Morphoagronomic characterization and genetic diversity of a Brazilian okra [Abelmoschus esculentus (L.) Moench] panel

Silva, Edgard Henrique Costa; Franco, Carolina Andrade; Candido, Willame dos Santos; Braz, Leila Trevisan

doi:10.1007/s10722-020-00992-7

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…However, low production limits the development of the okra industries. For a long time, few okra cultivars have been bred, which has contributed to yield stagnation [ 5 ]. Developing modern cultivars with significant heterosis based on cytoplasmic male sterility associated with various chimeric open reading frames in the plant mitochondrial genome (mtDNA) is common among crops.…”

Section: Introductionmentioning

confidence: 99%

The complete mitochondrial genome of okra (Abelmoschus esculentus): using nanopore long reads to investigate gene transfer from chloroplast genomes and rearrangements of mitochondrial DNA molecules

et al. 2022

BMC Genomics

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Background Okra (Abelmoschus esculentus L. Moench) is an economically important crop and is known for its slimy juice, which has significant scientific research value. The A. esculentus chloroplast genome has been reported; however, the sequence of its mitochondrial genome is still lacking. Results We sequenced the plastid and mitochondrial genomes of okra based on Illumina short reads and Nanopore long reads and conducted a comparative study between the two organelle genomes. The plastid genome of okra is highly structurally conserved, but the mitochondrial genome of okra has been confirmed to have abundant subgenomic configurations. The assembly results showed that okra’s mitochondrial genome existed mainly in the form of two independent molecules, which could be divided into four independent molecules through two pairs of long repeats. In addition, we found that four pairs of short repeats could mediate the integration of the two independent molecules into one complete molecule at a low frequency. Subsequently, we also found extensive sequence transfer between the two organelles of okra, where three plastid-derived genes (psaA, rps7 and psbJ) remained intact in the mitochondrial genome. Furthermore, psbJ, psbF, psbE and psbL were integrated into the mitochondrial genome as a conserved gene cluster and underwent pseudogenization as nonfunctional genes. Only psbJ retained a relatively complete sequence, but its expression was not detected in the transcriptome data, and we speculate that it is still nonfunctional. Finally, we characterized the RNA editing events of protein-coding genes located in the organelle genomes of okra. Conclusions In the current study, our results not only provide high-quality organelle genomes for okra but also advance our understanding of the gene dialogue between organelle genomes and provide information to breed okra cultivars efficiently.

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Section: Introductionmentioning

confidence: 99%

The complete mitochondrial genome of okra (Abelmoschus esculentus): using nanopore long reads to investigate gene transfer from chloroplast genomes and rearrangements of mitochondrial DNA molecules

et al. 2022

BMC Genomics

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“…Knowledge on genetic diversity plays an important role in breeding programs to improve agronomic traits and resistance to environmental stresses (ABD EL-FATTAH; HARIDY; ABBAS, 2020). Some cultivars have been released recently, including hybrids, but one open-pollinated cultivar ('Santa Cruz 47') has been dominating the market over 45 years (SILVA et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Genetic and Morphological Descriptors to Access Brazilian Okra Genotypes Diversity

et al. 2022

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Information of the variation for important morphological and physiological traits of okra is still limited. Molecular analysis is an important additional tool in germplasm characterization studies. The study aimed to evaluate the performance of the growth and yield of 20 pre-commercial okra accessions to identify molecular markers’ association with morphological traits. Nineteen morphological traits were measured with five qualitative and 14 quantitative descriptors. For analysis of genetic patterns Random Amplified Polymorphic DNA (RAPD) markers were used with nine primers and 24 usable bands. The genetic dissimilarity was evaluated based in morphological and genetic matrices. Also, graphical representation of genetic distances was obtained by UPGMA and Tocher’s optimization method. The morphological characterization of the accessions detected polymorphism for all evaluated traits. RAPD markers were efficient in detecting genetic variability among okra accessions. For the primers used in the experiment, only OPE10 did not amplify the DNA strand. The other eight primers produced a total of 35 bands, in which 25 were polymorphic and ten were monomorphic. The morphological traits and molecular markers identified wide genetic variability among the 20 okra accessions, indicating successful crosses in breeding programs and isolating some interesting materials. Morphological and molecular cluster analyses were complementary and helped in the genotype selection. Molecular analysis indicated some divergent accessions that were not found in morphological analysis, which could highlight some materials that have a desirable trait, that is difficult and highly costly to access in field experiments.

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“…Nine genotypes were separated into two groups based on genetic divergence (Silva et al 2021). The seven genotypes of the first group (CNPH-19, CNPH-24, CNPH-11, CNPH-09, CNPH-05, CNPH-02, and CNPH-35, namely genitors 1 to 7) were crossed with the two genotypes of the second group (Santa Cruz 47 and CNPH-42, namely genitor 8 and 9, respectively) and vice versa, resulting in 14 hybrids and 14 reciprocal hybrids.…”

Section: Parents and Partial Diallel Crossesmentioning

confidence: 99%

Obtaining okra hybrids through partial diallel analysis

Silva

Candido

Braz

2021

Crop Breed. Appl. Biotechnol.

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The exploration of heterosis can be an efficient alternative to increase okra yield. The partial diallel strategy optimizes resources by guiding crosses based on genetic diversity between various genotypes. This study aimed to identify potential hybrid combinations through diallel analysis in comparison with commercial cultivars. The hybrids were evaluated by means of a partial diallel scheme based on the genetic divergence of the genotypes. Fourteen hybrids and their reciprocals were evaluated in the winter and summer conditions of Jaboticabal -SP for yield, number of fruits and precocity. No line showed significant general combining ability. The reciprocal effect was not significant for the evaluated characteristics. Four hybrid combinations showed significant and positive specific combining ability for number of fruits, which were considered promising hybrids. In the winter, the experimental hybrids 1 x 8 and 3 x 8 showed superior performance compared to the commercial hybrids. No significant differences were observed in the summer.

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Morphoagronomic characterization and genetic diversity of a Brazilian okra [Abelmoschus esculentus (L.) Moench] panel

Cited by 8 publications

References 26 publications

The complete mitochondrial genome of okra (Abelmoschus esculentus): using nanopore long reads to investigate gene transfer from chloroplast genomes and rearrangements of mitochondrial DNA molecules

The complete mitochondrial genome of okra (Abelmoschus esculentus): using nanopore long reads to investigate gene transfer from chloroplast genomes and rearrangements of mitochondrial DNA molecules

Genetic and Morphological Descriptors to Access Brazilian Okra Genotypes Diversity

Obtaining okra hybrids through partial diallel analysis

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