Myofibroblasts from salivary gland adenoid cystic carcinomas promote cancer invasion by expressing <scp>MMP</scp>2 and <scp>CXCL</scp>12

Guan, Haijie; Tan, Jie; Zhang, Fuyin; Bai, Liang; Qi, Dongyuan; Dong, Hui; Zhu, Lei; Li, Xiaojie; Liu, Tingjiao

doi:10.1111/his.12519

Cited by 17 publications

(11 citation statements)

References 43 publications

(96 reference statements)

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“…Our previous study demonstrated that ACC-derived CAFs promoted ACC cell invasion in a 3D matrix in a spheroid fashion[ 21 ]. Furthermore, we demonstrated that ACC-derived CAFs showed high levels of MMP2 and CXCL12 expression, which might be related to the aggressive growth behavior of ACC[ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Carcinoma-Associated Fibroblasts Lead the Invasion of Salivary Gland Adenoid Cystic Carcinoma Cells by Creating an Invasive Track

Jia

Kong

et al. 2016

PLoS ONE

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Carcinoma-associated fibroblasts (CAFs) are critical in determining tumor invasion and metastasis. However the role of CAFs in the invasion of salivary gland adenoid cystic carcinoma (ACC) is poorly understood. In this study, we isolated primary CAFs from two ACC patients. ACC-derived CAFs expressed typical CAF biomarkers and showed increased migration and invasion activity. Conditioned medium collected from CAFs significantly promoted ACC cell migration and invasion. Co-culture of CAFs with ACC cells in a microfluidic device further revealed that CAFs localized at the invasion front and ACC cells followed the track behind the CAFs. Interfering of both matrix metalloproteinase and CXCL12/CXCR4 pathway inhibited ACC invasion promoted by CAFs. Overall, our study demonstrates that ACC-derived CAFs exhibit the most important defining feature of CAFs by promoting cancer invasion. In addition to secretion of soluble factors, CAFs also lead ACC invasion by creating an invasive track in the ECM.

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Section: Introductionmentioning

confidence: 99%

Carcinoma-Associated Fibroblasts Lead the Invasion of Salivary Gland Adenoid Cystic Carcinoma Cells by Creating an Invasive Track

Jia

Kong

et al. 2016

PLoS ONE

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“…Expression of MMP-2, MMP-9 and their tissue inhibitors in benign and malignant salivary gland tumors showed an imbalance between MMPs and their tissue inhibitors in malignant neoplasms and invasive properties related to MMP-2 [15]. Guan et al, [10] also evaluated MMP-2 expression in salivary gland adenoid cystic carcinoma. Their result emphasized on the role of high levels of MMP-2 expression in aggressive behavior of tumor.…”

Section: Discussionmentioning

confidence: 99%

“…Extracellular matrix (ECM) molecules are modified by matrix metalloproteinases (MMPs), a family of enzymes that can modulate cell density by creating space for migration, releasing ECMbound growth factors and activating signaling molecules. MMP-2 and MMP-9 are type IV collagenases that degrade the major structural protein of extracellular matrix and basement membrane [10]. The prognostic significance of MMP-2, 9 expressions has been reported in prostate cancer and OSCC [11,12].…”

Section: Introductionmentioning

confidence: 99%

Clinicopathologic Significance of P63, Maspin and MMP-2 Expression in Mucoepidermoid Carcinoma

Taghavi

Khozestani

Baghban

2017

J Islam Dent Assoc Iran

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Background and Aim: Mucoepidermoid carcinoma (MEC) is the most common malignant neoplasm of the salivary glands with complex histopathologic features and clinical behavior. The aim of this study was to evaluate the clinicopathologic significance of p63, Maspin and matrix metalloproteinase (MMP)-2 expression in MEC. Materials and Methods: In this retrospective study, immunohistochemical staining was performed to detect the expression of p63, Maspin and MMP-2 in 35 cases of MEC. In addition, to detect the presence of myoepithelial differentiation, smooth muscle actin (SMA) staining was performed. Data were analyzed using non-parametric tests. Results: P63 expression, intensity of staining for p63 and MMP-2 total score showed significant correlations with histologic grade (P=0.008, P=0.035 and P=0.003, respectively). The association between p63 expression and MMP-2 total score was significant (P=0.033). P63 positive cases did not express SMA. Conclusion: Findings suggest that p63 and MMP-2 may be useful prognostic indicators in MEC. MEC is devoid of myoepithelial cells.

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“…Tissue sections with 4 lm thickness were prepared and immunohistochemically stained with Ki-67 antibody (DAKO, Santa Clara, CA, USA) to assess cell proliferation ability. Immunohistochemical staining method was described previously (Guan et al 2015).…”

Section: Subcutaneous Xenograft Modelmentioning

confidence: 99%

“…It shows a strong capacity for local invasion and a high incidence of distant metastasis Dantas et al 2015). Our previous studies demonstrated that carcinoma-associated fibroblast (CAF) derived from SACC showed high levels of MMP2 and CXCL12 expression, which might be related to the aggressive growth behavior of SACC (Guan et al 2015). Furthermore, we demonstrated that SACCderived CAF led SACC invasion by creating an invasive track in the extracellular matrix (ECM) (Li et al 2016).…”

Section: Introductionmentioning

confidence: 97%

Establishment and characterization of a carcinoma-associated fibroblast cell line derived from a human salivary gland adenoid cystic carcinoma

Kong

Zhao

Shang

et al. 2018

Cell Communication & Adhesion

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Salivary gland adenoid cystic carcinoma (SACC) is one of the most common malignancies in the oral and maxillofacial region. Carcinoma-associated fibroblast (CAF) is an important component in the tumor microenvironment and participates in SACC progression. In this study, we established a CAF cell line derived from a human SACC and named it CAF-SA. It was identified that CAF-SA expressed typical CAF biomarkers. Then, we studied the cellular communications between CAF-SA, tumor cells and endothelial cells. It was found that CAF-SA promoted the migration, invasion, and proliferation of SACC tumor cells in vitro. In addition, tube formation by endothelial cells was enhanced by CAF-SA. In vivo experiment showed that SACC cells formed larger xenografts in nude mice when they were transplanted with CAF-SA. Overall, we demonstrated that CAF-SA exhibited the most important defining feature of CAF by promoting cancer progression.

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Myofibroblasts from salivary gland adenoid cystic carcinomas promote cancer invasion by expressing MMP2 and CXCL12

Abstract: This study demonstrated the presence of myofibroblasts in ACC. Myofibroblasts might be related to the aggressive growth behaviour of ACC, owing to their high levels of expression of MMP2 and CXCL12.

Cited by 17 publications

References 43 publications

Carcinoma-Associated Fibroblasts Lead the Invasion of Salivary Gland Adenoid Cystic Carcinoma Cells by Creating an Invasive Track

Carcinoma-Associated Fibroblasts Lead the Invasion of Salivary Gland Adenoid Cystic Carcinoma Cells by Creating an Invasive Track

Clinicopathologic Significance of P63, Maspin and MMP-2 Expression in Mucoepidermoid Carcinoma

Establishment and characterization of a carcinoma-associated fibroblast cell line derived from a human salivary gland adenoid cystic carcinoma

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