Carcinoma-Associated Fibroblasts Lead the Invasion of Salivary Gland Adenoid Cystic Carcinoma Cells by Creating an Invasive Track

Li, Jiao; Jia, Zhuqiang; Kong, Jing; Zhang, Fuyin; Fang, Shimeng; Li, Xiaojie; Li, Wuwei; Yang, Xuesong; Luo, Yong; Lin, Bingcheng; Liu, Tingjiao

doi:10.1371/journal.pone.0150247

Cited by 48 publications

(46 citation statements)

References 33 publications

(42 reference statements)

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“…This study provided a new model of CAF-mediated cancer cell invasion, in which CAFs physically remodel the matrix, allowing cancer cells to invade while still maintaining their epithelial properties [35]. A recent study made similar observations using salivary gland adenoid cystic carcinoma (ACC) cells [36]. Utilizing a microfluidic device, they co-cultured ACC cells and CAFs in a chamber that was separated from the chemoattractant (20% FBS) by Matrigel.…”

Section: Cafs Promote Cancer Cell Invasion By Modifying the Stromal Ecmmentioning

confidence: 94%

“…CAFs invaded the Matrigel using MMP activity and ACC cells followed behind them (Figure 2B). However, in this study, blocking the CXCL12/CXCR4 pathway inhibited cancer cell invasion into the tracks generated by CAFs, suggesting that signaling between CAFs and cancer cells drives ACC cell invasion [36]. …”

Section: Cafs Promote Cancer Cell Invasion By Modifying the Stromal Ecmmentioning

confidence: 99%

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Cancer-associated fibroblasts modulate growth factor signaling and extracellular matrix remodeling to regulate tumor metastasis

Erdogan

Webb

2017

Biochemical Society Transactions

398

317

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Cancer-associated fibroblasts (CAFs) are major components of the surrounding stroma of carcinomas that emerge in the tumor microenvironment as a result of signals derived from the cancer cells. Biochemical cross-talk between cancer cells and CAFs as well as mechanical remodeling of the stromal extracellular matrix (ECM) by CAFs are important contributors to tumor cell migration and invasion, which are critical for cancer progression from a primary tumor to metastatic disease. In this review, we discuss key paracrine signaling pathways between CAFs and cancer cells that promote cancer cell migration and invasion. In addition, we discuss physical changes that CAFs exert on the stromal ECM to facilitate migration and invasion of cancer cells.

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Section: Cafs Promote Cancer Cell Invasion By Modifying the Stromal Ecmmentioning

confidence: 94%

Section: Cafs Promote Cancer Cell Invasion By Modifying the Stromal Ecmmentioning

confidence: 99%

Cancer-associated fibroblasts modulate growth factor signaling and extracellular matrix remodeling to regulate tumor metastasis

Erdogan

Webb

2017

Biochemical Society Transactions

398

317

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“…Primary human normal fibroblasts (NF) were isolated as described previously and used as a control [28]. MCF7 and NF were cultured in Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (Hyclone, Logan, UT, USA).…”

Section: Methodsmentioning

confidence: 99%

Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification

et al. 2017

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Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more exosomes in serum than normal cells and these exosomes overexpress a certain number of cancer-related biomarkers. However, capture and biomarker analysis of exosomes for clinical application are technically challenging. In this study, we developed a microfluidic chip for immunocapture and quantification of circulating exosomes from small sample volume and applied this device in clinical study. Circulating EpCAM-positive exosomes were measured in 6 cases breast cancer patients and 3 healthy controls to assist diagnosis. A significant increase in the EpCAM-positive exosome level in these patients was detected, compared to healthy controls. Furthermore, we quantified circulating HER2-positive exosomes in 19 cases of breast cancer patients for molecular classification. We demonstrated that the exosomal HER2 expression levels were almost consistent with that in tumor tissues assessed by immunohistochemical staining. The microfluidic chip might provide a new platform to assist breast cancer diagnosis and molecular classification.

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“…CAFs also secrete various chemokines and cytokines that can affect tumor and other stromal cells, leading to cancer growth and invasion. In addition, these factors can stimulate angiogenesis around the tumor and draw bone marrow-derived cells or immune cells around the tumor (16,17). MMPs as extracellular matrix (ECM) degrading enzymes are also partly derived from CAFs (18).…”

Section: Discussionmentioning

confidence: 99%

Fascin is involved in cancer cell invasion and is regulated by stromal factors

et al. 2018

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The tumor microenvironment plays an important role in cancer growth, invasion and metastasis. The stroma surrounding a tumor is known to contain a variety of factors that can increase angiogenesis, cancer growth and tumor progression. The aim of the present study was to determine the role of fascin in cancer growth and invasion and identify stromal factors involved in cancer progression. A fascin-depleted cell line (fascin dep ) was used to observe the role of fascin in cancer invasion. Compared with wild-type Mock cells, cancer cell invasion in Matrigel-coated Transwell and three-dimensional (3D) culture system were reduced by fascin depletion. Tumor cell growth in vivo was also significantly reduced in mice injected with fascin dep cells. Notably, fascin expression was increased during Transwell invasion with Matrigel compared to Transwell invasion without Matrigel. TGF-β1, EGF and IL-1β significantly stimulated fascin expression. Such increased expression of fascin was also observed in cultured cells using conditioned media (CM) from cancer-associated fibroblasts (CAFs). However, no significant change in fascin expression was observed using CM from normal fibroblasts (NFs). Stimulated expression of fascin by Matrigel and CAFs was reduced by biological specific inhibitor of TGF-β1, EGF and IL-1β. Compared with wild-type Mock cells, the fascin dep cell line showed low RhoA and NF-κB activity, suggesting that RhoA and NF-κB signals are involved in fascin expression. In conclusion, stromal factors are involved in cancer invasion and progression by activating intracellular signaling of cancer cells to increase fascin expression. Fascin is involved in cancer cell invasion and is regulated by stromal factors

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Carcinoma-Associated Fibroblasts Lead the Invasion of Salivary Gland Adenoid Cystic Carcinoma Cells by Creating an Invasive Track

Cited by 48 publications

References 33 publications

Cancer-associated fibroblasts modulate growth factor signaling and extracellular matrix remodeling to regulate tumor metastasis

Cancer-associated fibroblasts modulate growth factor signaling and extracellular matrix remodeling to regulate tumor metastasis

Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification

Fascin is involved in cancer cell invasion and is regulated by stromal factors

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