Nanoengineered myogenic scaffolds for skeletal muscle tissue engineering

Quint, Jacob; Samandari, Mohamadmahdi; Abbasi, Laleh; Lara, Evelyn Carolina Mollocana; Rinoldi, Chiara; Mostafavi, Azadeh; Tamayol, Ali

doi:10.1039/d1nr06143g

Cited by 31 publications

(32 citation statements)

References 50 publications

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“…Nanoclays are silicate-derived multilayers that have been shown to play a key role in the physiology of a wide range of tissues such as bone [48]. Several studies have proved that these nanoparticles are able to complement the already adequate properties of gelatin hydrogels for bone tissue regeneration [16,[49][50][51]. One of the main advantages of nanoclays consists in improving the gelatin's poor mechanical properties.…”

Section: Bioprintingmentioning

confidence: 99%

Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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Tissue engineering has become a medical alternative in this society with an ever-increasing lifespan. Advances in the areas of technology and biomaterials have facilitated the use of engineered constructs for medical issues. This review discusses on-going concerns and the latest developments in a widely employed biomaterial in the field of tissue engineering: gelatin. Emerging techniques including 3D bioprinting and gelatin functionalization have demonstrated better mimicking of native tissue by reinforcing gelatin-based systems, among others. This breakthrough facilitates, on the one hand, the manufacturing process when it comes to practicality and cost-effectiveness, which plays a key role in the transition towards clinical application. On the other hand, it can be concluded that gelatin could be considered as one of the promising biomaterials in future trends, in which the focus might be on the detection and diagnosis of diseases rather than treatment.

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Section: Bioprintingmentioning

confidence: 99%

Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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“…Calibration curve was designed at + 0.6 V from the concentration of acetylcholine and observed to be linear [R 2 = 0.7528, SD = 12.35, RSD = 49.05 %, error bar = 10.0 %, and n = 9] (Figure 8b). Analytical parameters of the prepared sensor [Sensitivity (101.27 nAμM À 1 cm À 2 ), low LOD to ∼ 11.58 pM and LOQ (38.60 pM)] were calculated using the equations (6)(7)(8). Here, m represents the slope of the calibration curve (y = 0.0032 x + 21.172), A represents the active surface area (0.0316 cm 2 ) of the provided electrodes and SD represents the standard deviation (12.35 μA) of acetylcholine concentration in the calibrated potential of + 0.6 V. [28] The linear dynamic range (100 pM ∼ 100 μM) and linearity (R 2 = 0.9520) following an equation, y = 2.1163x + 21.745, was also found from the calibration curve (Figure 8c).…”

Section: Acetylcholine Detection With Gce/v 2 O 5 Nrs/bpm Sensormentioning

confidence: 99%

“…[3][4][5] In the previous studies, it was reported that bio-molecules could be determined by using different experimental methods such as Capillary Electrophoresis, Chemiluminescence, Electrochemical Analysis, Fluorescence Analytical Procedure, High-Performance Liquid Chromatography, High Performance Liquid Chromatography Mass Spectrometry, Liquid Chromatography Tandem Mass Spectrometry, Paper Spray Mass Spectrometry, Spectro-photometry, Surface Plasmon Resonance, Thin Layer Chromatography and ultraviolet-visible spectro-photometry. [6][7][8][9][10][11] A sensor is an investigative instrument that combines an element with a physicochemical transducer to recognize a particular component. A transducer converts the emerging indication from the analyte's interaction with the element into a quantitative signal (voltage and current) that may be easily quantified.…”

Section: Introductionmentioning

confidence: 99%

“…Nerve illnesses, such as Alzheimer's, Parkinson's and Huntington's diseases, Myasthenia Gravis, motor dysfunction, Schizophrenia and progressive dementia, are linked to abnormal acetylcholine levels [3–5] . In the previous studies, it was reported that bio‐molecules could be determined by using different experimental methods such as Capillary Electrophoresis, Chemiluminescence, Electrochemical Analysis, Fluorescence Analytical Procedure, High‐Performance Liquid Chromatography, High Performance Liquid Chromatography Mass Spectrometry, Liquid Chromatography Tandem Mass Spectrometry, Paper Spray Mass Spectrometry, Spectro‐photometry, Surface Plasmon Resonance, Thin Layer Chromatography and ultraviolet‐visible spectro‐photometry [6–11] . A sensor is an investigative instrument that combines an element with a physicochemical transducer to recognize a particular component.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Acetylcholine in an Enzyme‐Free System Based on a GCE/V2O5 NRs/BPM Modified Sensor

et al. 2022

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In this approach, vanadium pentoxide nanorods (V 2 O 5 NRs) were prepared by utilizing a facile wet-chemical approach (WCA) in an alkaline phase at low temperatures then applied to detect acetylcholine. Various optical techniques, such as Ultra-Violet spectroscopy (UV-Visible), Fourier Transform Infra-red spectroscopy (FTIR), Powder X-ray Diffraction (XRD), Field Emission Scanning Emission Microscopy (FESEM), X-ray Electron Dispersive Spectroscopy (XEDS) and X-ray Photo-electron Spectroscopy (XPS) were applied to analyse the V 2 O 5 NRs for structural, optical, functional, morphological and elemental analyses. Adjustment of the light weight fabrication of NRs onto Glassy carbon electrode (GCE) exhibited sensitive and selective acetylcholine sensor probe. Based on the stable current-voltage relationship, analytical sensing parameters like sensitivity, Linear dynamic range (LDR), Limit of detection (LOD), Limit of quantification (LOQ), durability and interference measurement of the fabricated sensor (GCE/V 2 O 5 NRs/BPM) for acetylcholine detection were evaluated and reported in this paper. Here, Nafion was used as a binding polymer matrix (BPM) for the fabrication of flat GCE with V 2 O 5 NRs. Acetylcholine calibration curve was found to be linear throughout in a broad range of concentration of target acetylcholine analyte. Calibration curve was employed to calculate the sensing parameters such as sensitivity (101.27 nAμM À 1 cm À 2 ), LOD down to 11.58 pM, LOQ (38.60 pM) and LDR (100 pM ∼ 100 μM) of the developed GCE/V 2 O 5 NRs/BPM sensor probe. The use of the facile WCA method for the synthesis of low-dimensional V 2 O 5 NRs is a good strategy for developing an un-doped nanomaterial based sensor for enzyme-free bio-molecule recognition and detection via this electrochemical approach. The developed (GCE/V 2 O 5 NRs/BPM) sensor probe could be used to selectively detect acetylcholine in real biological samples by electrochemical method with satisfactory outcomes.

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“…We hence choose ADSCs as parent cells to produce exosomes, and investigate the effects of ADSCs exosomes on microtia chondrocytes as well as the underlying mechanism of action. With excellent mechanical properties, superior biocompatibility, antibacterial ability and bioactivity, Gelma hydrogel has been widely applied to the field of 3D culture [ 26 ] and controlled delivery of bioactive factors [ 27 ]. The porous Gelma with reticular pores size of 100 to 200 μm could be favorable to promote ADSCs adhesion/proliferation and nutrients transferring.…”

Section: Introductionmentioning

confidence: 99%

Congenital microtia patients: the genetically engineered exosomes released from porous gelatin methacryloyl hydrogel for downstream small RNA profiling, functional modulation of microtia chondrocytes and tissue-engineered ear cartilage regeneration

et al. 2022

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Background Mesenchymal stem cells (MSCs) exosomes were previously shown to be effective in articular cartilage repairing. However, whether MSCs exosomes promote mature cartilage formation of microtia chondrocytes and the underlying mechanism of action remains unknown. Additionally, some hurdles, such as the low yield and unsatisfactory therapeutic effects of natural exosomes have emerged when considering the translation of exosomes-therapeutics to clinical practices or industrial production. Herein, we investigated the roles of human adipose-derived stem cells (ADSCs) exosomes in modulating microtia chondrocytes and the underlying mechanism of action. Special attention was also paid to the mass production and functional modification of ADSCs exosomes. Results We firstly used porous gelatin methacryloyl (Porous Gelma) hydrogel with pores size of 100 to 200 μm for 3D culture of passage 2, 4 and 6 ADSCs (P2, P4 and P6 ADSCs, respectively), and obtained their corresponding exosomes (Exo 2, Exo 4 and Exo 6, respectively). In vitro results showed Exo 2 outperformed both Exo 4 and Exo 6 in enhancing cell proliferation and attenuating apoptosis. However, both Exo 4 and Exo 6 promoted chondrogenesis more than Exo 2 did. Small RNA sequencing results indicated Exo 4 was similar to Exo 6 in small RNA profiles and consistently upregulated PI3K/AKT/mTOR signaling pathway. Notably, we found hsa-miR-23a-3p was highly expressed in Exo 4 and Exo 6 compared to Exo 2, and they modulated microtia chondrocytes by transferring hsa-miR-23a-3p to suppress PTEN expression, and consequently to activate PI3K/AKT/mTOR signaling pathway. Then, we designed genetically engineered exosomes by directly transfecting agomir-23a-3p into parent P4 ADSCs and isolated hsa-miR-23a-3p-rich exosomes for optimizing favorable effects on cell viability and new cartilage formation. Subsequently, we applied the engineered exosomes to in vitro and in vivo tissue-engineered cartilage culture and consistently found that the engineered exosomes could enhance cell proliferation, attenuate apoptosis and promote cartilage regeneration. Conclusions Taken together, the porous Gelma hydrogel could be applied to exosomes mass production, and functional modification could be achieved by selecting P4 ADSCs as parent cells and genetically modifying ADSCs. Our engineered exosomes are a promising candidate for tissue-engineered ear cartilage regeneration. Graphical Abstract

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Nanoengineered myogenic scaffolds for skeletal muscle tissue engineering

Abstract: Here, we developed a nano-engineered biomaterial optimized for skeletal muscle proliferation and differentiation. Nanoclay disks were used to control the release of insulin-like growth factor (IGF-1), a key myogenic and immunogenic regulator.

Cited by 31 publications

References 50 publications

Progress in Gelatin as Biomaterial for Tissue Engineering

Progress in Gelatin as Biomaterial for Tissue Engineering

Detection of Acetylcholine in an Enzyme‐Free System Based on a GCE/V2O5 NRs/BPM Modified Sensor

Congenital microtia patients: the genetically engineered exosomes released from porous gelatin methacryloyl hydrogel for downstream small RNA profiling, functional modulation of microtia chondrocytes and tissue-engineered ear cartilage regeneration

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