system under the control of a strong inducible promoter (rice amylase 3D: Ramy3D) and rice 3′ untranslated region (UTR). The tEDIII-Co1 was constructed using wild type or plant codon optimized gene sequence to compare the expression levels. The codon optimized gene combined with ER-localization showed higher and more stable expression than codon-optimization alone or the wild type. To test the oral immunogenicity of this construct, the transgenic rice cells containing 100 µg of tEDIII-Co1 were fed to mice and the antigen specific IgG and IgA antibodies analysed in sera or fecal extracts. The tEDIII fused to Co1 induced strong antibody responses in the absence of any adjuvants. We observed the strongest antigen-specific B and T cell responses in animals immunized with tEDIII-Co1. Taken together, this simple tetravalent EDIII-based fusion protein with the M cell ligand could has potential as an alternative dengue vaccine strategy against all four serotypes.

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Day-to-Day Population Movement and the Management of Dengue Epidemics

Falcón-Lezama

Martínez-Vega²,

Kuri‐Morales³

et al. 2016

Bull Math Biol

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Dengue is a growing public health problem in tropical and subtropical cities. It is transmitted by mosquitoes, and the main strategy for epidemic prevention and control is insecticide fumigation. Effective management is, however, proving elusive. People’s day-to-day movement about the city is believed to be an important factor in the epidemiological dynamics. We use a simple model to examine the fundamental roles of broad demographic and spatial structures in epidemic initiation, growth and control. We show that the key factors are local dilution, characterised by the vector–host ratio, and spatial connectivity, characterised by the extent of habitually variable movement patterns. Epidemic risk in the population is driven by the demographic groups that frequent the areas with the highest vector–host ratio, even if they only spend some of their time there. Synchronisation of epidemic trajectories in different demographic groups is governed by the vector–host ratios to which they are exposed and the strength of connectivity. Strategies for epidemic prevention and management may be made more effective if they take into account the fluctuating landscape of transmission intensity associated with spatial heterogeneity in the vector–host ratio and people’s day-to-day movement patterns.Electronic supplementary materialThe online version of this article (doi:10.1007/s11538-016-0209-6) contains supplementary material, which is available to authorized users.

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Research on Dengue During World War II Revisited

Cited by 67 publications

References 47 publications

CD8 ⁺ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice

CD8 ⁺ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice

Evaluation of the oral immunogenicity of M cell-targeted tetravalent EDIII antigen for development of plant-based edible vaccine against dengue infection

Day-to-Day Population Movement and the Management of Dengue Epidemics

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Research on Dengue During World War II Revisited

Cited by 67 publications

References 47 publications

CD8 + T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice

CD8 + T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice

Evaluation of the oral immunogenicity of M cell-targeted tetravalent EDIII antigen for development of plant-based edible vaccine against dengue infection

Day-to-Day Population Movement and the Management of Dengue Epidemics

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CD8 ⁺ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice

CD8 ⁺ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice