Nanozyme Impregnated Mesenchymal Stem Cells for Hepatic Ischemia-Reperfusion Injury Alleviation

Abstract

Mesenchymal stem cell (MSC) based therapy holds great potential for treating numerous diseases owing to their capability to heal injured tissue/organs through paracrine factors secretion and immunomodulation. Despite the high hopes, the low viability of transplanted cells in the injured tissues due to the elevated oxidative stress levels remains the largest obstacle in MSCbased cell therapy. To achieve desired therapeutic efficiency, the survival of the transplanted MSCs in the high oxidative stress environment needs to be ensured. Herein, we proposed the use of a ROS-scavenging nanozyme to protect transplanted MSCs from oxidative stress-mediated apoptosis and thereby improve the therapeutic effect. Prussian blue (PB) nanoparticles as a biocompatible ROS-scavenging nanozyme were incorporated into the MSCs without affecting the stemness and differentiation potential of MSCs. The nanozyme impregnation significantly improved the survival of MSCs in a high oxidative stress condition as well as augmented their paracrine effect and anti-inflammatory properties, resulting in a profound therapeutic effect in vivo in the liver ischemia-reperfusion (I/R) injury animal model. Our results indicated that the nanozyme incorporation into MSCs is a simple but efficient way to improve the therapeutic potential of MSC-based cell therapy.

Structure design mechanisms and inflammatory disease applications of nanozymes

“…and inhibiting their activation, to achieve further anti-inflammation effects. [27][28][29][30] Hence, utilizing nanozymes as a 'drug' for these inflammatory diseases is feasible and has great potential for clinical translation.…”

Harnessing reactive oxygen/nitrogen species and inflammation: Nanodrugs for liver injury

“…Recently, Tae et al. [ 306 ] combined MSCs with Prussian blue nanoparticles (PB) comprising a biocompatible nanozyme with multiple ROS-scavenging mimetic activity to protect against oxidative stress in liver with I/R injury ( Fig. 17 D).…”

Nanoplatform‐Based Reactive Oxygen Species Scavengers for Therapy of Ischemia‐Reperfusion Injury

“…Recently, Prussian Blue nanozyme‐impregnated mesenchymal stem cells were also developed for alleviation of hepatic IRI. [ 146 ] The nanozyme‐incorporated mesenchymal stem cells have significantly improved survival rate in a high oxidative stress condition as well as enhanced paracrine effects and anti‐inflammatory properties. They have profound therapeutic effect on liver IRI.…”