Multiple mechanisms of SDF-1 promoting VEGF-induced endothelial differentiation of mesenchymal stem cells

Wang, Mingke; Zou, Zhongmin

doi:10.1016/j.ijcard.2014.09.198

Cited by 11 publications

(5 citation statements)

References 14 publications

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“…Therefore, multiple methods of inducing MSCs to differentiate towards VECs have been used in our previous work, with the purpose of clarifying the relationship between DMSCs and angiogenesis in psoriatic lesion. Based on available knowledge, VEGF [7,17], SDF-1 [18], BMP-4 [14] and other factors were added into the differentiation medium, but the differentiation from DMSCs and BMSCs was not ideal in our research. In addition, Endothelial Cell Growth Medium-2 (EGM-2) (Lonza, USA, Lot.No.…”

Section: Discussionmentioning

confidence: 99%

Efficient differentiation of vascular endothelial cells from dermal-derived mesenchymal stem cells induced by endothelial cell lines conditioned medium

Zhou¹,

Niu²,

Liang³

et al. 2018

Preprint

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ObjectiveTo directionally-differentiate dermis-derived mesenchymal stem cells (DMSCs) into vascular endothelial cells (VECs) in vitro, providing an experimental basis for studies on the pathogenesis and treatment of vascular diseases.MethodsAfter separation by adherent culture, VEC line supernatant, vascular endothelial growth factor (VEGF), bone morphogenetic protein-4 and hypoxia were used for the differentiation of VECs from DMSCs. The cell type was authenticated by flow cytometry, matrigel angiogenesis assay in vitro, and immunofluorescent staining during differentiation. The VEGF concentration was investigated by enzyme-linked immunosorbent assay.ResultsAfter 28 days of differentiation, the cell surface marker CD31 was significantly positive (80%-90%) by flow cytometry in the VEC line-conditioned culture, which was significantly higher than in the other groups. Differentiated DMSCs had the ability to ingest Dil-ac-LDL and vascularize in the conditioned culture, but not in the other groups. In the VEC line supernatant, the concentration of VEGF was very low. The VEGF concentration changed along with the differentiation into VECs in the medium of the conditioned culture group.ConclusionVEC line supernatant can induce the differentiation of DMSCs into VECs, possibly through the pathway except VEGF.

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Section: Discussionmentioning

confidence: 99%

Efficient differentiation of vascular endothelial cells from dermal-derived mesenchymal stem cells induced by endothelial cell lines conditioned medium

Zhou¹,

Niu²,

Liang³

et al. 2018

Preprint

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show abstract

“…More interestingly, targeting both SDF-1 and VEGF-C by neutralizing antibodies resulted in an additive inhibitory effect on both tumor lymphangiogenesis and lymphatic metastasis [ 12 ]. Furthermore, it is hypothesized that SDF-1 and VEGF may have synergetic effect on endothelial differentiation through a positive feedback loop with mutual promotion [ 30 ]. Thus, to clarify the coeffect of SDF-1 and VEGF-C, we added both in the culture system, and the data revealed that costimulation with SDF-1 and VEGF-C displayed an additive effect on the LECs differentiation; however, no additive effect was observed on the LECs migration, which needs a further mechanism clarification.…”

Section: Discussionmentioning

confidence: 99%

Jin Fu Kang Oral Liquid Inhibits Lymphatic Endothelial Cells Formation and Migration

Wang

Tang

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Lung cancer is the leading cause of cancer-related deaths worldwide. Jin Fu Kang (JFK), an oral liquid prescription of Chinese herbal drugs, has been clinically available for the treatment of non-small cell lung cancer (NSCLC). Lymphangiogenesis is a primary event in the process of cancer development and metastasis, and the formation and migration of lymphatic endothelial cells (LECs) play a key role in the lymphangiogenesis. To assess the activity of stromal cell-derived factor-1 (SDF-1) and the coeffect of SDF-1 and vascular endothelial growth factor-C (VEGF-C) on the formation and migration of LECs and clarify the inhibitory effects of JFK on the LECs, the LECs were differentiated from CD34+/VEGFR-3+ endothelial progenitor cells (EPCs), and JFK-containing serums were prepared from rats. SDF-1 and VEGF-C both induced the differentiation of CD34+/VEGFR-3+ EPCs towards LECs and enhanced the LECs migration. Couse of SDF-1 and VEGF-C displayed an additive effect on the LECs formation but not on their migration. JFK inhibited the formation and migration of LECs, and the inhibitory effects were most probably via regulation of the SDF-1/CXCR4 and VEGF-C/VEGFR-3 axes. The current finding suggested that JFK might inhibit NSCLC through antilymphangiogenesis and also provided a potential to discover antilymphangiogenesis agents from natural resources.

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“…Xu et al (2007) reported that SDF-1α and VEGF from hypoxia-induced astrocytes could promote neural progenitor cells migration in vitro. Moreover, SDF-1α cooperated with VEGFmodulated circulating MSCs' recruitment and entrapment as a vital step of neovascularization, and SDF-1α increased VEGF expression to induce MSCs' differentiation into ECs from multiple approaches (Zhou et al, 2007;Wang and Zou, 2014). Among families of neurotrophic factors, BDNF and GDNF are found to be the major factors that improve stroke recovery and brain plasticity after ischemia (Béjot et al, 2011;Kuric et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Simvastatin combined with bone marrow stromal cells treatment activatesastrocytes to ameliorate neurological function after ischemic stroke in rats

Zhao¹,

Zhang²,

Chen³

et al. 2016

Turk J Biol

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Multiple mechanisms of SDF-1 promoting VEGF-induced endothelial differentiation of mesenchymal stem cells

Cited by 11 publications

References 14 publications

Efficient differentiation of vascular endothelial cells from dermal-derived mesenchymal stem cells induced by endothelial cell lines conditioned medium

Efficient differentiation of vascular endothelial cells from dermal-derived mesenchymal stem cells induced by endothelial cell lines conditioned medium

Jin Fu Kang Oral Liquid Inhibits Lymphatic Endothelial Cells Formation and Migration

Simvastatin combined with bone marrow stromal cells treatment activatesastrocytes to ameliorate neurological function after ischemic stroke in rats

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