Multiplexed single-cell morphometry for hematopathology diagnostics

Tsai, Albert; Glass, David R.; Juntilla, Marisa M.; Hartmann, Felix J.; Oak, Jean; Fernandez‐Pol, Sebastian; Ohgami, Robert S.; Bendall, Sean C.

doi:10.1038/s41591-020-0783-x

Cited by 34 publications

(35 citation statements)

References 34 publications

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“…For example, use of microchannel-based fluidic systems and adoption of adjustable scattering angles (Rossi et al, 2019), inclusion of generic dyes (e.g. MitoTracker) measuring mitochondrial abundance/activity (Baron et al, 2019), or biomarkers possibly surrogating biophysical properties (Tsai et al, 2020; Walsh et al, 2019) to improve lymphocyte subsets identification.…”

Section: Discussionmentioning

confidence: 99%

Label-free lymphocytes reconstitution using side scatter for optimal T cell manufacturing

Luo

et al. 2020

Preprint

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SUMMARYLymphocyte biology research commonly involves purification of lymphocyte subpopulations by fluorescence-activated cell sorting (FACS) or immunomagnetic separation (IMS), both of which typically rely on antibody labeling of validated cell markers. Methods enabling label-free segregation of lymphocyte subpopulations would be invaluable with regard to less-perturbation, simplicity and cost-effectiveness. Here, we introduce TRuST, a label-free approach for T cell reconstitution using side-scatter (SSC). TRuST-sorted SSClow cells enrich for CD4+ T and naïve T cells, while SSChigh cells enrich for CD8+ T, NK and differentiated T cells. Enrichment purity can be improved by computational gate design. SSClow cells have superior expansion capacity and generate more central memory precursors with naïve-resembling cytokine responses. Moreover, we find that both T cell differentiation status and CD4/CD8 T ratio in the starting cellular material are critical attributes predicting T cell product quality and quantity. TRuST presents an effective and reliable technique for label-free lymphocytes selection and reconstitution.

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Section: Discussionmentioning

confidence: 99%

Label-free lymphocytes reconstitution using side scatter for optimal T cell manufacturing

Luo

et al. 2020

Preprint

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“…It is important to highlight the impact that this technique is likely to have in areas such as clinical medicine, where it already has been proposed as a powerful tool for the diagnosis of autoimmune and complex hematologic diseases [ 59 , 60 ]. Similarly, a recent expansion of CyTOF, known as Imaging Mass Cytometry (IMC), has been proposed in the field of personalised medicine [ 61 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

Characterizing Highly Cited Papers in Mass Cytometry through H-Classics

Zeo-Sánchez

Sánchez-Núñez

Stephens

et al. 2021

Biology

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Mass cytometry (CyTOF) is a relatively novel technique for the multiparametric analysis of single-cell features with an increasing central role in cell biology, immunology, pharmacology, and biomedicine. This technique mixes the fundamentals of flow cytometry with mass spectrometry and is mainly used for in-depth studies of the immune system and diseases with a significant immune load, such as cancer, autoimmune diseases, and viral diseases like HIV or the recently emerged COVID-19, produced by the SARS-CoV-2 coronavirus. The objective of this study was to provide a useful insight into the evolution of the mass cytometry research field, revealing the knowledge structure (conceptual and social) and authors, countries, sources, documents, and organizations that have made the most significant contribution to its development. We retrieved 937 articles from the Web of Science (2010–2019), analysed 71 Highly Cited Papers (HCP) through the H-Classics methodology and computed the data by using Bibliometrix R package. HCP sources corresponded to high-impact journals, such as Nature Biotechnology and Cell, and its production was concentrated in the US, and specifically Stanford University, affiliation of the most relevant authors in the field. HCPs analysis confirmed great interest in the study of the immune system and complex data processing in the mass cytometry research field.

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“…Applying a new form of mass cytometry, the so-called “multiplex single-cell morphometry” based on the identification of a dozen of intracellular molecules related to structure and function (including lamin A/C, beta-actin, lysozyme, the vesicle docking molecule VAMP-7), Bendall and coworkers [ 106 ] demonstrated distinct morphometric markers for each major hematologic cell type. Interestingly, lamin A/C helped to distinguish normal from mature neoplastic T-cells (T-PLL).…”

Section: Reviewmentioning

confidence: 99%

“…We recently demonstrated distinct 3D structural lamin A/C expression patterns in H and RS-cells [ 107 ] earlier identified with 2D immunohistochemistry [ 108 ]. Knowing that the nuclear 3D structure is related to the direct interaction of TRF2 with lamin A/C [ 109 ] and that loss of lamin A function increases chromatin dynamics in the nuclear interior [ 110 ] ( Figure 3 ), it might be worth to investigate the lymphocyte population of Hodgkin lymphoma lymph nodes by “multiplex single-cell morphometry” [ 106 ]. Though most of the lymphocytes surrounding H- and RS-cells are T-lymphocytes, B-lymphocytes are also present and few lymphocytes express lamin A/C in immunohistology.…”

Section: Reviewmentioning

confidence: 99%

“…As the 3D cancer cell nucleus is a dynamic structure whose end-stage alterations are mostly identifiable [ 117 ] it is mandatory to detect and to analyze the very early changes on the road to malignancy with recently developed super-resolution microscopic techniques [ 118 ], humanized mouse model systems [ 69 ], and mass cytometry of nuclear structural proteins [ 106 ].…”

Section: Reviewmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Pathogenesis of Hodgkin Lymphoma: Past, Present, Future

Bienz

Ramdani

Knecht

2020

IJMS

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Our understanding of the tumorigenesis of classical Hodgkin lymphoma (cHL) and the formation of Reed–Sternberg cells (RS-cells) has evolved drastically in the last decades. More recently, a better characterization of the signaling pathways and the cellular interactions at play have paved the way for new targeted therapy in the hopes of improving outcomes. However, important gaps in knowledge remain that may hold the key for significant changes of paradigm in this lymphoma. Here, we discuss the past, present, and future of cHL, and review in detail the more recent discoveries pertaining to genetic instability, anti-apoptotic signaling pathways, the tumoral microenvironment, and host-immune system evasion in cHL.

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Multiplexed single-cell morphometry for hematopathology diagnostics

Cited by 34 publications

References 34 publications

Label-free lymphocytes reconstitution using side scatter for optimal T cell manufacturing

Label-free lymphocytes reconstitution using side scatter for optimal T cell manufacturing

Characterizing Highly Cited Papers in Mass Cytometry through H-Classics

Molecular Pathogenesis of Hodgkin Lymphoma: Past, Present, Future

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