Multivariate analysis of backcross progeny of Passiflora L. (Passifloraceae) for pre-breeding genotype selection

Abstract

ABSTRACT. The Ward-MLM procedure was used to evaluate genetic variation in four backcross progenies and in their parents, hybrid F1 HD13 and donor parent Passiflora sublanceolata. Sixteen quantitative descriptors and five qualitative characteristics of relevance to ornamental flower production were assessed. Using the pseudo-F and pseudo-T² criteria, we identified four groups among these plants in two evaluation periods. In both evaluations, the BC 1 plants showed greater dissimilarity to their recurrent parent, but showed high genetic similarity with the P. sublanceolata parent. The first two canonical variables produced by the Ward-MLM procedure accounted for over 90% of the variation in both evaluation periods, enabling the representation of diversity through two-dimensional graphics. Groups II and IV were formed in the first assessment period. Groups I and IV formed in the second period and showed plants with selection potential. We found that it was essential to use both qualitative and quantitative variables for this analysis. Assessments of quantitative descriptors indicate that the 15377 Genetic diversity for Passiflora breeding ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15376-15389 (2015) selection of BC 1 plants can be performed in any of the four progenies. Because of the similarities observed for some floral descriptors between BC 1 and the P. sublanceolata parent, a second generation backcross was not recommended. However, the selection of BC 1 plants for evaluation and direct use as an ornamental cultivar, or as a resource in other breeding programs, can be recommended.

Agronomic potential of BC1F2 populations of Santa Cruz dwarf tomato plants

“…Agronomy, v. 45, e56482, 2023 In general, one backcross was effective in increasing agronomic performance of the BC 1 F 2 dwarf populations (Figure 1). Other satisfactory results derived from the first backcross were observed in passion fruit (Melo, Souza, Sousa, Viana, & Santos, 2015), eggplant (García-Fortea et al, 2019), and tomato (Finzi et al, 2020). Thus, as in a previous study performed by Gonçalves Neto et al (2010), performing two more backcrosses is suggested, as this will result in lines with commercial standard fruit and subsequent attainment of hybrids of the Santa Cruz type from dwarf lines, as obtained by Finzi et al (2017a).…”

Cucumber mosaic virus resistance and reproductive biology of brazilian melon accessions

“…Beyond the analysis of variance (ANOVA) for quantitative floral descriptors, Ward-MLM multivariate analysis was also performed. The Ward-MLM multivariate procedure was performed using the distance matrix obtained by the logarithmic function of Gower coefficient (Gower, 1971), according to the method proposed by MELO et al (2015) on SAS Software analysis platform (SAS, 2000). The average values of the quantitative variables were included in the analysis together with the qualitative access data.…”

“…The Ward-MLM multivariate strategy allowed to verify variability and quantify genetic diversity based on floral, reproductive and CMV resistance descriptors, including quantitative and qualitative descriptors. The selection and establishment of descriptors for the study of phenotypic variation and its inference on genetic diversity by the Ward-MLM strategy have been widely used in various crops and plant groups (CAMPOS et al, 2013;MELO et al, 2015;GONÇALVES et al, 2018). However, this is the first study that uses floral, reproductive and biotic stress resistance data in the joint multivariate analysis.…”

“…The use in multivariate analysis in hybrid plants, reflecting the expected clustering according to the progenitors, has also been observed in other plant groups. For example, the species Passiflora sublanceolata (Killip) MacDougal and Passiflora foetida L., together with different hybrid progenies and backcrosses derived from them, all with potential for ornamental cultivar development, were analyzed for floral descriptors by the Ward-MLM technique, also presenting clusters consistent with their expected genetic diversity based on the crosses performed (MELO et al, 2015).…”

“…Ward-MLM multivariate analysis can be used to define strategies for plant breeding, such as contributing to the selection of promising progenitors (MELO et al, 2015). The distribution of potentially resistant plants in groups 4 and 6 suggests that these two groups may present promising plants for improving plant resistance to CMV.…”